Yoshio Tominaga

TL;DR: In this paper, a novel lipase characterized in that at least 95% of its original activity is maintained after being treated at a temperature of 30° to 80° C. for 15 minutes, which may be produced by cultivating Rhizopus chinensis FERM BP-936 in a culture medium and recovering the produced enzyme from the medium.

TL;DR: The enzyme showed lower activities towards ester bonds of polyunsaturated fatty acids as compared with those of other acids, and was proved effective for increasing the concentration of docosahexaenoic acid in tuna oil.

Abstract: Glycosides, the reducing ends of which were glycerol and 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (trimethylolpropane, TMP), were esterified with oleic acid (OA) by a lipase from Candida cylindracea. Two glycosides, 1-O-β-d-galactosyl glycerol (Gal-1-Gl) and O-β-d-glucosyl TMP (Glc-TMP), were enzymatically synthesized by transglycosylation. GIc-TMP was esterified at a higher water content than Gal-1-Gl. When an equal amount of the glycosides and OA were used as substrates, almost 20% and 50% of OA were consumed for the esterification of Gal-1-Gl and Glc-TMP, respectively. Laurie, capric, and caprylic acids also gave esters. Oleic ester synthesis was compared among several related glycosides. The enzyme esterified the glycosides with a TMP moiety better than those with a glycerol moiety. The reaction was scarcely affected not only by the kind of monosaccharide moieties but also by anomeric linkage types between the moieties.

TL;DR: Thermostable lipases are useful for their industrial application and the increase in thermostability of 1,3-positionally specific lipases from Fusarium heterosporum and Rhizopus oryzae is focused on.