V
Vijay K. Chaudhary
Researcher at University of Delhi
Publications - 151
Citations - 6068
Vijay K. Chaudhary is an academic researcher from University of Delhi. The author has contributed to research in topics: Pseudomonas exotoxin & Fusion protein. The author has an hindex of 38, co-authored 123 publications receiving 5900 citations. Previous affiliations of Vijay K. Chaudhary include United States Army Medical Research Institute of Infectious Diseases & Laboratory of Molecular Biology.
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Journal ArticleDOI
A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin
Vijay K. Chaudhary,Cary L. Queen,Richard P. Junghans,Thomas A. Waldmann,Desmond J. Fitzgerald,Ira Pastan +5 more
TL;DR: Anti-Tac(Fv)–PE40 was very cytotoxic to two interleukin-2 receptor-bearing human cell lines but was not cytotoxicity to receptor-negative cells, suggesting that the construction and expression of this single chain antibody toxin fusion protein in E coli is likely to be influenced by its carrier status.
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Recombinant toxins as novel therapeutic agents.
TL;DR: Information is provided on how to identify the phytochemical properties of the building blocks of lmmunotoxins and how to remove them from the environment using a non-volatile substance such as diamorphine.
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Selective killing of HIV-infected cells by recombinant human CD4- Pseudomonas exotoxin hybrid protein
Vijay K. Chaudhary,Tamio Mizukami,Thomas R. Fuerst,David J. FitzGerald,Bernard Moss,Ira Pastan,Edward A. Berger +6 more
TL;DR: The purification and characterization of a recombinant protein produced in Escherichia coli that contains the HIV-binding portion of the human CD4 molecule linked to active regions of Pseudomonas exotoxin A is described, which represents a promising novel therapeutic agent for the treatment of AIDS.
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Pseudomonas exotoxin contains a specific sequence at the carboxyl terminus that is required for cytotoxicity
TL;DR: Mutational analysis indicates that a basic amino acid at 609, acidic amino acids at 610 and 611, and a leucine at 612 are required for full cytotoxic activity, suggesting that a common factor may be involved in intoxication of cells by PE and retention of proteins in the lumen of the endoplasmic reticulum.
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Processing of Pseudomonas exotoxin by a cellular protease results in the generation of a 37,000-Da toxin fragment that is translocated to the cytosol.
TL;DR: The 37-kDa fragment appears to be essential for toxicity since mutant PE molecules that do not produce this fragment, or cannot deliver it to the cytosol, fail to kill cells.