L
Lei S. Qi
Researcher at Stanford University
Publications - 134
Citations - 24298
Lei S. Qi is an academic researcher from Stanford University. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 47, co-authored 106 publications receiving 19252 citations. Previous affiliations of Lei S. Qi include Lawrence Berkeley National Laboratory & University of California.
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Journal ArticleDOI
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.
Lei S. Qi,Matthew H. Larson,Luke A. Gilbert,Jennifer A. Doudna,Jonathan S. Weissman,Adam P. Arkin,Adam P. Arkin,Wendell A. Lim +7 more
TL;DR: This RNA-guided DNA recognition platform provides a simple approach for selectively perturbing gene expression on a genome-wide scale and can efficiently repress expression of targeted genes in Escherichia coli, with no detectable off-target effects.
Journal ArticleDOI
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Luke A. Gilbert,Matthew H. Larson,Leonardo Morsut,Zairan Liu,Gloria A. Brar,Sandra Elizabeth Torres,Noam Stern-Ginossar,Onn Brandman,Evan H. Whitehead,Jennifer A. Doudna,Wendell A. Lim,Jonathan S. Weissman,Lei S. Qi +12 more
TL;DR: The results establish that the CRISPR system can be used as a modular and flexible DNA-binding platform for the recruitment of proteins to a target DNA sequence, revealing the potential of CRISpri as a general tool for the precise regulation of gene expression in eukaryotic cells.
Journal ArticleDOI
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Luke A. Gilbert,Max A. Horlbeck,Britt Adamson,Jacqueline E. Villalta,Yuwen Chen,Evan H. Whitehead,Carla P. Guimaraes,Barbara Panning,Hidde L. Ploegh,Michael C. Bassik,Lei S. Qi,Martin Kampmann,Jonathan S. Weissman +12 more
TL;DR: This work identifies rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90%-99% knockdown with minimal off-target effects, and activators to endogenous genes via endonuclease-deficient Cas9, which enable modulation of gene expression over a ∼1,000-fold range.
Journal ArticleDOI
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
Baohui Chen,Luke A. Gilbert,Beth A. Cimini,Joerg Schnitzbauer,Wei Zhang,Gene-Wei Li,Jason Park,Elizabeth H. Blackburn,Jonathan S. Weissman,Lei S. Qi,Bo Huang +10 more
TL;DR: Using an EGFP-tagged endonuclease-deficient Cas9 protein and a structurally optimized small guide (sg) RNA, robust imaging of repetitive elements in telomeres and coding genes in living cells is demonstrated by repurposing the bacterial CRISPR/Cas system.
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
Luke A. Gilbert,Max A. Horlbeck,Britt Adamson,Jacqueline E. Villalta,Yuwen Chen,Evan H. Whitehead,Carla P. Guimaraes,Barbara Panning,Michael C. Bassik,Lei S. Qi,Martin Kampmann,Jonathan S. Weissman,Hidde L. Ploegh +12 more
TL;DR: In this article, the authors identify rules for specific targeting of transcriptional repressors (CRISPRi), typically achieving 90%-99% knockdown with minimal off-target effects, and activators (CRisPRa) to endogenous genes via endonuclease-deficient Cas9.