Showing papers in "Biologia Plantarum in 1970"
TL;DR: Stomatal frequency in leaves of plants of Iris hollandica, cultivated under four light intensities, about 12%, 37%, 75% and 100% of natural daylight, was studied.
Abstract: Stomatal frequency in leaves of plants of Iris hollandica, cultivated under four light intensities, about 12%, 37%, 75% and 100% of natural daylight, was studied. The stomatal frequency decreases with lower light intensities. The gradients of stomatal frequency of successive leaves show an increase up to the third or fourth leaf with a subsequent decrease of values up to the highest leaf. The total number of stomata per leaf is not constant; differences exist in successive leaves of a shoot as well as among the leaves of plants growing under various light intensities. The character of gradients was changed at lower light intensities. The largest reaction to the light intensity appeared in leaves with higher stomatal frequency. The gradients in individual leaves depend on the insertion of the leaf. Both the shape and the slope of these gradients are influenced by the light intensity.
27 citations
TL;DR: In this paper, the effect of phosphorus deficiency on anthocyanin content in tomato plants was studied from the point of view of pathological growth inhibition, and the results of the experimental variant with enhanced phosphorus concentration in nutrient solution were not uniform.
Abstract: The effect of phosphorus deficiency on anthocyanin content in tomato plants was studied from the point of view of pathological anthocyanin formation. Phosphorus deficiency resulted in an evident increase of anthocyanin content in all experiments, independently of the tomato variety and of the cultivation conditions. The anthocyanin amount was five times higher on the average and the differences were statistically significant. The average growth inhibition of tomato plants under phosphorus deficiency was 18%. Accumulation of anthocyanins is limited by the temperature; anthocyanins are not synthesized at temperatures above 30°. The results of the experimental variant with enhanced phosphorus concentration in nutrient solution were not uniform. In some experiments a decrease of anthocyanins accompanied by slight growth stimulation was observed, in others an increase associated with growth inhibition. It seems that cultivation conditions, especially the light intensity, play an important role in these experimental variants.
25 citations
TL;DR: D-Try is metabolised in the three plants by the virtually exclusive formation of malonyltryptophan, which could be demonstrated only by bioassay.
Abstract: The metabolism of D-and L-tryptophan-3-14C (Try-3-14C) was studied and compared for three different plant species, cabbage, maize and pea Apical segments of the seedlings were incubated for 6 hours in solutions of L- or D-Try-3-14C (1·5 μc/ml) with the addition of chloramphenicol (10−4g/ml) and then allowed to stand for another 20 hours in moist chambers The methanolic extract of the tissues was analyzed radiochromatographically and by paper electrophoresis in combination with biological tests Chloramphenicol in a concentration of 10−4 g/ml had little influence on the growth of the segments, though the antibiotic slightly decreased the uptake of L-Try, it did not prevent the formation of IAA from L-Try In the segments of cabbage the following metabolites were formed from L-Try-3-14C (accounting for 52% of the activity of the chromatographically separated extract): glucobrassicin (26·0%), neoglucobrassicin (3·6%), a spot corresponding according to its Rf to 3-indolylacetamide (IAAmide—10·9%), β-glucoside of 3-indolylacetic acid (IAGluc—3·3%) and traces of 3-indolylacetonitrile (IAN), IAA and indole-3-carboxylic acid (total 5%) In maize segments L-Try-3-14C (53·0%) was transformed to several unidentified hydrophilic substances, one of them possessing auxin activity (total amount 6·9%), IAGlue (9·3%) accompanied by a small amount of tryptamine, a spot corresponding according to its Rf to IAAmide (16·5%), IAA and another unidentified hydrophobic substance (4·1%) In pea segments L-Try-3-14C (66·7%) gave a zone corresponding according to its Rf to IAAmide (20·0%), a substance similar to IAGluc (10·5%) and also hydrophobic substances (3·1%) containing traces of IAA, which could be demonstrated only by bioassay D-Try is metabolised in the three plants by the virtually exclusive formation of malonyltryptophan
22 citations
TL;DR: It is suggested that synthesis of some specific protein is required for senescence in the detached leaf tissue of etiolated seedlings of maize and this protein was possibly arrested by the compounds which were under examination.
