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Wentao Shen
Researcher at Chinese Academy of Tropical Agricultural Sciences
Publications - 35
Citations - 491
Wentao Shen is an academic researcher from Chinese Academy of Tropical Agricultural Sciences. The author has contributed to research in topics: Papaya ringspot virus & Gene. The author has an hindex of 12, co-authored 28 publications receiving 355 citations.
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High-throughput construction of intron-containing hairpin RNA vectors for RNAi in plants.
TL;DR: A novel restriction-ligation approach that provides a simple but efficient construction of intron-containing hpRNA (ihpRNA) vectors and demonstrates the utility of the ihpRNA constructs generated with pRNAi-GG vector for the effective silencing of various individual endogenous and exogenous marker genes as well as two genes simultaneously.
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Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning.
TL;DR: The cloning technique known as “Nimble Cloning” uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the vector and generate 3′-overhangs simultaneously.
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Helper component-proteinase (HC-Pro) protein of Papaya ringspot virus interacts with papaya calreticulin
TL;DR: It is proposed that the upregulated expression of PaCRT mRNA may be an early defence-related response toPRSV infection in the host plant, and that interaction between PRSV HC-Pro and PaCRt may be involved in plant calcium signalling pathways which could interfere with virus infection or host defence.
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Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning.
TL;DR: To the authors' knowledge, this is the first report of the construction of an infectious full-length cDNA clone of PLDMV and the splicing of intron-containing transcripts following mechanical inoculation.
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Cloning and expression analysis of phytoene desaturase and ζ-carotene desaturase genes in Carica papaya
TL;DR: Results indicated that the differential accumulation of carotenoids in red- and yellow-fleshed papaya varieties might be partly explained by the transcriptional level of CpPDS and CpZDS.