Showing papers in "China Journal of Modern Medicine in 2005"

Association between DNA polymorphism of human vitamin D receptor gene and type 2 diabetes mellitus

Abstract: To explore the relationship between DNA polymorphism of human vitamin D receptor (VDR) gene and type 2 diabetes mellitus within Chinese population. Among Han Chinese in Jiangsu province, 104 patients with type 2 diabetes mellitus and 77 nondiabetic subjects were collected randomly. By restriction fragment length polymorphism analysis, the alleles frequencies and genotype frequencies of VDR gene were compared between nondiabetic subjects and the patients of type 2 diabetes mellitus. The prevalence of VDR gene genotype frequencies and allele frequencies in patients with type 2 diabetes mellitus was significantly different from that in control subjects(χ2=9.993, P=0.007; χ2=7.698, P=0.006; respectively). The allele f and genotype ff were more common in patients with type 2 diabetes mellitus than in nondiabetic subjects, the relative risk (RR) was 3.9, P0.05. [Conclusion] Polymorphism of VDR gene is associated with type 2 diabetes mellitus in Han Chinese. Allele f may be a susceptible gene contributing to the development of type 2 diabetes mellitus in Han Chinese.

Construction of Bcl-2 shRNA expression plasmid and its inhibition on the growth of HL-60 cells

Abstract: [Objective] To construct expressing vector of short hairpin RNA( shRNA ) targeting Bcl-2,and investigate the effects of Bcl-2 shRNA on the growth of HL-60 cell line [Methods] Two of 66 base pairs oligonucleotides for short hairpin expression targeting the coding region of Bcl-2 mRNA were designed and were chemically synthesized Double DNA sequences were obtained through annealing Pgenesil-1 vector was linearized with BamHI and HindIII Finally, annealed oligonucleotides were inserted into the downstream of treated Pgenesil-1 vector U6 promoter to construct RNAi plasmid Oligonucleotide with a scrambled sequence was used as negative control Recombinant expression vector was identified by digestion with PstI and SalI and confirmed by sequencing analysis Bcl-2 shRNAs were transfected into HL-60 with Lipofectamine Cytotoxic effects were measured by use of MTT method The expression levels of Bcl-2 mRNA were assayed by RT-PCR [Results] Identifying by enzyme cutting and sequencing showed the insertion sequence was correct Furthermore, 66 bp had been inserted the expected site RT-PCR assay showed that the expression levels of Bcl-2 mRNA from HL-60 cells decreased after Bcl-2 shRNAs treatment There was no difference in Bcl-2 mRNA levels between control shRNA and untreated cells Viable cells at 48, 72 and 96 h after treatment with Bcl-2 shRNAs were less than that after treatment with control shRNAs and untreated HL-60 cells, respectively (P 005) [Conclusion] Bcl-2 shRNA expression vectors have been constructed successfully, could effectively inhibit the growth of HL-60 cells

Effect of angelica on proliferation of neural stem cells from rats'embryos in hypoxia model

Abstract: [Objective] To study the effect of Angelica injection on proliferation of neural stem cells(NSCs) from embryonic rats of hypoxia in the inner of the uterus. [Methods] The conceiving female rats were injected with Angelica injection through caudal vein before their embryos were in hypotonic hypoxia in the inner of the uterus. The expression of Nestin in brain of embryos was studied with immunohistochmistry, then the results were analyzed by image processing system. [Result] The colour of the immunohistochemical staining of Nestin in hypoxia group(HG) was darker than that of control group (CG) (P 0.05), and the quantity of postive cells in HG was more than that of CG (P 0.05). But in Angelica group (AG), them were lighter and less than that of HG(P 0.05). [Conclusion] Hypoxia can stimulate the proliferation of the NSCs. Angelica injection could play a protective role to NSCs from embryonic rats in hypoxia. The model of hypoxia in the inner of the uterus could be established by making the conceiving female rat in hypotonic hypoxia.

Calcium phosphate nanoparticles as a novel nonviral vector in cancer gene therapy

Abstract: ObjectiveTo study the biological characteristics of calcium phosphate nanoparticles and evaluate the promising in cancer gene therapy. Methods Calcium chloride modified nanoparticles prepared by means of chemistry. Analyse the combination of calcium phosphate nanoparticles and DNA, the protection to DNA as well by gelose gelatin electrophoresis. When the green fluorescence protein gene was regarded as report gene, gene vector of calcium phosphate nanoparticles transfected CNE- 2 cell in vitro and vivo. Combine the calcium phosphate nanopar- ticles and suicide gene yCDglyTK for Nasopharyngeal Carcinoma (NPC) therapy in vitro.ResultsCalcium phos- phate nanoparticles went into cell and could protect DNA after its combination with DNA by electro- microscopy. Regard calcium phosphate nanoparticles as vector of gene transfection, transduct green fluorescence protein gene in- to CNE- 2 cell, the green fluorescence protein high expression can be seen by fluorescence microsopy. The results of transfection in vivo showed that gene introduced by nanoparticles had a good expression in tumor tissue. The results of transducting suicide gene yCDglyTK by calcium phosphate nanoparticles for Nasopharyngeal Carcinoma (NPC) therapy in vitro indicated that large numbers of CNE- 2 cells were dead after the addition of 5- FC. ConclusionCalcium phosphate nanoparticles has great biological characteristics and it is one of promising vectors of gene trans- fection and gene therapy.

