Nagoya Women's University

About: Nagoya Women's University is a education organization based out in Nagoya, Japan. It is known for research contribution in the topics: Soleus muscle & Skeletal muscle. The organization has 190 authors who have published 307 publications receiving 3836 citations.

Sesamolin Inhibits Lipid Peroxidation in Rat Liver and Kidney

Abstract: Although the sesame lignans, sesaminol and sesamolinol, have been shown to possess antioxidative activity, less is known about the metabolism and antioxidative properties of sesamolin, a major constituent of sesame oil. To determine the ability of sesamolin to act as an antioxidant in vivo, we fed rats a diet containing 1% sesamolin for 2 wk and studied its metabolism and its effects on oxidative stress. About 75% of the ingested sesamolin was excreted unmetabolized in feces, but it was not detected in urine. Sesamolin and its metabolites, sesamol and sesamolinol, were excreted primarily as sulfates and glucuronides. The amount of sesamolin and its metabolites was lower in the plasma than in the liver or kidneys. When we compared rats fed a diet containing 1% sesamolin for 14 d with those fed a control diet, we found that liver weight was significantly greater in the former group. Lipid peroxidation activity, measured as 2-thiobarbituric acid reactive substances, was significantly lower in the kidneys and liver of the sesamolin-fed rats than in the controls. In addition, the amount of 8-hydroxy-2'-deoxyguanosine excreted in the urine was significantly lower in the sesamolin-fed rats. These results suggest that sesamolin and its metabolites may contribute to the antioxidative properties of sesame seeds and oil and support our hypothesis that sesame lignans reduce susceptibility to oxidative stress.

Willingness to communicate: can online chat help?1

Abstract: This study compared the experiences of small groups of female Japanese university students communicating in English to solve tasks using online chat with those who solved the same tasks in face-to-face settings using spoken language. The groups were compared using a counterbalanced research design so that the nine groups consisting of four participants each took part in solving tasks in both the online mode and the traditional face-to-face setting. Data gathered from questionnaires, along with an analysis of the discourse produced by students, led to the conclusion that under the conditions in the study, online chatting provided a more comfortable environment, enhancing students’ willingness to communicate. Regarding benefits to the language teacher, online chat provides another fruitful tool to enhance interaction in the target language.

Purification and Properties of L-Galactono-γ-lactone Dehydrogenase, a Key Enzyme for Ascorbic Acid Biosynthesis, from Sweet Potato Roots.

Abstract: L-Galactono-gamma-lactone dehydrogenase (L-galactono-gamma-lactone:ferricytochrome c oxidoreductase [EC 1.3.2.3], GLDHase) which catalyzes the terminal step in the biosynthesis of L-ascorbic acid (AsA) has been purified from roots of sweet potato (Ipomoea batatas L., cv. Kintoki). Highly purified preparation of the GLDHase was obtained by three column chromatography steps with a recovery of ca. 1%, after solubilization from mitochondria in sweet potato roots. SDS-PAGE exhibited a single band at 56 kDa. In the native state, the apparent molecular mass of the enzyme was 56 kDa, based on a Sephadex G-100 gel filtration. The pI and optimum pH values were 5.8 and 7.9, respectively. The Km value for L-galactono-gamma-lactone was 0.12 mM. Substrate inhibition was obtained at concentrations greater than 4.2 mM. The enzyme was inhibited by p-chloromercuribenzoate (PCMB) and acriflavine, and the inhibition of acriflavine was diminished by the addition of FAD or FMN. The only effective substrate for the GLDHase was L-galactono-gamma-lactone.