Archivum histologicum Japonicum 

About: Archivum histologicum Japonicum is an academic journal. The journal publishes majorly in the area(s): Endoplasmic reticulum & Golgi apparatus. It has an ISSN identifier of 0004-0681. It is also open access. Over the lifetime, 309 publications have been published receiving 6493 citations.

Application of the scanning electron microscope to the study of the fine distribution of the blood vessels.

Abstract: The fine distributions of the blood vessels can be readily and clearly demonstrated by preparing their solid corrosion casts and by observing the corrosions thus obtained under the scanning electron microscope. As examples, the scanning electron micrographs of the capillary meshes of the rat's renal glomerulus, gastric mucosa, intestinal villi and hepatic lobules reproduced with methacrylic methyl ester resin were shown. And the procedure employed in the casting and scanning electron microscopy was described. In the casting methods, some improvements were made to reproduce delicate capillaries such as those of the villi of the small intestine.

A Revised Tannin-Osmium Method for Non-Coated Scanning Electron Microscope Specimens

Abstract: A revision in preparation technique for non-coated scanning electron microscope specimens is reported. Use of arginine hydrochloride, glycine, sodium glutamate and sucrose before treatment with tannic acid markedly enhanced the osmium staining of biological specimens and enabled direct scanning electron microscope observation at magnification as high as 30, 000×. The advantages of this method were discussed.

Functional classification of cell types of the anterior pituitary gland accomplished by electron microscopy.

Abstract: Recent progress in endocrine physiology showed six different hormones secreted from the anterior pituitary gland, but their cellular sources were not completely elucidated, when the morphological technique was confined to the light microscopy. Application of the electron microscope is very advantageous to the field of functional morphology of the pituitary gland. Within a few years, the identification of cellular sources of the six known hormones of the anterior pituitary has been established in the rat and mouse.Methods for determination of endocrine function of each cell type distinguished by electron microscopy are discussed in detail. Some of them have been fully utilized by electron microscopists who are engaged in functional morphology of the anterior pituitary gland, but a few remaining ones are still in the trial stage.The nomenclature of the anterior pituitary cells indicating the morphological characteristics is now replaced by a new terms implying the function, namely indicating the hormone which is secreted by a given cell type.As the rats have been most frequently observed and best known as to function, the description of this paper is concentrated to the rat anterior pituitary. The electron microscopical features of each of the seven cell types in either normal, pathological or experimental condition are described, and some representative electron micrographs are illustrated.

Electron microscopic study on the hepatic sinusoidal wall and the fat-storing cells in the normal human liver.

Abstract: In normal human livers obtained in surgical operations the sinusoidal wall has been observed with the electron microscope.The “endothelial cells” and the “Kupffer cells” lining the sinusoid are regarded as two functional states of the same cell lineage and show alike the ultrastructures suggesting phagocytic activity. They differ from each other especially in that the endothelial cells consist of two distinct portions, the perikaryon and the sheet-like cytoplasmic extension, the latter occupying the major part of the sinusoidal lining, while the Kupffer cells are of simple rounded-up form bulking into the sinusoidal lumen; the endothelial cell sheets extend to the Kupffer cell body to contact with the latter. On the other hand, the marginal ends on the neighboring endothelial sheets may overlap like roof tiles. In both connections there occur no functional structures like terminal bars and desmosomes.The endothelial lining of the sinusoid which is discontinuous because of the presence of intracellular gaps or pores and lacking in general in continuous basement membrane, is as a rule a simple layer. Frequent figures of stratified endothelial sheets are caused for the most part by the occurrence of subendothelial fat-storing cells which extend thin processes as if to reinforce the endothelial lining. The perisinusoidal or Disse's space is filled with blood plasm filtered through the discontinuous endothelial lining of the sinusoid. This space which represents the blood-tissue barrier of the liver contains, besides abundant microvilli of the hepatocytes and collagen or reticular fibers and fibrils, fat-storing cells, unmyelinated nerve fibers with their Schwann cells and occasional round, probably hemopoietic cells. The fat-storing cells are constant residents in the Disse's space and frequently extend into the recesses between the hepatocytes. They are lacking in their own basement membrane and contact directly with the collagen fibers, which are sometimes embraced in the invaginations of their plasma membrane. They send out a considerable number of cytoplasmic processes in the Disse's space which interlace with the microvilli of the hepatocytes. Close contacts between the even surfaces of the fat-storing cells and of the hepatocytes are occasionally encountered.The fat-storing cells possess relatively well-developed Golgi complex containing a diplosome, sparse small mitochondria, fairly well-developed elements of granular endoplasmic reticulum, small lysosomes, glycogen particles and, occasionally, a single cilium into the Disse's space which arises from one of the paired centrioles of the diplosome. Though the fat-storing cells exhibit no cytological signs indicating a phagocytic activity, many invaginations of the plasma membrane and vesicles including the bristle-coated ones are found along their free surfaces suggesting a vigorous pinocytotic activity. As constant and characteristic cytoplasmic inclusion bodies, the fat-storing cells contain small fat droplets, each of which (about 2μ in diameter) is bounded by a weak limiting membrane and does not fuse with each other into larger ones. The fat-storing cells may occasionally contain no fat droplets; the “pericytes” observed by some authors in the Disse's space, probably correspond to these empty fat-storing cells.The following possible functions of the fat-storing cells are proposed: 1) Fat synthesis and fat-droplet formation, 2) the storing of fat droplet and fat-soluble vitamin A, 3) the participation in intralobular fibrilogenesis and 4) the reinforcement of the endothelial lining of the sinusoid by sending subendothelial processes.

Microcirculation of monkey pancreas with special reference to the insulo-acinar portal system. A scanning electron microscope study of vascular casts.

Abstract: Methacrylate casts of the pancreatic blood vessels of the monkey were observed under the scanning electron microscope. Microdissection and scanning of the casts were repeated in order to examine the deeper parts of interest successively.It was demonstrated that a vas afferens, a branch of the intralobular artery, enters deep into the islet which is represented by a three-dimensional net of irregularly swollen capillaries. The termination of the vas afferens at the center of the islet in the monkey was discussed in relation to the central position of A cells in this animal.From the periphery of the islet numerous vasa efferentia radiate into the exocrine tissue to be connected, at a certain distance, with the capillary network of the latter. They are refered to as insulo-acinar portal vessels as they convey the islet hormones to their first target tissue, the exocrine pancreas.The capillaries in the exocrine part are gathered into numerous venules whose confluent forms the intralobular vein.The vas afferens of the islet only occasionally issues a branch directly supplying the exocrine tissue. Otherwise, the latter tissue receives all its blood through the capillaries of the islet.