Showing papers in "Archives of Biochemistry and Biophysics in 1964"

TL;DR: A comparison has been made between streptavidin and avidin from egg white using microbiological, physical, and chemical methods as mentioned in this paper, showing the absence of hexoses and amino sugars associated with avidin and reveals differences in amino acid content.

TL;DR: A method has been developed for quantitatively estimating the 4 cytochromes a, b, c 1 , and c simultaneously in a single mitochondrial sample using a simplified procedure for the solution of 4 simultaneous equations in 4 unknowns.

TL;DR: In this paper, an unbuffered solution of isolated spinach chloroplasts manifests an increase in pH upon illumination which is reversed by turning the light off, and the rate of the pH change and the amount of acid required to prevent this change (yield) are strongly stimulated by various cofactors which support photosynthetic phosphorylation.

TL;DR: The present kinetic analysis quantitatively reconfirmed the previous conclusion that o-phenanthroline and adenosine diphosphate ribose interact independently with different sites of the enzyme.

TL;DR: The transport systems of Salmonella typhimurium for histidine and for the aromatic amino acids have been studied and a new parameter is introduced: the limit concentration, which represents the minimal external concentration of an amino acid at which the organism utilizes exclusively the external supply and completely stops the biosynthetic production of that amino acid.

TL;DR: Two new reactions in the final stages of blood coagulation that involve the fibrin stabilizing factor of plasma are described, where the factor is first activated by thrombin in the presence of calcium ions.

TL;DR: Gamma globulin in high yield has been separated from undialyzed normal human serum by a simple, economical, and rapid two-stage batch procedure by employing the chloride form of DEAE-Sephadex at pH 6.5 as mentioned in this paper.

TL;DR: The conversion of IAN to IAA and ammonia is quantitative, requires no oxygen, and does not yield the free amide as an intermediate product, so that the two stages of hydrolysis are probably carried out by one and the same enzyme.

TL;DR: Equations relating the elution volume in Sephadex gel filtration to the molecular weight and the molecular radius of a given solute have been derived on the basis of a model in which the elements of volume available to solvent within the gel are approximated by a combination of cones, cylinders, and crevices.

TL;DR: Heart mitochondria accumulate large amounts of Ca ++ and inorganic phosphate and release H + into the medium when incubated under appropriate conditions, but accumulation is inhibited by oligomycin and by azide, and also by aging of the mitochondria.

TL;DR: The sulfhydryl groups in chicken ovalbumin, bovine β-lactoglobulin, and Bovine serum albumin were studied with four different types of chemical reagents: p-chloromercuribenzoate, iodine, N-ethylmaleimide and 5,5′-dithiobis (2-nitrobenzoic acid) as mentioned in this paper.

TL;DR: It is suggested that the primary action of the non-mutant ( Bm 1 ) gene at the bm 1 locus is to exert a measure of control over the manner of incorporation of the phenolic building blocks into the lignin polymer.

TL;DR: Rabbit cardiac myosin contains fewer cysteine residues than the skeletalMyosin and the ATPase activities of both cardiac and skeletal myosins, actomyosin, and myofibrils, however, are close to each other when determined in the presence of Mg ++ at high ionic strength.

TL;DR: A number of dyes that are active in other photodynamic reactions also sensitize the reaction of guanine derivatives and have little or no effect on the other bases which occur in DNA and RNA.

TL;DR: Structural protein prepared in this manner combines spontaneously with micellar phospholipids at neutral pH and the interaction is qualitatively and quantitatively similar to the rebinding of phospholIPids by “lipid-deficient” mitochondria.

TL;DR: The mechanism of sucrose-starch transformation was studied in corn endosperm and the following two sequences for the glucose incorporation into the granule were proposed: SucroseαADP-glucose (or UDP- glucose)→starch (b) Sucrose αUDP-glUCoseαglucOSE-1-PαADp-glugose→st starch.

TL;DR: The yeast Candida utilis assimilates 5′-methylthioadenosine from the culture medium and converts it into intracellular S -adenosylmethionine, and specific activity recovered was lower when methyl-C 14 -labeled material was used.

TL;DR: Mitochondria partially swollen by l -ascorbate can be contracted with ATP and Mg ++ only if liberated fatty acids are removed by serum albumin and further peroxidation is blocked with EDTA.

TL;DR: The high molar activity of carbonic anhydrase, which was not significantly changed in the fresh water animals, indicates that this enzyme can only play a secondary nonlimiting role in the salt secretion process.

TL;DR: The “permissible” effect of glucocorticoids on fat mobilization is attributed, on the basis of the obtained results, to their modifying influence on carbohydrate metabolism of adipose tissue.

TL;DR: The results show that the inhibition of phosphorylase by UDPG is not a prerequisite for glycogen accumulation, although inhibition by other NDPG compounds such as TDPG and ADPG may play a role in vivo.

TL;DR: It was shown that the esterification activity of plasma from a variety of sources could be evaluated with this common substrate and that cholesterol esters of individual fatty acids were formed in quantities proportional to the pre-existing pattern of the active plasma added.

TL;DR: The possibility that the lysosomal esterase is involved in the control of the permeability of the lYSosomal membrane, rather than intralysosome autolysis of subcellular particles, is discussed.

TL;DR: It is shown that tyrosine- O -phosphate is a normal and prominent constituent of third instar larvae of Drosophila and accumulates during the rapid growth period following the second molt and disappears abruptly at puparium formation.

TL;DR: The moderate biological activity of 2,4-dichlorophenoxyacetonitrile in pea stems, which do not contain nitrilase, is shown, both from biochemical considerations and from comparative bioassay, to be probably due to relatively slow nonenzymatic hydrolysis.

TL;DR: In vivo, the seryl derivative causes in vivo no or only weak inhibition of other enzymes, such as monoamine oxidase, diamine oxid enzyme, catecholamine-O-methyl transferase, transaminase of aromatic amino acids, tryptophan hydroxylase; in vitro, the Seryl derivative does not affect the histidine decarboxylase or dopamine-β-oxidase.

TL;DR: The catalytic and end-product inhibition characteristics of this enzyme from various species were compared and the possible meaning of the differences in the characteristics of ATCase from the various organisms was discussed.

TL;DR: Results indicate that some of the tyrosyl residues of native ribonuclease and insulin are so situated that their fluorescence is abolished or severely diminished and that those residues are transformed into a more normal environment during denaturation.

TL;DR: It has been confirmed that urea increases the measured pH of aqueous solutions and conductance measurements on acetic acid and colorimetric measurements on p -nitrophenol show that u Andrea increases the dissociation of weak acids.