Werner Aufsatz

TL;DR: It is shown here that transcriptional gene silencing accompanied by de novo methylation of a target promoter in plants can be triggered by a double‐stranded RNA containing promoter sequences.

TL;DR: It is demonstrated that DRM activity is required for the initial establishment of RdDM in all sequence contexts including CpG, CpNpG and asymmetric sites, and that DRM and CMT3 act redundantly to maintain non-CpG methylation.

TL;DR: It is reported here that DRD2 and DRD3 correspond to the second-largest subunit and largest subunit, respectively, of a fourth class of DNA-dependent RNA polymerase (polymerase IV) that is unique to plants.

TL;DR: The results are consistent with a model in which dsRNA precursors of miRNAs and at least some 21- to 22-nt siRNAs are processed in the nucleus, the former bynuclear DCL1 and the latter by an unknown nuclear DCL.

TL;DR: A genetic screen to recover Arabidopsis mutants defective in RNA‐directed transcriptional silencing and methylation of a nopaline synthase promoter–neomycinphosphotransferase II (NOSpro–NPTII) target gene suggests that HDA6 is dispensable for RNA‐ directed de novo methylation, but necessary for reinforcing primarily C(N)G methylation induced by RNA.