Richard P. Haugland

TL;DR: The enzymatic determination of hydrogen peroxide can be accomplished with high sensitivity and specificity using N-acetyl-3, 7-dihydroxyphenoxazine (Amplex Red), a highly sensitive and chemically stable fluorogenic probe for the enzymatics determination of H2O2.

TL;DR: This work evaluated Alexa dyes compared with conventional dyes in applications using various conjugates, including those of goat anti-mouse IgG (GAM), streptavidin, wheat germ agglutinin (WGA), and concanavalin A (ConA).

TL;DR: The PicoGreen assay allowed the detection of 25 pg/ml ds DNA, surpassing the sensitivity achieved with Hoechst 33258 by 400-fold, and showed greater dsDNA:RNA selectivity than HoeChSt 33258 in low ionic strength buffer and better dSDNA:single-stranded DNA selectivity in 1 M NaCl.

TL;DR: The synthesis, proof of structure, and the absorption and fluorescence properties of two new unsymmetrical cyanine dyes, thiazole orange dimer and oxazole yellow dimer are reported, which form highly fluorescent complexes with double-stranded DNA (dsDNA) with greater than 1000-fold fluorescence enhancement.

TL;DR: TMA-DPH, a cationic analogue of diphenylhexatriene (DPH), has photophysical properties that are generally similar to those of DPH, and should prove useful in the study of the dynamics of phospholipid monolayers, e.g., in native or reconstituted lipoproteins.