Showing papers by "Gifu University of Medical Science published in 2014"

Oral vaccination with a liposome‐encapsulated influenza DNA vaccine protects mice against respiratory challenge infection

Abstract: It is well accepted that vaccination by oral administration has many advantages over injected parenteral immunization. The present study focuses on whether oral vaccination with a DNA vaccine could induce protective immunity against respiratory challenge infection. The M1 gene of influenza A virus was used to construct DNA vaccine using pcDNA 3.1(+) plasmid, a eukaryotic expression vector. The cationic liposomes were used to deliver the constructed DNA vaccine. In vitro and in vivo expression of M1 gene was observed in the cell line and in the intestine of orally vaccinated C57BL/6 mice, respectively. It became clear that this type of oral DNA vaccination was capable of inducing both humoral and cellular immune responses, together with an augmentation of IFN-γ production. In addition, oral vaccination with liposome-encapsulated DNA vaccine could protect the mice against respiratory challenge infection. These results suggest that gastrointestinal tract, a constituent member of the common mucosal immune system, is a potent candidate applicable as a DNA vaccine route against virus respiratory diseases.

RR interval variability during galvanic vestibular stimulation correlates with arterial pressure upon head-up tilt

Abstract: RR interval variability (RRIV) in the supine position without and with galvanic vestibular stimulation (GVS (off) and GVS (on), respectively), changes in mean arterial pressure (MAP) at the onset of 60° head-up tilt (HUT) during GVS (off), and their relationship were analyzed in 25 healthy young subjects. MAP decreased by less than 5mmHg or increased upon HUT in 12 subjects (UP), but MAP decreased by more than 5mmHg in 13 subjects (DOWN). Applying sinusoidal GVS of 2mA at a random frequency of 0.2 to 10.0Hz did not change the RR intervals or MAP. However, the high frequency component (HF) of RRIV increased in both UP and DOWN subjects. The increase in DOWN subjects was larger than that in UP subjects. The ratio of the low frequency component to HF (L/H) increased in UP subjects during GVS (on), but did not reach a significant level in DOWN subjects. The changes in the HF were significantly correlated with changes in MAP at the onset of HUT; i.e., the subjects with larger increases in the HF during GVS (on) showed larger decreases in MAP. Thus, GVS or vestibular input during HUT possibly activates the vagal nerves, and the dominance of excitation in sympathetic or vagal nerves during vestibular stimulation is important for controlling MAP at the onset of HUT.

Compression stocking length effects on arterial blood pressure and heart rate following head-up tilt in healthy volunteers.

Abstract: Background: Change in posture from supine to standing induces a footward fluid shift. This shift might decrease arterial pressure (AP) and induce orthostatic hypotension. To prevent decrease in AP, compression stockings are recommended, but the effects of various lengths have not been compared. Objective: The aim was to compare AP and heart rate (HR) by compression stocking length during head-up tilt (HUT). Methods: The effects of elastic compression stockings of three lengths on AP and HR were examined at the onset of HUT. The stockings were knee-high, compressing the foot and calf; thigh-high, compressing the toe to the thigh; and waist-high, compressing the toe to the waist. Results: AP did not significantly change at the onset of HUT without stockings or with any length of stockings compared with the AP in the supine position. HR was significantly higher at the onset of HUT without stockings and with the knee-high and thigh-high stockings than that during supine position. No significant difference in HR was observed among the three conditions. However, the HR increase was significantly depressed with the waist-high stockings. Discussion: These results suggest that the use of waist-high stockings that afford abdominal compression is needed to affect cardiovascular dynamics at the onset of HUT and may prevent orthostatic hypotension.

Infection with respiratory syncytial virus influences FasL-mediated apoptosis of pulmonary γδ T cells in a murine model of allergen sensitization.

