THE DESIGN AND ANALYSIS OF EXPERIMENTS. By Oscar Kempthorne. New York, John Wiley and Sons, Inc., 1952. 631 pp. $8.50. This book by a teacher of statistics (as well as a consultant for \"experimenters\") is a comprehensive study of the philosophical background for the statistical design of experiment. It is necessary to have some facility with algebraic notation and manipulation to be able to use the volume intelligently. The problems are presented from the theoretical point of view, without such practical examples as would be helpful for those not acquainted with mathematics. The mathematical justification for the techniques is given. As a somewhat advanced treatment of the design and analysis of experiments, this volume will be interesting and helpful for many who approach statistics theoretically as well as practically. With emphasis on the \"why,\" and with description given broadly, the author relates the subject matter to the general theory of statistics and to the general problem of experimental inference. MARGARET J. ROBERTSON

The Design and Analysis of Experiments

/pdf/self-assembled-monolayers-of-thiolates-on-metals-as-a-form-1neb0hj3k4.pdf

Self-assembled monolayers of thiolates on metals as a form of nanotechnology.

We report here that cells co-purifying with mesenchymal stem cells--termed here multipotent adult progenitor cells or MAPCs--differentiate, at the single cell level, not only into mesenchymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro. When injected into an early blastocyst, single MAPCs contribute to most, if not all, somatic cell types. On transplantation into a non-irradiated host, MAPCs engraft and differentiate to the haematopoietic lineage, in addition to the epithelium of liver, lung and gut. Engraftment in the haematopoietic system as well as the gastrointestinal tract is increased when MAPCs are transplanted in a minimally irradiated host. As MAPCs proliferate extensively without obvious senescence or loss of differentiation potential, they may be an ideal cell source for therapy of inherited or degenerative diseases.

/pdf/pluripotency-of-mesenchymal-stem-cells-derived-from-adult-200s4qlizf.pdf

Pluripotency of mesenchymal stem cells derived from adult marrow

Overview of membrane science and technology membrane transport theory membrane and modules concentration polarization reverse osmosis ultrafiltration microfiltration gas separation pervaporation ion exchange membrane processes - electrodialysis carrier facilitated transport medical applications of membranes other membranes processed.

Membrane Technology and Applications

N G van Kampen 1981 Amsterdam: North-Holland xiv + 419 pp price Dfl 180 This is a book which, at a lower price, could be expected to become an essential part of the library of every physical scientist concerned with problems involving fluctuations and stochastic processes, as well as those who just enjoy a beautifully written book. It provides an extensive graduate-level introduction which is clear, cautious, interesting and readable.

https://iopscience.iop.org/article/10.1088/0031-9112/34/4/040/pdf

Stochastic Processes in Physics and Chemistry

We have derived from normal human, mouse, and rat postnatal bone marrow primitive, multipotent adult progenitor cells (MAPCs) that can differentiate into most mesodermal cells and neuroectodermal cells in vitro and into all embryonic lineages in vivo. Here, we show that MAPCs can also differentiate into hepatocyte-like cells in vitro. Human, mouse, and rat MAPCs, cultured on Matrigel with FGF-4 and HGF, differentiated into epithelioid cells that expressed hepatocyte nuclear factor-3β (HNF-3β), GATA4, cytokeratin 19 (CK19), transthyretin, and α-fetoprotein by day 7, and expressed CK18, HNF-4, and HNF-1α on days 14–28. Virtually all human, as well as a majority of rodent cells stained positive for albumin and CK18 on day 21; 5% (rodent) to 25% (human) cells were binucleated by day 21. These cells also acquired functional characteristics of hepatocytes: they secreted urea and albumin, had phenobarbital-inducible cytochrome p450, could take up LDL, and stored glycogen. MAPCs, which can be expanded in vitro and maintained in an undifferentiated state for more than 100 population doublings, can thus differentiate into cells with morphological, phenotypic, and functional characteristics of hepatocytes. MAPCs may therefore be an ideal cell for in vivo therapies for liver disorders or for use in bioartificial liver devices.

