Wilhelm G. Lachnit

TL;DR: P2X3 is critical for peripheral pain responses and afferent pathways controlling urinary bladder volume reflexes and may have therapeutic potential in the treatment of disorders of urine storage and voiding such as overactive bladder.

TL;DR: Polyclonal antisera generated to peptides corresponding to the carboxy termini of the seven cloned rat P2X receptors were compared using membranes from CHO‐K1 or 1321N1 cells expressing recombinant rat or human P2 X receptors to find useful reagents for the analysis of the P2x receptor subtypes in native tissues as well as for measuring receptor expression levels in transfected cell lines.

TL;DR: In this paper, the alpha 1-adrenoceptor population mediating contraction of caudal artery of rat has been characterized by using quantitative receptor pharmacology, and a selective alpha 1 A-adrienoceptor antagonist, A-61603, produced non-parallel, biphasic, dextral shifts of E/[A] curves to NA yielding a pKb of 8.9 (Schild regression slope = 1.0).

TL;DR: These results show for the first time, expression of a functional, homomeric recombinant rat P2X(3) receptor which is under regulated expression in a stably transfected mammalian cell line.

TL;DR: In this article, (±)-SDZ NVI 085 (3,4,4 a 5,10,10 a -hexahydro-6-methoxy-4-methyl-9-methylthio-2 H -naphth[2,3, b ]-1,4-oxazine hydrochloride), an α 1 -adrenoceptor agonist, produced a concentration-dependent relaxation (pIC 50 of 72 ± 01) in the isolated caudal artery of rat precontracted with serotonin (5-