Abstract: Kinetin, coumarin and four growth retardants including Phosfon D (2,4-dichlorobenzyltributylphosphonium chloride), CCC [(2-chloroethyl) trimethylammonium chloride], B-Nine (N-dimethylaminosuccinamic acid) and AMO-1618 (2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidinecarboxylate methyl chloride) arrested chlorophyll, protein and RNA degradation in the leaf discs of maize kept in darkness; GA3 was without effect. Coumarin and Phosfon D markedly lowered the level of TCA soluble nitrogen compounds in the tissue; other compounds were inactive in this respect. Puromycin, 4-fluorophenylalanine, actinomycin D, 5-diazouracil and 2-thiouracil also retarded the loss of chlorophyll from the leaf discs of maize; chloramphenicol was without effect on yellowing. The inhibitors of RNA and protein synthesis slightly decreased, increased or had no influence on the chlorophyll preserving effect of coumarin and growth retarding chemicals. 2-Thiouracil markedly decreased the loss of protein content from the discs.
17 citations
TL;DR: In this paper, the authors compared conductivity in 5 mm long segments from the basis of the primary seminal root with that in segments from adventitious roots of the first to fifth node and found that the conductivity is not a general function of the transection area.
Abstract: When comparing conductivity in 5 mm long segments from the basis of the primary seminal root with that in segments from adventitious roots of the first to fifth node it was revealed that the conductivity is not a general function of the transection area. The conductivity determined experimentally is minimal in the primary seminal root and gradually increases in the adventitious roots. However, when calculating per area unit, roots of the first coleoptile node appear the most efficient, with roots of the second and third node the values gradually decrease, and conductivity in roots of the fourth and fifth node approximately equals that of the primary seminal roots.
13 citations
TL;DR: The tapetal layer of anthers inCapsicum annuum L. annuum is differentiated from the archesporial complex during the early development stage of theAnthers and further development of tapetum proceeds according to the scheme of the cellular polynuclear type.
Abstract: The tapetal layer of anthers inCapsicum annuum L. is differentiated from the archesporial complex during the early development stage of the anthers. Further development of tapetum proceeds according to the scheme of the cellular polynuclear type. The high rate of polyploidy is characteristic of the whole layer (from 4n to 8n). Cytokinesis does not follow karyokinesis which conditions the inception of two or three-nuclear cells. The majority of the mononuclear cells show the typical plant endomitosis without the chromosome spiralization, accompanied by the structural nuclear change-over. In other cells chromosome spiralization in the prophase and, after passing through the metaphase, restitution of the cell nuclei were observed. The tapetum development has been studied in 12 cultivars of sweet pepper which did not differ in the course of the karyological processes. The subtle differences during the temporal course of degeneration of the whole layer in the postmeiotic period of development were observed.
9 citations
TL;DR: There was a significant decrease in concentration of SBMV in juice extracted from anthocyanized beet plants as compared with extracts from normal green infected plants, and the intensity of TMV symptoms in beet leaves was considerably decreased in leaves of antho-eyanized plants.
Abstract: During our studies on the interaction of anthocyanins and plant virus diseases, reproduction of sugar beet mosaic (SBMV) and tobacco mosaic viruses (TMV) was investigated. Experiments were carried out in leaves of sugar beet,Beta vulgaris cv. Dobrovicka N and its spontaneous anthocyanized mutant. SBMV induces a systemic infection while TMV is responsible for primary local symptoms in sugar beet leaves only. Our quantitative analyses onAmaranthus caudatus L. andChenopodium quinoaWilid. showed a significant decrease in concentration of SBMV in juice extracted from anthocyanized beet plants as compared with extracts from normal green infected plants. Significant differences were also obtained when SBMV — containing juice was tested in mixtures with healthy extracts from anthocyanized and normal green plants. Also the intensity of TMV symptoms in beet leaves was considerably decreased in leaves of antho-eyanized plants.
