North China Coal Medical University

About: North China Coal Medical University is a based out in . It is known for research contribution in the topics: Genotype & Apoptosis. The organization has 555 authors who have published 354 publications receiving 6159 citations.

[Changes of serum matrix metalloproteinases-9 and tissue inhibitors of metalloproteinases-9 in coal workers' pneumoconiosis].

Abstract: Objective To explore the changes and significance of matrix metaslloproteinases (MMP) -9 and tissue inhibitors of metaslloproteinases(TIMP)-9 in serum of patients with coal workers' pneumoconiosis(CWP). Methods Serum MMP-9 and TIMP-9 were determined by sandwich ELISA in 188 cases of coal workers' pneumoconiosis (including 53 cases of coal pneumoconiosis, 67 cases of coal-silica pneumoconiosis and 68 cases of silicosis), 57 cases of 0~+ miner (group 0~+), 64 cases of healthy miner (dust exposed control) and 50 healthy people (healthy control), and were further evaluated according to stage, complication, and the duration of exposure to dust in CWP group. Results The median of serum MMP-9 in coal pneumoconiosis, coal-silica pneumoconiosis and silicosis were 17.16, 15.14 and 17.50 ng/ml respectively. The levels of serum MMP-9 in silicosis and coal-silica pneumoconiosis were lower than that in dust exposed control and 0~+ group (P 0.05). There was a positive correlation between age and the concentration of T1MP-9 in silicosis (r=0.249, P<0.05). A positive correlation was shown between the levels of serum MMP-9 and TIMP-9 only in dust exposed control (r=0.294.P<0.05). Conclusions The levels of serum MMP-9 and TIMP-9 are associated with the harmful effect to miner resulted from dust. The apparent disturbance of MMP-9 and TIMP-9 might take part in the pathogenesis of CWP. Dynamic observation of its concentration in group 0~+ and health miners might contribute to the prevention, early diagnosis and differential diagnosis of CWP. Key words: Silicosis; Matrix metalloproteinases; Tissue inhibitors of metaslloproteinases

Relativity between EBV infection and expression of Cyclin D1 in gastric carcinoma patients

Abstract: AIM:To investigate the relativity between the Epstein- Barr Virus (EBV) infection and the expression of Cyclin D1 in gastric carcinoma (GC) patients. METHODS: EBER_1 in 217 cases of GC was detected with in situ hybridization. Then, EBV positive and negative GC cases were selected for analysis of the expression of Cyclin D1 by immunohistochemistry. RESULTS: In 217 GC cases there were 23 cases with EBER_1 positive expression. The expression of Cyclin D1 was higher in the EBV positive GC than that in the EBV negative (P0.01). CONCLUSION: EBV infection in GC may upregulate the expression of Cyclin D1.

Application of the BP Nerve Network Model in the Analysis of Factors Affecting Hospitalization Expenses

Abstract: Collected 2218 medical records of cerebral infarction patients from 2007 to 2008 in a third-class hospital in Tang Shan as sample. A statistical analysis was made of the hospitalization expenses and its influencing factors by BP nerve network model to identify the factors affecting hospitalization expense by using BP nerve network model. The results showed that the major factors impacting the hospitalization expense included age, hospitalization day, therapeutic result and emergency medical treatment. The sensitivity were: age is 0.91426, hospitalization day is 0.32131, therapeutic result is 0.19722, emergency medical treatment is 0.27564. BP nerve network model is a powerful analytical tool for hospitalization expenses and its influencing factors analysis.

Effect of a Single Period of Mechanical Strain on Gene Expression Patterns of Ets1 and Cbfa1 in Murine Calvarial Sutural Osteoblast-Like Cells

Abstract: Cranial sutures produce new bone at the sutural edges of the bone fronts in response to external stimuli. Little is known regarding the mechanism of osteogenesis in cranial sutures. Ets1 and Cbfa1 are two important osteogenic transcription factors regulating the differentiation and maturation of osteoblasts. But their function in cranial sutures is not still elucidated. We have investigated the gene expression of Ets1 and Cbfa1 in rat’s calvarial sutural osteoblast-like cells under a single period of mechanical strain. The cells were isolated from the cranial suture of SD rats and cultured in vitro, and subjected to a single 40 minutes mechanical strain using a four-point bending apparatus. The gene expression patterns of Ets1 and Cbfa1 were examined by RT-PCR. Both mRNA levels of Ets1 and Cbfa1 have increased significantly within 6 and 12 hours respectively after mechanical strain were applied, and the increase returned to control level thereafter. However, Ets1 and Cbfa1 exhibited different temporal expression patterns: Ets1 expressed immediately after the mechanical loading and reached the maximum transcription at 0.5h; whereas Cbfa1 experienced a latency period first, then increased slowly within 2 hours, and reached the maximum transcription at 6 h. The maximum transcription of Cbfa1 was about 2.58 fold of that of Ets1. Ets1and Cbfa1 may play different roles in regulating bone matrix protein expressions in osteoblast-like cells during suture distraction and their function is time-dependent. High frequency distraction (>2times/24h) is favourable to the maximal expression of the two genes.

A posteriori error estimation of the finite element solutions for elliptic double obstacle problems

Abstract: A posteriori estimation of the finite element solutions for double obstacle problems is obtained. There has been a well-established theory and a large number of a posteriori error estimations for linear elliptic one-sided obstacle problems. We will show that the estimation for ∥ z - u h ∥1 is also suited for double obstacle problems.