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William R. Waud
Researcher at Duke University
Publications - 5
Citations - 782
William R. Waud is an academic researcher from Duke University. The author has contributed to research in topics: Xanthine dehydrogenase & Xanthine oxidase. The author has an hindex of 4, co-authored 5 publications receiving 776 citations.
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Journal ArticleDOI
A new purification procedure for bovine milk xanthine oxidase: effect of proteolysis on the subunit structure.
TL;DR: Pancreatinized enzyme has a greater mobility than unproteolyzed enzyme on polyacrylamide gels and is in good agreement with the minimum molecular weight of 157,000 calculated from dry weight determination and flavin analysis.
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The mechanism of conversion of rat liver xanthine dehydrogenase from an NAD+-dependent form (type D) to an O2-dependent form (type O).
William R. Waud,K.V. Rajagopalan +1 more
TL;DR: Rat liver xanthine dehydrogenase, type D, has been isolated directly from crude extracts as an antibody complex and its properties compared with those of two oxidase forms of the enzyme, heat- treated type O and trypsin-treated type O, also isolated as antibody complexes.
Journal ArticleDOI
Purification and properties of the NAD+-dependent (type D) and O2-dependent (type O) forms of rat liver xanthine dehydrogenase.
William R. Waud,K.V. Rajagopalan +1 more
TL;DR: The xanthine-oxidizing enzyme of rat liver has been purified as an NAD+-dependent dehydrogenase ( type D) and as the O2-dependent oxidase (type O).
Journal ArticleDOI
Inborn errors of molybdenum metabolism: combined deficiencies of sulfite oxidase and xanthine dehydrogenase in a patient lacking the molybdenum cofactor
Jean L. Johnson,William R. Waud,K.V. Rajagopalan,Marinus Duran,Frits A. Beemer,Sybe K. Wadman +5 more
TL;DR: A patient suffering from a combined deficiency of sulfite oxidase and xanthine dehydrogenase shows severe neurological abnormalities, dislocated ocular lenses, and mental retardation, and the metabolic defect responsible for loss of both enzyme activities appears to be at the level of the molybdenum cofactor common to the two enzymes.
Journal ArticleDOI
Combined deficiency of sulfite oxidase and xanthine oxidase as a result of defective synthesis of molybdenum-cofactor: 77
TL;DR: Investigations of Mo metabolism carried out suggested that a defective synthesis of Mo-cofactor causes the biochemical abnormalities and treatment with oral supplements of molybdenum did not result in biochemical or clinical improvement.