Showing papers by "Yoo Gyeong Park published in 2014"

Physiological and Proteomic Analysis in Chloroplasts of Solanum lycopersicum L. under Silicon Efficiency and Salinity Stress

Abstract: Tomato plants often grow in saline environments in Mediterranean countries where salt accumulation in the soil is a major abiotic stress that limits its productivity However, silicon (Si) supplementation has been reported to improve tolerance against several forms of abiotic stress The primary aim of our study was to investigate, using comparative physiological and proteomic approaches, salinity stress in chloroplasts of tomato under silicon supplementation Tomato seedlings (Solanum lycopersicum L) were grown in nutrient media in the presence or absence of NaCl and supplemented with silicon for 5 days Salinity stress caused oxidative damage, followed by a decrease in silicon concentrations in the leaves of the tomato plants However, supplementation with silicon had an overall protective effect against this stress The major physiological parameters measured in our studies including total chlorophyll and carotenoid content were largely decreased under salinity stress, but were recovered in the presence of silicon Insufficient levels of net-photosynthesis, transpiration and stomatal conductance were also largely improved by silicon supplementation Proteomics analysis of chloroplasts analyzed by 2D-BN-PAGE (second-dimensional blue native polyacrylamide-gel electrophoresis) revealed a high sensitivity of multiprotein complex proteins (MCPs) such as photosystems I (PSI) and II (PSII) to the presence of saline A significant reduction in cytochrome b6/f and the ATP-synthase complex was also alleviated by silicon during salinity stress, while the complex forms of light harvesting complex trimers and monomers (LHCs) were rapidly up-regulated Our results suggest that silicon plays an important role in moderating damage to chloroplasts and their metabolism in saline environments We therefore hypothesize that tomato plants have a greater capacity for tolerating saline stress through the improvement of photosynthetic metabolism and chloroplast proteome expression after silicon supplementation

Changes in Content of Total Polyphenol and Activities of Antioxidizing Enzymes in Perilla frutescens var. acuta Kudo and Salvia plebeia R. Br. as Affected by Light Intensity

Abstract: The effect of light intensity on content of total polyphenol and activities of antioxidizing enzymes of P. frutescens var. acuta Kudo (Perilla) and S. plebeia R. Br. (Salvia) under a controlled environment was investigated. The three treatments used were T1, 300 μmol·m-2·s-1 PPFD for 38 days; T2, 300 μmol·m-2·s-1 PPFD for 31 days and 500 μmol·m-2·s-1 PPFD for additional 7 days; and T3, 500 μmol·m-2·s-1 PPFD for 38 days. After 38 days of cultivation, shoot and root growth, contents of total chlorophyll, anthocyanin and total polyphenol, and activities of antioxidizing enzymes were measured. The shoot and root growth decreased in the T2 and T3 treatments. Moreover, the incidence of tip burn increased in the T3 treatment in both species. Anthocyanin content in the Perilla decreased, while total chlorophyll content of the Salvia increased, in the T2 and T3 treatments as compared to the T1 treatment. Total polyphenol content and activities of antioxidizing enzymes increased in the Perilla in the T3 and in the Salvia in the T2 treatments.

Growth and quality of plug seedlings of three indigenous medicinal plants as affected by ionic strength of the nutrient solution

Abstract: The objective of this study was to investigate the effect of ionic strength of the nutrient solution on growth and quality of plug seedlings of three indigenous medicinal plant species. Seeds were sown in 200-cell plug trays, containing a commercial medium in a glasshouse. The pH of a nutrient solution supplied was adjusted to 5.96. The electrical conductivities (EC) of the nutrient solutions supplied were 0.43 dS·m−1 (1/4x ionic strength), 0.84 dS·m−1 (1/2x ionic strength), and 1.20 dS·m−1 (1x ionic strength). Growth and quality of P. frutescens var. acuta Kudo and A. gigas Nakai were the greatest in the 1/2x ionic strength, while those of S. tonkinensis were the greatest in the 1x ionic strength. Elemental contents in the shoot and root of P. frutescens var. acuta Kudo were the greatest in the 1/2x ionic strength, while those of S. tonkinensis were the least in the 1/2x ionic strength and of A. gigas Nakai were the greatest in the 1x ionic strength. Effect of ionic strength of the nutrient solution on plant growth was not the same in all species, but slightly different depending on the plant species. Seedlings applied with a solution with a proper ionic strength grew faster and were of better quality.

