Wei Gui

TL;DR: Combined flow cytometry, growth rate measurements, and NMR- and mass spectrometry-based metabolomics are combined to systematically profile the impact of bile acids on the microbiome using in vitro and in vivo models providing validation of previous observation and new insights into the interaction of biles acids with the microbiome and mechanisms related to bile acid tolerance.

TL;DR: In vitro and in vivo data suggest that BBR directly affects bacteria to alter bile acid metabolism and activate FXR signaling, providing new insights into the link between intestinal bacteria, nuclear receptor signaling, and xenobiotics.

TL;DR: The detection technologies currently used for bile acid identification and quantification are reviewed and the advantages and disadvantages of these analytical techniques with respect to sensitivity, specificity, robustness, and ease of use are discussed.

TL;DR: WB feeding resulted in shifts in the microbiota that induced IGN and improved glucose homeostasis, and WB-fed lean mice had a small but significant improvement in glucose sensitivity.

TL;DR: This study systematically examined the direct effects of POPs on the microbiota using metatranscriptomics and NMR- and mass spectrometry-based metabolomics combined with flow cytometry and growth rate measurements to establish possible microbial toxicity endpoints.