Xiaowo Wang

TL;DR: A protocol for the design, construction and expression of customized sgRNAs for transcriptional repression of any gene of interest, providing a complementary approach to RNA interference, which can be used in a wider variety of organisms.

TL;DR: It is found that NF-κB acts as a master regulator of the SASP, influencing the expression of more genes than RB and p53 combined, and a tumor-suppressive function of NF-σB that contributes to the outcome of cancer therapy is identified.

TL;DR: The data suggest that chromatin may exist in a markedly relaxed state after fertilization, followed by progressive maturation of higher-order chromatin architecture during early development, as characterized by slow consolidation of TADs and segregation of chromatin compartments in preimplantation development.

TL;DR: It is demonstrated that RB preferentially associates with E2F target genes involved in DNA replication and is uniquely required to repress these genes during senescence but not other growth states, which leads to RB loss leading to inappropriate DNA synthesis following a senescences trigger and enables extensive proliferation and rampant genomic instability.

TL;DR: Experiments show this approach has higher sensitivity and comparable specificity than other reported homologue searching methods and applied this method on Anopheles gambiae and detected 59 new miRNA genes.