Abstract: Background: It has been reported that adoptive transfer of γδ T cells increases the cellular infiltration, especially eosinophils, in the lungs of allergic mice, suggesting that γδ T cells may play a proinflammatory role in allergic airway inflammation. Respiratory syncytial virus (RSV) infection can decrease the number of Th2-type γδ T cells. However, the underlying mechanisms remain unknown. Methods: BALB/c mice were inoculated intranasally with RSV before or after sensitization to OVA. The amounts of Th1/Th2 cytokines as well as the levels of specific antibodies were determined by ELISA. The apoptotic death of pulmonary γδ T cells was analyzed by flow cytometry. Results: Adoptive transfer of γδ T cells increased the production of Th2 cytokines in the lungs and allergy-related antibodies in the serum, further confirming that γδ T cells act as pro-inflammatory cells or a promoter for the development of allergic asthma. RSV infection before sensitization to OVA enhanced apoptotic death of pulmonar...

Myoclonic atonic epilepsy: Another generalized epilepsy syndrome that is “not so” generalized

Abstract: Myoclonic atonic/astatic epilepsy (MAE), first described well by Doose1 (pronounced dough sah: ), is a generalized electroclinical syndrome with early onset characterized by myoclonic, atonic/astatic, generalized tonic-clonic, and absence seizures (but not tonic seizures) in association with generalized spike-wave (GSW) discharges. Thought to have a genetic component that has proven to be complicated,2 MAE sometimes occurs in children who have otherwise been developing normally and has variable outcome. MAE is typically treated with antiseizure medications that are used for generalized epilepsy syndromes, with perhaps a best response to valproate, felbamate, or the ketogenic diet.3,4

Reconstruction of semiautomated cardiac regions of interest using iodine-123 metaiodobenzylguanidine myocardial scintigraphy.

Abstract: INTRODUCTION Analysis using cardiac iodine-123 metaiodobenzylguanidine (MIBG) scintigraphy with regions of interest (ROIs) is useful for assessing myocardial sympathetic activity. However, manual placement of the cardiac ROI is sometimes difficult because myocardial MIBG uptake is reduced in patients with heart failure. A new method was developed to reconstruct the semiautomated cardiac ROI in a sympathetic denervated heart. MATERIALS AND METHODS Using dynamic planar data, a summed image was generated and the matrix size was changed. Then, the radial count profiles originating from the center of the left ventricle were generated to extract the myocardial count profiles. An asymmetric Gaussian distribution was fitted to each profile and the epicardial border was defined by the threshold method. This program was tested in 50 patients, and its reproducibility was validated when compared with the manual tracing method. RESULTS The semiautomated method yielded a better quality image compared with the standard image with higher counts. Cardiac ROIs were generated successfully in each patient within normal limits. The intraobserver and interobserver agreements were excellent (P<0.0001 each). This approach showed a significantly higher consistency in measuring the heart-to-mediastinum ratio as compared with the manual tracing method (P<0.05). CONCLUSION The semiautomated method is useful in generating cardiac ROIs with high reproducibility in myocardial MIBG imaging.

Extracting and Mathematical Identifying Form of Stationary Noise in X-ray Images

Abstract: Image noise may prevent proper diagnostic X-ray imaging. This study is aimed at developing new noise rejection methods using a mathematical model that describes the form of X-ray image noise. Stationary noise is one type of noise found in X-ray images. Stationary noise is nonstochastic and appears independent of the radiographic factors. In this paper, we verify methods for identifying stationary noise using a polynomial regression model, and extracting such noise from X-ray images obtained from a CR system. The results of this study demonstrate that stationary noise can be extracted with high precision using a particular low-pass filter frequency. We found that a regression model for greater than second-degree polynomials can be applied for roughly identifying stationary noise. However, the fitting accuracy of the regression curve is not significantly improved in terms of the amount of multiplication required when increasing the degree of the polynomial regression model. Ke yw ords: X-ray Image, Nonstochastic Noise, Stationary Noise, Polynomial Regression Model and CR System