Yunsoo Yeo

TL;DR: These yeast now provide a convenient format for investigating catalytic coupling between terpene synthases and hydroxylases, as well as a platform for the industrial production of high value, single‐entity and stereochemically unique terpenes.

TL;DR: In this paper, a de novo transcriptome assembly was developed for Deadly Nightshade (Atropa belladonna), and mining of the transcriptome identified a phylogenetically distinct aromatic amino acid aminotransferase (ArAT), designated Ab-ArAT4, that is coexpressed with known tropane alkaloid biosynthesis genes in the roots of A.belladonna.

TL;DR: This work has shown that through the sequencing of even a few cDNA libraries, a broad representation of the transcriptome of any medicinal plant species can be obtained, providing a robust resource for gene discovery and downstream biochemical pathway discovery.

TL;DR: Isolated and purified nucleic acid molecules encoding Hyoscyamus muticus premnaspirodiene oxidase (HPO) have been prepared by gene cloning as discussed by the authors.

TL;DR: This chapter discusses that molecular biologists and geneticists are determining mechanisms regulating the expression of this biosynthetic machinery, and biochemists are elucidating structure–function relationships for key enzymes in the pathway.