Qualified presumption of safety (QPS) was developed to provide a generic safety evaluation for biological agents to support EFSA's Scientific Panels. The taxonomic identity, body of knowledge, safety concerns and antimicrobial resistance are assessed. Safety concerns identified for a taxonomic unit (TU) are where possible to be confirmed at strain or product level, reflected by 'qualifications'. No new information was found that would change the previously recommended QPS TUs and their qualifications. The list of microorganisms notified to EFSA was updated with 54 biological agents, received between April and September 2019; 23 already had QPS status, 14 were excluded from the QPS exercise (7 filamentous fungi, 6 Escherichia coli, Sphingomonas paucimobilis which was already evaluated). Seventeen, corresponding to 16 TUs, were evaluated for possible QPS status, fourteen of these for the first time, and Protaminobacter rubrum, evaluated previously, was excluded because it is not a valid species. Eight TUs are recommended for QPS status. Lactobacillus parafarraginis and Zygosaccharomyces rouxii are recommended to be included in the QPS list. Parageobacillus thermoglucosidasius and Paenibacillus illinoisensis can be recommended for the QPS list with the qualification 'for production purposes only' and absence of toxigenic potential. Bacillus velezensis can be recommended for the QPS list with the qualification 'absence of toxigenic potential and the absence of aminoglycoside production ability'. Cupriavidus necator, Aurantiochytrium limacinum and Tetraselmis chuii can be recommended for the QPS list with the qualification 'production purposes only'. Pantoea ananatis is not recommended for the QPS list due to lack of body of knowledge in relation to its pathogenicity potential for plants. Corynebacterium stationis, Hamamotoa singularis, Rhodococcus aetherivorans and Rhodococcus ruber cannot be recommended for the QPS list due to lack of body of knowledge. Kodamaea ohmeri cannot be recommended for the QPS list due to safety concerns.

https://efsa.onlinelibrary.wiley.com/doi/pdf/10.2903/j.efsa.2020.5965

Update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA 13: suitability of taxonomic units notified to EFSA until September 2020.

Toxic effects of lead exposure on bioaccumulation, oxidative stress, neurotoxicity, and immune responses in fish: A review

The use of Bacillus species in maintenance of water quality in aquaculture: A review

In our daily life, we are surrounded by harmful pollutants, including heavy metals that are not visible in the macroscopic view easily. Heavy metals can disrupt different aspects of human health, such as the immune system which has gained a lot of attention in recent decades. This had led to its rapid progression and new insights into its alterations in different diseases especially cancer. Heavy metals are non-biodegradable materials that exist in different parts of the food cycle, such as fruits and vegetables as commonly consumed foods and also unexpected sources such as street dust, that exists in the streets that we pass every day, soil, air, and water. These heavy metals can enter the human body through respiratory, cutaneous, and gastrointestinal pathways and then accumulate in different organs, leading to their encountering with various parts of the body. These sources and natural characteristics of heavy metals facilitate their interaction with the immune system. In this review, we investigated the effect of lead and cadmium, as pollutants that exist in many different parts of the human environment, on the immune system which is known to have a key role in the pathophysiology of cancer.

Effects of lead and cadmium on the immune system and cancer progression.

Thiamethoxam (TMX) is a widely used neonicotinoid insecticide for its effective potential for controlling insects from the agricultural field, which might induce toxicity to the aquatic biota. In this study, the role of the probiotic Bacillus subtilis (BS) and a phytogenic oil extract of Thymus vulgaris essential oil (TVEO) in the modulation of thiamethoxam (TMX)-induced hepatorenal damage, oxidative stress, and immunotoxicity in African catfish (Clarias garipenus) has been evaluated. Fish were subjected to TMX (5 mg L−1) and fed with a diet either supplemented with BS (1000 ppm) or TVEO (500 ppm). The experiment lasted for 1 month. By the end of the experiment, blood was sampled for biochemical analysis and fish organs and tissues were collected for histopathological and immunohistochemical examinations. Results showed a substantial increase of serum markers of hepatorenal damage such as the activities of aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) and levels of blood urea nitrogen (BUN) and creatinine with an obvious decrease of serum protein levels in the TMX-intoxicated group. Also, there was a considerable increase in malondialdehyde (MDA) levels and glutathione-S-transferase (GST) activity. TMX remarkably suppressed serum lysozyme activity, respiratory burst activity, and phagocytosis with a conspicuous elevation of the levels of interleukins (interleukin-1 beta (IL-1β) and interleukin-6 IL-6). The histopathological findings showed that TMX induced degenerative changes and necrosis in the gills, liver, head kidneys, and spleen of the intoxicated fish. Significant alterations of frequency, size, and area percentage of melanomacrophage centers (MMCs), decreased splenocyte proliferation, and increased number of caspase-3 immunopositive cells were also observed. Contrariwise, the concurrent supplementation of either BS or TVEO in the diets of catfish partially mitigated both the histopathological and histomorphometric lesions of the examined tissues. Correspondingly, they improved the counts of proliferating cell nuclear antigen (PCNA) and caspase-3 immunopositive splenocytes. In conclusion, the co-administration of either BS or TVEO in catfish diets partially diminished the toxic impacts of TMX. Nonetheless, the inclusion of TVEO in the diets of catfish elicited better protection than BS against TMX-induced toxicity in response to its potential anti-inflammatory, antioxidant, anti-apoptotic, and immune-stimulant effects.

