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Wayne A. Halsey
Researcher at University of Colorado Boulder
Publications - 7
Citations - 344
Wayne A. Halsey is an academic researcher from University of Colorado Boulder. The author has contributed to research in topics: Telomere-binding protein & DNA. The author has an hindex of 6, co-authored 7 publications receiving 324 citations. Previous affiliations of Wayne A. Halsey include National Institute of Standards and Technology.
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Journal ArticleDOI
Stabilization of an optical microscope to 0.1 nm in three dimensions
Ashley R. Carter,Gavin M. King,Theresa A. Ulrich,Wayne A. Halsey,David Alchenberger,Thomas T. Perkins +5 more
TL;DR: This work overcame mechanical drift and achieved atomic-scale stabilization (0.1 nm) of an optical microscope in 3D by measuring the position of a fiducial mark coupled to the microscope cover slip using back-focal-plane (BFP) detection and correcting for the drift using a piezoelectric stage.
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Delineation of the high‐affinity single‐stranded telomeric DNA‐binding domain of Saccharomyces cerevisiae Cdc13
TL;DR: A previously identified domain of Cdc13 encompassing amino acids 451-694 retains the single-stranded DNA-binding properties of the full-length protein; however, this domain contains a large unfolded region identified in heteronuclear NMR experiments.
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Site-directed mutagenesis reveals the thermodynamic requirements for single-stranded DNA recognition by the telomere-binding protein Cdc13.
TL;DR: The essential Saccharomyces cerevisiae protein Cdc13 binds the conserved single-stranded overhang at the end of telomeres and mediates access of protein complexes involved in both end-capping and telomerase activity, which exhibits both high affinity and sequence specificity for the GT-rich sequences present at yeast telomere.
Journal ArticleDOI
TFIIA changes the conformation of the DNA in TBP/TATA complexes and increases their kinetic stability.
Aaron R. Hieb,Wayne A. Halsey,Meredith D. Betterton,Thomas T. Perkins,Jennifer F. Kugel,James A. Goodrich +5 more
TL;DR: TFIIA induces a conformational change within the TBP/TATA complex that enhances its stability under both in vitro and physiological salt conditions, and a refined model for the effect that TFIIA has on DNA conformation is presented that takes into account potential changes in bend angle as well as twist angle.
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Identification of the determinants for the specific recognition of single-strand telomeric DNA by Cdc13.
TL;DR: Understanding of telomere recognition by the Cdc13-DBD is expanded and of the unique molecular recognition properties of ssDNA binding is expanded.