8 citations
TL;DR: The results suggest that the concentration of PMA normally applied as an antitranspirant is unfavourable for healthy growth of maize and barley.
Abstract: A reduction of stomatal aperture in light was found in leaves of maize after they had been treated with 10“3-5 m phenylmercuric acetate (PMA) Complete closure of the stomata in darkness was prevented, whilst there was total closure in the controls Higher PMA concentrations had bigger effects The relative water content (RWC) of barley tissues was slightly reduced 12 hours after treatment with PMA The transpiration rate observed on PMA-treated barley plants was lower in light and higher in darkness than in untreated plants Water saturation deficit (WSD) was higher by about 5%, and water holding capacity (WHC) lower (25%) than in untreated plants The results suggest that the concentration of PMA normally applied as an antitranspirant is unfavourable for healthy growth of maize and barley
8 citations
TL;DR: Embryogenesis occurred in carrot root callus cultivated on simple synthetic medium containing IAA and 2,4-D and confirmed reports about the organogenic potentiality of this species and about its sensitivity to some chemical substances.
Abstract: Embryogenesis occurred in carrot root callus (Daucus carota L.) cultivated on simple synthetic medium containing IAA and 2,4-D. Embryoid development continued also during successive years when the tissue was cultivated on the same nutrient medium without those substances. Sometimes production of plants with atypical leaves was also observed. In those plants development of adventive embryoids occurred repeatedly. Result of this work confirmed reports about the organogenic potentiality of this species and about its sensitivity to some chemical substances.
8 citations
TL;DR: In the roots of Dendrobium cunninghamii, a state of balanced symbiosis exists between the fungus and the plant; a part of the time the plant acts as a parasite on the fungus.
Abstract: The root cap develops out of the cells of the caliptrodermatogen. The root apex belongs to the first of Haberlandt’s histogen types. The velamen usually consists of three layers of cells, and develops out of the protoderm by periclinal divisions. Funnel-shaped cells later divide tangentially into two cells, and in one of these cells a fibrous body develops. Two to four thick-walled cells alternate, usually with one passage cell in the exodermis. The cortex proper consists of approximately ten to twelve layers of parenchymatic cells in the mature root. There are eight to sixteen xylem poles alternating with the same number of phloem poles in the central cylinder.
7 citations
TL;DR: The stimulating effect of light on the germination of non-stratified apple embryos was demonstrated, independent of season and seems to be independent of the effects of growth regulators.
Abstract: Experiments were aimed at checking whether the non-stratified apple embryos are sensitive to growth factors and light. The effect of growth regulators on light sensitivity was also studied. The stimulating or inhibiting effect of GA3, benzyladenine and IAA or coumarin on the dynamics of apple embryo germination was demonstrated. Seasonal fluctuations of the effect of the growth factors were noted. The stimulating effect of light on the germination of non-stratified apple embryos was demonstrated. This effect is independent of season and seems to be independent of the effects of growth regulators.
TL;DR: It follows that in apical buds of Chenopodium rubrum there exists a mutual interaction between gibberellins and cytokinins, and under certain conditions both these groups of hormones may substitute for each other in flowering.
Abstract: CCC (2-chloroethyl)trimethylammonium chloride applied to plants ofChenopodium rubrum during floral induction led to an increase in the level of endogenous cytokinins in the apical buds Application of gibberellic acid or indole-3-acetic acid at concentrations reversing the effect of CCC reduced the level of cytokinins After simultaneous treatment with both CCC and one of the growth substances this reduction was less pronounced From the comparison bf the present results, as well as of those published in previous papers it follows that in apical buds ofChenopodium rubrum there exists a mutual interaction between gibberellins and cytokinins Under certain conditions both these groups of hormones may substitute for each other in flowering IAA seems to affect flowering by regulating the level of both gibberellins and cytokinins
TL;DR: It can be assumed that 6-B and its metabolites are transported in one-year old apple shoots acropetally through the xylem and basipetallythrough the phloem, while penetrating from the Xylem to the phLoem.
