The invention belongs to the field of genetic engineering, and in particular relates to an aspergillus cristatus feruloyl esterase gene as well as engineering bacteria and application thereof. The nucleotide sequence of the aspergillus cristatus feruloyl esterase gene is shown in SEQ ID No. 1. Feruloyl esterase encoded by the aspergillus cristatus feruloyl esterase gene has an amino acid sequenceshown in SEQ ID No. 2. The aspergillus cristatus feruloyl esterase gene provided by the invention can be used for in vitro generation of ferulic acid. Tests prove that the release rate of the ferulicacid in de-starch wheat bran suspension is 75.3%; the aspergillus cristatus feruloyl esterase gene enables the release rate of the ferulic acid to be much higher than other feruloyl esterase genes; therefore, a new enzyme source is provided for production of ferulic acid.

Xue Jiajun

Papers

Aspergillus cristatus feruloyl esterase gene as well as engineering bacteria and application of thereof