Yejun Zou

TL;DR: This study developed an intensely fluorescent, rapidly responsive, pH-resistant, genetically encoded sensor of wide dynamic range, denoted SoNar, for tracking cytosolic NAD(+) and NADH redox states in living cells and in vivo, and identified KP372-1 as a potent NQO1-mediated redox cycling agent that produced extreme oxidative stress, selectively induced cancer cell apoptosis, and effectively decreased tumor growth in vivo.

TL;DR: It is found that mammalian cells have a strong tendency to maintain physiological NADPH homeostasis, which is regulated by glucose-6-phosphate dehydrogenase and AMP kinase, and using the iNap sensors, NADPH fluctuations during the activation of macrophage cells or wound response in vivo are monitored.

TL;DR: A detailed protocol to image and monitor NAD+/NADH redox state in living cells and in vivo using a highly responsive, genetically encoded fluorescent sensor known as SoNar (sensor of NAD(H) redox).

TL;DR: In this article, a series of Ni-Fe-B catalysts with different Fe/(Fe + Ni) molar ratios were used for the hydrolysis of NaBH4.

TL;DR: This protocol describes how to combine up to four genetically encoded fluorescent sensors to image redox landscapes and describes applications in live imaging and flow cytometry of cultured cells, and in vivo imaging in zebrafish larvae.