Abstract: The transport of radioactivity of 6-benzyladenine-8-14C applied to one-year old apple shoots was studied. Simultaneously, labelled metabolites of this cytokinin were studied separately in the xylem and phloem above and below the place of application. According to the results obtained, it can be assumed that 6-B and its metabolites are transported in one-year old apple shoots acropetally through the xylem and basipetally through the phloem, while penetrating from the xylem to the phloem. Besides 6-B-8-14C, a complex of cytokinin with sugar, also adenosine and adenine were found in the phloem.
TL;DR: The minimum concentration of polyethyleneglycol (PEG) with molecular weights 4000, 6000, and 15000 necessary for precipitation of S, M, X and Y potato viruses was determined in this article.
Abstract: The minimum concentration of polyethyleneglycol (PEG) with molecular weights 4000, 6000, and 15000 necessary for precipitation of S, M, X and Y potato viruses was determined. An excessive amount of PEG causes the precipitation of other protein compounds from potato leaf cell sap. In order to obtain highly purified samples, it is necessary to use just the minimum sufficient amount of PEG. Using the minimum quantity of PEG is, also, advisable from an economical point of view. The minimum concentration of PEG of given molecular weight differs for different potato disease viruses. The concentration of PEG necessary for precipitation of a given potato virus depends on the molecular weight of PEG used—4000, 6000 and 15000. As the molecular weight increases, the concentration of PEG necessary for precipitation decreases.
TL;DR: Metabolites of indolepyruvic acid and indolelactic acid were investigated using 2 systems: a bacterial (pea stem homogenates containing the epiphytic bacteria) and a plant system ( pea stem sections under sterile conditions).
Abstract: Metabolites of indolepyruvic acid and indolelactic acid were investigated using 2 systems: a bacterial (pea stem homogenates containing the epiphytic bacteria) and a plant system (pea stem sections under sterile conditions). The products of spontaneous indolepyruvic acid decomposition in aqueous solution and during chromatography were investigated, too.
TL;DR: In this paper, a positive linear correlation between the resistance to water transport in liquid phase and water saturation deficit (w.s.d.) in the tissue of Brassica oleracea andNicotiana tabacum leaves was observed.
Abstract: Positive linear correlation between the resistance to water transport in liquid phase and water saturation deficit (w.s.d.) in the tissue ofBrassica oleracea andNicotiana tabacum leaves was observed. At the same values of w.s.d. corresponding values of the resistance to water transport were higher when dehydration of the leaf tissue occurred during the experiment and lower when water balance was in equilibrium or resaturation of the leaf tissue occurred.
TL;DR: In this paper, the effect of simple phenolic glycosides on the activity of IAA-oxidase isolated from gherkin seedlings was studied in experimentsin vitro. But the effect was limited to the case when β-glycosidase was present: the phenols released from their bound form increased or decreased the IAA level.
Abstract: The effect of phenols and simple phenolic glycosides on the activity of IAA-oxidase isolated from gherkin seedlings was studied in experimentsin vitro. Phenol stimulated the enzyme system activity, eugenol and quinol were proven as inhibitors. Simple phenolic glycosides (arbutin, gein and phenol glucoside) influenced IAA-oxidase activity only if β-glycosidase was present: rree phenols released from their bound form increased or decreased the IAA level. The potential fegulatory effect of simple phenolic glycosides on the IAA level in plants has been discussed; this effect is thought to be mediated by free phenols and by influence on the IAA-oxidase system.