Modulatory role of dietary Thymus vulgaris essential oil and Bacillus subtilis against thiamethoxam-induced hepatorenal damage, oxidative stress, and immunotoxicity in African catfish (Clarias garipenus)

Effect of sub-chronic exposure to lead (Pb) and Bacillus subtilis on Carassius auratus gibelio: Bioaccumulation, antioxidant responses and immune responses.

The aim of this study was to explore the effects of lead exposure on the antioxidant and immune responses of Crucian carp. Three hundred sixty healthy Crucian carp were randomly grouped into four groups and exposed to different doses of lead (0, 0.05, 0.5, and 1 mg/L). Fish were sampled at 30 and 60 days, respectively, and antioxidant capability, immune parameters, ALAD activity, and immune-related genes were assessed. The results showed that T-AOC and GSH activities of the liver were significantly increased in 60 days (P < 0.05); the activities of SOD, CAT, T-AOC, and GSH were significantly increased (P < 0.05) compared to the control in the kidney in 60 days. With an increase in Pb dose, the activity and expression of lysozyme and the content of immunoglobulin M were significantly decreased compared to the control. Compared with the control group, the activity of ALAD in the lead-exposed group decreased significantly (P < 0.05). The expression of the HSP70, tumor necrosis factor-α (TNF-α), interleukins (IL-10), and immunoglobulin M genes was all enhanced in lead-exposed group, whereas lysozyme gene expression was decreased. The results indicated that lead induced oxidative stress and had immunotoxic effects on Crucian carp.

Effect of Lead on Antioxidant Ability and Immune Responses of Crucian Carp.

The present study aimed to identify differentially expressed genes (DEGs) and major signal transduction pathways that were related to the immune response of epithelioma papulosum cyprinid (EPC) cells to reoviruses isolated from allogynogenetic silver crucian carp. The study also lays a theoretical foundation for the pathogenesis and immunity of the reovirus, which is helpful to the breeding of cyprinids fish. Reovirus infected and uninfected EPC cells were analyzed by using a new-generation high-throughput sequencing technology. DEGs were identified, annotated, and classified, and the signal pathways involved in the response to reovirus infection were identified by using bioinformatics tool. The data were assembled into 92,101 contigs with an average length of 835.24 bp and an N50 value of 1432 nt. Differential expression analysis of all the genes identified 3316 DEGs at a false discovery rate (FDR) of <0.01 and a fold-change of ≥3, of which 1691 were upregulated genes, 1625 were downregulated, and about 305 were immune-related genes. Gene Ontology (GO) enrichment analysis resulted in the annotation of 3941 GO terms, including 2719 biological processes (37,810 unigenes), 376 cell components (7943 unigenes), and 846 molecular functions (11,750 unigenes). KEGG metabolic pathway analysis matched the DEGs from pre-and post-infection EPC cells to 193 pathways, of which 35 were immune-related, including the Toll-like receptor, cytokine-cytokine receptor interaction, and the JAK-STAT signaling pathways.

Xiaoyan Du

Papers

Effect of sub-chronic exposure to lead (Pb) and Bacillus subtilis on Carassius auratus gibelio: Bioaccumulation, antioxidant responses and immune responses.

Effect of Lead on Antioxidant Ability and Immune Responses of Crucian Carp.

Transcriptome Analysis of Epithelioma Papulosum Cyprini Cells Infected by Reovirus Isolated from Allogynogenetic Silver Crucian Carp.