다중혈관 관상동맥 환자에서 y-문합을 이용하여 양쪽 내흉동맥만을 사용한 우회술의 조기 성적

[신간의 별자리x] 우리/미술, 그리고 ‘슬픔의 박물관’

Future cell-based therapies such as tissue engineering will benefit from a source of autologous pluripotent stem cells. For mesodermal tissue engineering, one such source of cells is the bone marrow stroma. The bone marrow compartment contains several cell populations, including mesenchymal stem cells (MSCs) that are capable of differentiating into adipogenic, osteogenic, chondrogenic, and myogenic cells. However, autologous bone marrow procurement has potential limitations. An alternate source of autologous adult stem cells that is obtainable in large quantities, under local anesthesia, with minimal discomfort would be advantageous. In this study, we determined if a population of stem cells could be isolated from human adipose tissue. Human adipose tissue, obtained by suction-assisted lipectomy (i.e., liposuction), was processed to obtain a fibroblast-like population of cells or a processed lipoaspirate (PLA). These PLA cells can be maintained in vitro for extended periods with stable population doubling and low levels of senescence. Immunofluorescence and flow cytometry show that the majority of PLA cells are of mesodermal or mesenchymal origin with low levels of contaminating pericytes, endothelial cells, and smooth muscle cells. Finally, PLA cells differentiate in vitro into adipogenic, chondrogenic, myogenic, and osteogenic cells in the presence of lineage-specific induction factors. In conclusion, the data support the hypothesis that a human lipoaspirate contains multipotent cells and may represent an alternative stem cell source to bone marrow-derived MSCs.

/pdf/multilineage-cells-from-human-adipose-tissue-implications-4z32vwpf0q.pdf

Multilineage cells from human adipose tissue: implications for cell-based therapies.

https://www.researchgate.net/file.PostFileLoader.html?id=526892a1d4c118a61d4360bd&assetKey=AS%3A272155479085058%401441898330516

Porosity of 3D biomaterial scaffolds and osteogenesis.

Hydrogels for tissue engineering: scaffold design variables and applications.

Intra-articular administration of gelatin hydrogels incorporating rapamycin-micelle reduces development of experimental osteoarthritis in a murine model

Recent development of biomedical engineering as well as basic biology and medicine has enabled us to induce cell-based regeneration of body tissue assisted with the self-repairing potential tissue or substitute biological functions of damaged organs with cells. For successful tissue regeneration, it is indispensable to give cells an environment suitable for induction of cell-based tissue regeneration. Tissue engineering is a newly emerging biomedical technology to create the environment for tissue regeneration with various biomaterials. This paper overviews recent researches and clinical data about oral tissues regeneration based on tissue engineering briefly explaining the key technologies of tissue engineering.

/pdf/recent-advances-in-tissue-engineering-for-regeneration-of-ydoe62ftrc.pdf

Recent advances in tissue engineering for regeneration of oral tissues

regeneration of tracheal cartilage in a canine model Slow release of bone morphogenetic protein 2 from a gelatin sponge to promote

Corneal Endothelial Wound Healing Is Promoted by the Injection of bFGF-Impregnated Gelatin Microspheres in Rabbit Cryo-Injury Model

We previously reported functionally graded hydroxyapatite (fg-HAp) with the characteristics of blood permeability into the bulk and osteoinduction by adding low dose of bone morphogenetic protein-2 (BMP-2). In this study, we evaluated the bioactivity of the obtained dense HAp bodies in a simulated body fluid (SBF) and the osteoinductive activity with or without SBF treatment in vivo. The fg-HApSBF was prepared by immersing the fg-HAp in the SBF solution at 14 days and the surface structure was observed by SEM. The fg-HApSBF showed fine bone-like crystal on the surface of the HAp. Ectopic bone formation occurred in the fg-HAp/BMP-2 (1.0, 0.5μg) system at 3 weeks, while only in the fg-HApSBF/BMP-2 (1.0μg), bone induction was found. The histological finding showed body fluid permeation into the fg-HApSBF bulk and bone formation were obserbed.

Yasuhiko Tabata

Papers

Intra-articular administration of gelatin hydrogels incorporating rapamycin-micelle reduces development of experimental osteoarthritis in a murine model

Recent advances in tissue engineering for regeneration of oral tissues

regeneration of tracheal cartilage in a canine model Slow release of bone morphogenetic protein 2 from a gelatin sponge to promote

Corneal Endothelial Wound Healing Is Promoted by the Injection of bFGF-Impregnated Gelatin Microspheres in Rabbit Cryo-Injury Model

Characteristics of Surface Behavior and Osteoinductivity of Biomimetic Ceramic Scaffold