/pdf/multipotent-adult-progenitor-cells-from-bone-marrow-16gm7ddvor.pdf

Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells.

Recombinant protein therapeutics have changed the face of modern medicine in the past decade, and they continue to provide innovative and effective therapies for numerous previously refractory illnesses. Today, they are used in the treatment of a variety of human diseases, ranging from cancers to infertility. These proteins are generally synthesized by large-scale cultivation of genetically engineered “host” cells, which harbor artificially transfected genes encoding for the proteins of interest. For protein therapeutics to be effective, they must be synthesized in biologically active forms, requiring proper folding and post-translational modifications. In many cases, this includes glycosylation, a type of modification where certain carbohydrate moieties are added to specific amino acid residues of the protein. Glycoproteins, as these are usually called, are generally synthesized in mammalian cells, because common microbial hosts like Escherichia coli lack the requisite machinery to synthesize appropriate glycoforms. Several rodentor human-derived cells like 3T3, CHO, BHK, HeLa and HepG2 are frequently used in biomedical research for heterologous protein expression. Despite the availability of a plenitude of cell lines, nearly 70% of all recombinant protein therapeutics produced today are made in Chinese Hamster Ovary (CHO) cells. The current annual sales for biologics produced using CHO cells alone exceed US$30 billion worldwide. The first recombinant therapeutic protein produced in mammalian cells, tissue plasminogen activator (r-tPA, Activase) synthesized using CHO cells, was approved for clinical use in 1987. This marked the beginning of an array of highly successful CHO-based therapeutics (Table), which continue to revolutionize the field of medicine to this day. The knowledge and expertise amassed over the past two decades will most certainly ensure that CHO cells continue to remain the industry's premier workhorse for therapeutic protein production, at least in the near future. In this article, we revisit the evolution of CHO cells, tracing from their origins to the development of current production cell lines, and discuss some important aspects that make them robust and versatile protein expression hosts. Unlike some of its rodent relatives (mouse and rat), the generation of genomic resources for Chinese hamster has been quite limited. Genomic and proteomic tools using these resources can potentially aid in understanding and improving recombinant protein production processes leading to a significant enhancement in the speed with which these therapeutics transition from laboratory molecules to life-saving medicines. We, therefore, devote special attention to the value and need for such information in meeting the challenges of future research and manufacturing processes.

Recombinant protein therapeutics from CHO cells : 20 years and counting

This book offers an introduction to the separation and purification of biochemicals. Bridges two scientific cultures, providing an introduction to bioseparations. The authors supplement the ideas by worked examples. Discusses removal of insolubles, product isolation, purification and polishing.

Bioseparations: Downstream Processing for Biotechnology

Culture Systems for Pluripotent Stem Cells

Preface Cell Culture Technology: An Overview Recombinant DNA Technology and Cell Line Development Medium Development Cell Metabolism Protein Glycosylation Cell Culture Bioreactors Aeration, Mixing and Hydrodynamics in Bioreactors Instrumentation and Process Control Cell Culture Kinetics and Modeling Fed-Batch Cultivation Of Mammalian Cells For Production Of Recombinant Proteins Optimization of High Density Perfusion Bioreactors Cell Separation And Product Capture Downstream Processing Formulation, Finishing, and Packaging Validation Of Cell Culture Based Processes & Qualification Of Associated Equipment And Facility Facility Design Transient Expression of Proteins Large-Scale Propagation of Insect Cells Advances In Adult Stem Cell Culture Ex Vivo Culture Of Hematopoietic And Mesenchymal Stem Cells For Tissue Engineering And Cell-Based Therapies

https://www.progenericos.org.br/upload/Sadettin_Ozturk_Aug2009_UFRJ.pdf

Wei Shou Hu

Papers

Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells.

Recombinant protein therapeutics from CHO cells : 20 years and counting

Bioseparations: Downstream Processing for Biotechnology

Culture Systems for Pluripotent Stem Cells

Cell culture technology for pharmaceutical and cell-based therapies