TL;DR: Antibodies isolated from antiserum against plant viruses were labeled with the isotope35S as follows: the mixture of antibodies with radioactive cysteine hydrochloride was allowed to stand for half an hour, run on a Sephadex G-25 column and individual fractions were collected.
Abstract: Antibodies isolated from antiserum against plant viruses were labeled with the isotope35S as follows: the mixture of antibodies with radioactive cysteine hydrochloride was allowed to stand for half an hour, run on a Sephadex G-25 column and individual fractions were collected. Sephadex G-50 bed was equilibrated and washed with saline (0,85 % NaCl) phosphate buffer (0,01 m) pH 7,2. Fractions showing the highest radioactivity and at the same time the most evident serological reaction were combined and used as35S labeled antibodies. The labeled antibodies were used for rubbing leaves; the leaves were afterwards incubated, washed, killed, dried and then subjected to autoradiography. The method of indirect serological reaction also proved to be very good. Using this method, pig gamma globulin against rabbit gamma globulin was labeled with35 S; this labeled gamma globulin was then used to detect serological reaction on leaves between the virus and homologous rabbit antiserum and/or antibodies. The results of those reactions were also determined by autoradiography.
TL;DR: The testing method for clonal selections from the original strain Scenedesmus quadricaudaGreifswald/15 in biomass production facilitated a more detailed evaluation of clones in comparison with values obtained by an oneshot testing method under standard conditions.
Abstract: The author describes the testing method for clonal selections from the original strainScenedesmus quadricaudaGreifswald/15 in biomass production This method served for testing the clones in greater series It was applied to clonal cultures selected on a solid medium after treatment of the coenobia with UV-light before their inoculation The production characteristic of the clones was expressed by the temperature dependence of the constant μ from Simmer's growth equation in the last step of the testing process used Some significant differences of this kind were found among the evaluated clonal cultures This way of characterization facilitates a more detailed evaluation of clones in comparison with values obtained by an oneshot testing method under standard conditions
TL;DR: Using electrophoresis in acrylamide gel, fractions of peroxidase, indoleacetic acid oxidase, and o-diphenol oxidase were investigated in extracts from three growth zones of Vicia faba L. roots as discussed by the authors.
Abstract: Using electrophoresis in acrylamide gel, fractions of peroxidase, indoleacetic acid oxidase, and o-diphenol oxidase were investigated in extracts from three growth zones ofVicia faba L. roots. Three peroxidase fractions (zones) moving towards the anode were revealed as well as four peroxidase fractions (zones) migrating towards the cathode. Three peroxidase fractions showed detectable indoleacetic acid oxidase activity. The o-diphenol oxidase activity was revealed in all peroxidase fractions moving towards the anode, in those moving towards the cathode the o-diphenol oxidase activity differred according to the substrate used. One fraction with both peroxidase and o-diphenol oxidase activity occurred only in electrophoreograms of extracts from the maturation zone; in this fraction no indoleacetic acid oxidase activity was demonstrable.
TL;DR: The dynamics of the uptake by barley plants of trimer phosphonitrile-amide labelled with32P was studied and it was shown experimentally that the covalent compound enters the plants more rapidly than (NH4)2HPO4.
Abstract: The dynamics of the uptake by barley plants of trimer phosphonitrile-amide [PN(NH2)2]3 labelled with32P was studied. It was shown experimentally that the covalent compound [PN(NH2)2]3 enters the plants more rapidly than (NH4)2HPO4. Hence it was concluded that the mechanisms of the transport of [PN(NH2)2]3 and (NH4)2HPO4 into the cells, across the cell membranes, are different.
TL;DR: The lower the temperature to which the seeds were exposed and the greater the delay in start of germination, the greater were the amounts of amino acids exosmosed from the kernel to the surrounding media.
Abstract: Maize caryopses were exposed in moist, sterile sand to temperatures of 24°, 10° and 6°. The amino acid content of embryo, endosperm and pericarp tissues of kernels and amino acid occurence in sand eluates was analysed by paper chromatography.
