Journal ArticleDOI
A new purification procedure for bovine milk xanthine oxidase: effect of proteolysis on the subunit structure.
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TLDR
Pancreatinized enzyme has a greater mobility than unproteolyzed enzyme on polyacrylamide gels and is in good agreement with the minimum molecular weight of 157,000 calculated from dry weight determination and flavin analysis.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1975-08-01. It has received 229 citations till now. The article focuses on the topics: Xanthine oxidase & Polyacrylamide gel electrophoresis.read more
Citations
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Journal ArticleDOI
Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions
Wayne F. Beyer,Irwin Fridovich +1 more
TL;DR: The effects of experimental variables on assays based upon the use of either ferricytochrome c or nitro blue tetrazolium are investigated to help investigators to avoid the numerous potential pitfalls which necessarily surround these assay methods.
Journal ArticleDOI
Superoxide radical inhibits catalase.
Y Kono,Irwin Fridovich +1 more
TL;DR: In conclusion, catalase was inhibited by a flux of O2- generated in situ by the aerobic xanthine oxidase reaction, which provides the basis for a synergism between superoxide dismutase andCatalase.
Journal ArticleDOI
Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system
E W Kellogg rd,I Fridovich +1 more
TL;DR: The proposal that O2- and H2O2 can directly give rise to a singlet oxygen, as follows, was supported through the use of 2,5-dimethylfuran, as an indicating scavenger of singlets oxygen.
Journal ArticleDOI
The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme.
Ellen K. Hodgson,Irwin Fridovich +1 more
TL;DR: Bovine erythrocyte superoxide dismutase was slowly and irreversibly inactivated by hydrogen peroxide, and several compounds, including xanthine, urate, formate, and azide, protected the enzyme against inactivation by H2O2.
Book ChapterDOI
Preparation and assay of superoxide dismutases.
TL;DR: This chapter discusses the preparation and assay of superoxide dismutase (SOD), which is found in all oxygen-utilizing organisms and constitute a defense against oxygen toxicity.
References
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Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.
R.T. Swank,K.D. Munkres +1 more
TL;DR: Because of the importance of intrinsic charge and conformation, the system, although allowing a first approximation in molecular weight determination, may also be applicable to peptide “mapping,” particularly for “insoluble” peptide mixtures with prominent hydrophobic association, such as encountered in cellular membranes, viruses, and proteolytic digests.
Journal ArticleDOI
Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine).
TL;DR: The large molar absorptivity of 28,000 for the iron-ferrozine complex makes the ligand an attractive color reagent for monitoring iron therapy in anemic patients.
Journal ArticleDOI
An apparatus for preparative temperature-regulated polyacrylamide gel electrophoresis.
TL;DR: An apparatus suitable for preparative temperature-regulated polyacrylamide gel electrophoresis in discontinuous buffer systems is described and preliminary application to enzymes has demonstrated the feasibility of purification and recovery of activity.
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Superoxide Dismutase AN ENZYMIC FUNCTION FOR ERYTHROCUPREIN (HEMOCUPREIN)
Joe M. McCord,Irwin Fridovich +1 more