Yibing Liu

TL;DR: A positive role of glucose in the improvement of sperm cryopreservation in farmed greenlip abalone is demonstrated, showing that the addition of glucose could significantly improve the sperm plasma membrane integrity and mitochondrial membrane potential.

TL;DR: The latest developments in marine bivalves and gastropods sperm cryopreservation were reviewed and summarized for seven key steps: sperm collection, extender selection, cryoprotectant agent selection, cooling rate, thawing temperature, and quality assessment.

TL;DR: Investigation on monosaccharides demonstrated that glucose, fructose and galactose had a similar cryoprotective effect, resulting in a similar level of the post-thaw sperm fertilization rate, plasma membrane integrity, mitochondrial membrane potential and acrosome integrity.

TL;DR: This study developed a technique of sperm cryopreservation using liquid nitrogen (LN) vapour in farmed blacklip abalone Haliotis rubra through evaluating the following five key factors: cryoprotectant agent (CPA) toxicity; cooling temperature; thawing temperature; sperm to egg ratio and sugar addition, using sperm motility or fertilization rate as quality assessment indicators.

TL;DR: The larval cryopreservation technique developed in this study would enhance the breeding program and all year-round hatchery production in Mytilus galloprovinvialis.