TL;DR: The effect of phenolic substances isolated earlier from cabbage, maize and pea on L-tryptophan-3-14C metabolism in those plants was investigated in this paper.
Abstract: The effect of phenolic substances isolated earlier from cabbage, maize and pea on L-tryptophan-3-14C (L-Try-14C) metabolism in those plants was investigated. For the sake of comparison the effect of diethyldithiocarbamate (DIECA) on cabbage was also observed. A phenolic substance of unknown structure isolated from cabbage was utilised in a 0.5 mg/ml concentration, p-coumaric acid (PCA) from maize in 0.7 mg/ml concentration and quercetinglucosyl-coumarate (QGC) found in pea in 8 mg/ml concentration were used. The chosen concentrations were on the limit of their inhibitory effect on the growth of the respective plant apical segments. The effect of DIECA was investigated at 0.1 mg/ml concentration. Using non-labelled L-Try as substrate (5×10−3m) biological tests have shown that QGC and DIECA have a weaker but distinct effect on the increase in auxin level (+17% and +15%, respectively). All phenolic compounds, as well as DIECA increase the intake of L-Try-14C from the incubation solutions. Phenolic substances decrease the conversion of L-Try-14C to its metabolites. The changes were studied after a 20 hour period of L-Try-14C metabolism. On chromatograms changes in the proportion of L-Try-14C metabolites took place, especially in the areas of substances of complex character from which IAA splits off easily,i.e. in the areas of 3-indolylacetylglucose and 3-indolylacetamide. The radioactivity of these areas is generally decreased (e.g. the decrease of radioactivity in the 3-indolylacetylglucose area under the influence of the phenolic substance ofBrassica plants is −7,4%, under the influence of PCA in maize it is −8.9% and under the influence of QGC in pea it is −17.1%). DIECA also decreased the radioactivity of this zone, by −10,5%. In cabbage a large part of L-Try-14C was transformed to glucobrassicin; its biosynthesis increases in the presence of the phenolic substance of cabbage by +3%, in the presence of DIECA by +27%. The results are discussed in a working hypothesis based on the key formation of IAA, accompanied by its oxidation and detoxication.
TL;DR: In this paper, trimeric and tetrameric phosphorus nitridoamide and ammonium diamidophosphate were applied as fertilizers in a vessel experiment with barley and their agrochemical effectiveness was compared with that of ammonium phosphate.
Abstract: Phosphorus-nitrogen compounds characterized by the direct P−N bond, represented by trimeric and tetrameric phosphorus nitridoamide—[PN(NH2)2]3–4, phosphorus oxytriamide—PO(NH2)3 and ammonium diamidophosphate—NH4PO2(NH2)2, were applied as fertilizers in a vessel experiment with barley and their agrochemical effectiveness was compared with that of ammonium phosphate—(NH4)2HPO4. The results obtained indicate that the above compounds are of nutritive value for plants.
TL;DR: In this paper, values of the water saturation deficit (WSD) for hydroactive stomatal movements of kale leaves were estimated using the method of transpiration curve analysis using the transpiration model.
Abstract: Values of the water saturation deficit (WSD) for hydroactive stomatal movements of kale leaves were estimated using the method of transpiration curve analysis
TL;DR: It was established that developing pollen tubes of Oenothera organesis obtain sugar from the stylar tissue through which they grow by the addition of boric acid or calcium to the warm water in which the styles were treated.
Abstract: The addition of boric acid or calcium, or both boric acid and calcium to the warm water in which the styles were treated led to a further enhancement of pollen tube growth in otherwise incompatible styles.
TL;DR: The correlative inhibition of the leaf causes anisophylly in marginal shoots in the same way as that of axillaries inBryophyllum.