Effect of Cell Size on Growth and Development of Plug Seedlings of Three Indigenous Medicinal Plants

Abstract: There have not been many studies conducted on the seedling production, especially in plug trays, of traditional medicinal plant species In an effort to establish guide lines for seedling production, this study investigated the effect of plug cell size on the growth and development of plug seedling of three medicinal plant species Seeds were sown in either 128, 200, or 288-cell plug trays, containing a commercial medium Growth and development of individual seedling was generally promoted with increasing size of a plug cell in all of the three species The greatest biomass of the seedlings gained in a plug tray was obtained in the 288-cell trays in Perilla frutescens var acuta Kudo and Sophora tonkinensis, and the 200-cell trays in Angelica gigas Nakai Overall growth and development of the shoot and root of a single seedling of Perilla frutescens var acuta Kudo, except total chlorophyll and anthocyanin contents, was the greatest in the 128-cell tray However, length of the longest root, length, width and area of the leaf, internode length, root fresh weight, and root ball formation in the 200- and 288-cell trays were not significantly different each other In Sophora tonkinensis, although length of the longest root, stem diameter, leaf width, leaf area, shoot fresh weight, and root ball formation were not significantly different among the treatments, length of the longest root and root ball formation of a single seedling were the greatest in the 128-cell tray Overall shoot and root growth, except total chlorophyll content, of a single seedling of Angelica gigas Nakai was the greatest in the 128-cell tray Based on the total biomass, it is concluded that 288-cell trays are recommended for production of plug seedlings of medicinal plant species P frutescens var acuta Kudo and S tonkinensis In A gigas Nakai, it would be more economical to use the 200-cell trays than 128-cell trays due to total biomass

Flower yield and quality of two rose cultivars grown in phenolic foam LC slab and phenolic foam RC slab in comparison to perlite and rockwool slab

Abstract: Two standard cut rose (Rosa hybrida) ‘Rock Fire’ and ‘Feel Lip’ were propagated by single nodal cuttings. Each cutting with a five-leaflet leaf was stuck in a rockwool cube (Delta, 5 cm × 5 cm × 5 cm, Grodan Co., Ltd., Denmark) on May 16, 2011. After 32 days, rooted uniform saplings were transplanted onto the four different growing media, perlite (Pergreen, No. 1, G-Biotech Co., Ltd., Korea), rockwool slab (Delta, 100 cm × 20 cm × 7.5 cm, Grodan Co. Ltd., Denmark), phenolic foam LC slab (100 cm × 20 cm × 7.5 cm, Smithers Oasis Co., Ltd., Korea), or phenolic foam RC slab (100 cm × 20 cm × 7.5 cm, Smithers Oasis Co., Ltd., Korea), laid out in a completely randomized design on a bench in a commercial grower’s plastic house. The nutrient solution was supplied uniformly through a drip irrigation system. The irrigation frequency was dependent on amount of solar radiation. Yield and quality of harvested flower stems were measured for five successive harvests during a period of 11 months. Growth parameters measured were stem length, stem diameter, number of five-leaflet leaves per stem, stem fresh weight, number of harvested cut flowers per ten plants by grade, total yield, and tissue contents of inorganic elements. Measured growth parameters, such as stem length, number and quality of harvested stems, and contents of inorganic elements, were similar in all the tested growing media, except perlite in which these values were the lowest. The results obtained suggests that the widely used rockwool slab can be replaced with the new phenolic medium such as phenolic foam LC slab for commercial scale hydroponic productions of cut roses.