Yuzheng Zhao

TL;DR: This study developed an intensely fluorescent, rapidly responsive, pH-resistant, genetically encoded sensor of wide dynamic range, denoted SoNar, for tracking cytosolic NAD(+) and NADH redox states in living cells and in vivo, and identified KP372-1 as a potent NQO1-mediated redox cycling agent that produced extreme oxidative stress, selectively induced cancer cell apoptosis, and effectively decreased tumor growth in vivo.

TL;DR: The results support the view that cytosolic NADH is sensitive to environmental changes, while mitochondria have a strong tendency to maintain physiological NADH homeostasis.

TL;DR: This study uncovers a previously unknown mechanism by which acetylation negatively regulates G6PD activity to maintain cellular NADPH homeostasis during oxidative stress.

TL;DR: It is found that mammalian cells have a strong tendency to maintain physiological NADPH homeostasis, which is regulated by glucose-6-phosphate dehydrogenase and AMP kinase, and using the iNap sensors, NADPH fluctuations during the activation of macrophage cells or wound response in vivo are monitored.

TL;DR: Peppers, a series of monomeric, bright and stable FRs with a broad range of emission maxima spanning from cyan to red, are developed and it is believed these FRs will be useful tools for live imaging of cellular RNAs.