William R. Alley

TL;DR: The focus of the review has been on glycoanalytical chemistry, which aims to isolate and structurally characterize biologically important glycoconjugates and synthesize carbohydrate structures for biochemical investigations, enabling technologies and medical applications and providing new therapeutics.

TL;DR: To characterize differences in total N-glycan levels, serum samples provided by 20 healthy control women were compared to those acquired from patients diagnosed with late-stage recurrent OC, and increased abundances of tri- and tetra-branched structures with varying degrees of sialylation and fucosylation were revealed in OC samples compared to controls.

TL;DR: The potential of electron-transfer dissociation (ETD) is exploited as a complementary approach for peptide fragmentation and allows not only the identification of the amino acid sequence of a glycopeptide, but also the unambiguous assignment of its glycosylation site.

TL;DR: Optimize LC gradients, together with nanospray MS, have been applied here in the quantitative measurements of N-linked glycans in blood serum, distinguishing breast cancer patients from control individuals.

TL;DR: A glycomic method is reported that selectively amidatesalpha2,6-linked sialic acids, while those that are alpha2,3-linked undergo spontaneous lactonization, which aids in increasing the metastatic potential of tumor cells.