Abstract: Growth correlations in leaves ofBryophyllum may be recognized by the development of marginal shoots varying in their particular lamina regions, the correlative inhibition increasing from the top to the base. Cytokinins extend their promoting action inB. crenatum leaves farther in the apical than in the basal direction. In the uppermost leaves ofB. daigremontianum they evoke the development of marginal shoots with flowers in the apical and of vegetative shoots in the basal region. Less suitable for this research are auxins, gibberellins, and triiodobenzoic acid which are unable to stimulate the development of marginal shoots on leaves grown out under short days if not supplemented by cytokinins. Only TIBA induces under long days formation of adventitious shoots directly from the lamina surface on leaves developed below the terminal ring fasciation exhibiting at free ends of connate leaves normal marginal shoots. Under short days a complete reduction of lateral teeth takes place inB. verticillatum both on the extremity of ring fasciation and on the uppermost leaves, the marginal shoots being formed under the subsequent long days only in the angles between the ring fasciation parts. Furthermore the correlative inhibition of the leaf causes anisophylly in marginal shoots in the same way as that of axillaries inBryophyllum.
TL;DR: Activities of five enzymes were followed in electrophoreograms of extracts from three growth zones of broad bean root, and ascorbate oxidase activity occurred in the same regions of electrophorograms as peroxidase activity.
Abstract: Activities of five enzymes were followed in electrophoreograms of extracts from three growth zones of broad bean root. With ascorbate oxidase (L-ascorbate: oxygen oxidoreductase, E.C. 1.10.3.3) one new fraction was found in the electrophoreograms of extracts from the maturation zone, electrophoreograms of α-glucan phosphorylase (α-l,4-glucan: orthophosphate glucosyltransferase, E.C. 2.4.1.1), amylase (α-l,4-glucan 4-glucanohydrolase, E.C. 3.2.1.1), sucrose glucosyltransferase (disaccharide glucosyltransferase, E.C. 2.4.1.7) and leucine aminopeptidase (L-leucyl-peptide hydrolase, E.C. 3.4.1.1) were identical for all three investigated growth zones. Ascorbate oxidase activity occurred in the same regions of electrophoreograms as peroxidase activity.
TL;DR: In this article, various methods for extracting nucleic acids from pollen were tested to find a suitable procedure for obtaining a pure preparation of nucleic acid without the use of ion exchangers.
Abstract: Various methods for extracting nucleic acids from pollen were tested to find a suitable procedure for obtaining a pure preparation of nucleic acids uncontaminated by polysaccharides and polyphosphates without the use of ion exchangers. Extraction was carried out with perchloric acid, potassium hydroxide, ribonuclease and deoxyribonuclease, sodium tetraborate, and combinations of these. In all fractions, residues of precipitates and residues of extracted pollen matter, the quantity of RNA, DNA, proteins and concomitants,i.e. Polysaccharides and polyphosphates, was determined. The purity of preparations was checked by means of UV-spectra. The criterion of nucleic acid purity was agreement between the nucleic acid amounts calculated on the basis of measurement of absorption in UV-region, orcinol reaction and content of phosphorus. It was found that in our material only a few methods would be applicable and with great limitation, because many polysaccharides and polyphosphates appeared in nucleic acid fractions.
TL;DR: In this article, the authors measured the intensity of water uptake by half of splitroot cuttings from Coleus blumeiBenth by potometrical method and found that α-naftylacetic acid (NAA) appeared to induce a smaller degree of reversibility than NAA.
Abstract: The intensity of water uptake by halves of splitroot cuttings fromColeus blumeiBenth was measured by potometrical method. Inhibition of the water uptake induced by application of high concentrations of sodium salts of α-naftylacetic acid (NAA) and 2-methyl-4-chlorophenoxyacetic acid (MCPA) in the treated half of the root induced an increase of water uptake by the other half. The compensation is not complete. If only one half of the root treated by synthetic auxin was allowed to take up water, MCPA appeared to induce a smaller degree of reversibility than NAA.