Fragmented or otherwise miniaturized plastic materials in the form of micro- or nanoplastics have been of nagging environmental concern. Perturbation of organismal physiology and behavior by micro- and nanoplastics have been widely documented for marine invertebrates. Some of these effects are also manifested by larger marine vertebrates such as fishes. More recently, possible effects of micro- and nanoplastics on mammalian gut microbiota as well as host cellular and metabolic toxicity have been reported in mouse models. Human exposure to micro- and nanoplastics occurs largely through ingestion, as these are found in food or derived from food packaging, but also in a less well-defined manner though inhalation. The pathophysiological consequences of acute and chronic micro- and nanoplastics exposure in the mammalian system, particularly humans, are yet unclear. In this review, we focus on the recent findings related to the potential toxicity and detrimental effects of micro- and nanoplastics as demonstrated in mouse models as well as human cell lines. The prevailing data suggest that micro- and nanoplastics accumulation in mammalian and human tissues would likely have negative, yet unclear long-term consequences. There is a need for cellular and systemic toxicity due to micro- and nanoplastics to be better illuminated, and the underlying mechanisms defined by further work.

Toxicity of Microplastics and Nanoplastics in Mammalian Systems

Aquaculture has made an enormous contribution to the world food production, especially to the sustainable supply of animal proteins. The utility of diverse reproduction strategies in fish, such as the exploiting use of unisexual gynogenesis, has created a typical case of fish genetic breeding. A number of fish species show substantial sexual dimorphism that is closely linked to multiple economic traits including growth rate and body size, and the efficient development of sex-linked genetic markers and sex control biotechnologies has provided significant approaches to increase the production and value for commercial purposes. Along with the rapid development of genomics and molecular genetic techniques, the genetic basis of sexual dimorphism has been gradually deciphered, and great progress has been made in the mechanisms of fish sex determination and identification of sex-determining genes. This review summarizes the progress to provide some directive and objective thinking for further research in this field.

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Genetic basis and biotechnological manipulation of sexual dimorphism and sex determination in fish

Gene Expression Patterns

Polyvinyl chloride microplastics induce growth inhibition and oxidative stress in Cyprinus carpio var. larvae.

Toxic effects on bioaccumulation, hematological parameters, oxidative stress, immune responses and neurotoxicity in fish exposed to microplastics: A review.

A novel male-specific DNA sequence in the common carp, Cyprinus carpio.

Toxic effects of imidacloprid on adult loach (Misgurnus anguillicaudatus).

Toxicity of imidazoles ionic liquid [C16mim]Cl to HepG2 cells.

Paramisgurnus dabryanus (Cypriniformes; Cobitidae), has been an emerging aquaculture species in China since the 1990s. In this study, random amplified polymorphic DNA fingerprinting with 220 primers was used to identify a sex-specific DNA marker in pooled DNA and individual DNA samples from male and female P. dabryanus. One primer, S2115, produced a novel sex-specific DNA fragment found only in tested females. This female-specific fragment was 917 bp with 36% GC content, and was named Pdff1. To further validate the authenticity of this female-specific marker for sexing, two PCR primers (pdff1-F and -R) were designed according to the cloned female-specific sequence. Amplification showed bands specific for females. Dot blot and Southern blot hybridization experiments both displayed female specificity using this marker as the probe. Two other P. dabryanus populations were tested by dot blot hybridization with the Pdff1 probe. The hybridization signals were seen in 33 or 43% of males in addition to all females in the Jinan and Xichuan populations, respectively. We propose to use this sex-specific marker to rapidly and specifically identify the gender of P. dabryanus from the ancient Yellow River Wetland in Yanjin, Henan Province. Our results could assist in cloning sex-specific chromosomal regions.

Xiaohua Xia

Papers

Polyvinyl chloride microplastics induce growth inhibition and oxidative stress in Cyprinus carpio var. larvae.

A novel male-specific DNA sequence in the common carp, Cyprinus carpio.

Toxic effects of imidacloprid on adult loach (Misgurnus anguillicaudatus).

Toxicity of imidazoles ionic liquid [C16mim]Cl to HepG2 cells.

Cloning and identification of a female-specific DNA marker in Paramisgurnus dabryanus