Analysis of down's syndrome screening and antenatal diagnosis of 3195 cases in the middle period of pregnancy

Abstract: [Objective] To explore the practical value of Down's screening in the middle period pregnancy in detecting chromosomal disease and congenital birth defect and predicting the outcome of pregnancy. [Methods] Serum AFP and free-β-HCG level of pregnant women(14~20+6 weeks) were detected by time-distinguished fluorescence immunoassay. Down's syndrome risk value was calculated with Multicalc software, its cut-off value was 1:250. The pregnant women whose value are higher than or equal to 1:250 would be at high risk and need to have the cordocentesis for fetal karyotype after 18 weeks. [Results] 3195 pregnant women accepted antenatal screening, 232 cases of them were detected at high risk, and positive rate was 7.3%,including 169 cases of high risk with Down's syndrome(fasle positive rate 5.2%). Fetal karyotypes of 147 cases diagnosed by the cordocentesis, 19 cases with fetal abnormal chromosome, the detectable rate was 12.9%,including three cases of Down's syndrome, a case of 18-trisome and a case of X-trisome. There is signification different in the rate of the abnormal outcome of pregnant women and fetuses in two groups of high risk and low risk of Down's syndrome (30.2% and 12.3% respectively, P 0.05). [Conclusion] The serum AFP and free-β-HCG level of pregnant women during second-trimester were effective indexes to screen abnormal fetus and predict outcome of pregnancy and fetus, which would be a practical method of preventing congenital defect.

Study on relativity of children obesity and metabolism syndrome

Abstract: [ Objective] To investigate the relativity of children obesity and some indexes of metabolism syndrome. [Methods] 69 volunteers with obesity were randomly selected as obesity group, 30 normal volunteers matching in sex and age as control group, out of 13,702 children and adolescents (2~18 years old) in Xiangtan city. Their index of body weight (IBM), blood pressure (BP), blood lipid (BL), hypersensitive C-reactive protein (hsCRP), leppin (LP), fasting blood glucose (FBG), fasting pure insulin (INS), blood uric acid (UA), and liver B-ultrasound were examined, and their mean artery pressure (MAP) and insulin sensitive index (ISI) were calculated on the basis of blood pressure and fasting pure insulin respectively, and then the differences of the indexes between two groups were compared and the relativity of the indexes was analyzed. [Results] The incidence of fatty liver, the values of MAP, triglyceride (TG), Apolipoprotein-B (Apo-B), hsCRP, INS, LP and UA were significantly higher in obesity group than in control group (P 0.01), while the values of Apolipoprotein-A (Apo-A) and ISI were significantly lower in obesity group than in control group (P 0.01). The value of hsCRP was significantly positively correlative to those of FBG and INS (P 0.05 or P 0.01), while it was significantly negatively correlative to that of ISI; the value of LP was significantly positively correlative to those of BMI and INS (P 0.01), while it was significantly negatively correlative to that of ISI; the value of INS was significantly positively correlative to that of BMI (P 0.01), while it was significantly negatively correlative to that of ISI; the value of UA was significantly positively correlative to those of BMI and INS (P 0.01), while it was significantly negatively correlative to that of ISI. [Conclusions] Insulin resistance (IR), LP resistance (LR), compensatory hyperinsulinemia, liver injury, hyperlipidemia, hypertension, hyperuricacidemia may exist in the children with obesity, and some inflammatory factors such as hsCRP may play an important role in pathogenesis of children obesity.

Study on cytotoxicity and oxidative effects of different sizes of hematite (Fe_2O_3) nanoparticles on CHL cell in vitro

Abstract: [Objective] To investigate the cytotoxicity among different size of hematite nanoparticles by 50% inhibitory concentration (IC50) and oxidative effects. [Methods] CHL cell was used in this study. Three sizes of Fe2O3 nanoparticles 8, 13, and 37nm were administered to the cell cultures with different doses and times as experimental groups. The IC50 and growth-inhibitory curves of cells were assayed by cell counting. The concentration of malondialdehyde (MDA) were determined by thiobarbituric acid reactive substance method and the activity of super oxide dismutase (SOD) was determined by xanthine oxidase method. [Results] When cells were exposed to three sizes of nanoparticles respectively within a dose range, the cells inhibitory (CI) rate decreased as the dose increasing with a relationship of dose-effect response, which however disappeared beyond the dose range. Within the range of dose-effect as above, we can calculate the IC50 of three sizes of nanoparticles: IC50(8 nm)=279.585 μg/mL, IC50(13 nm)=254.739 μg/mL, IC50(37 nm)=561.237 μg/mL. The hematite nanoparticles could induce the oxidative stress condition in CHL cells in relation to time, but no notable correlation between the index of MDA, SOD and the size and doses. [Conclusion] There is difference of cytotoxicity among different sizes of hematite nanoparticles. Meanwhile the hematite nanoparticles could induce oxidative stress condition in CHL cell, which maybe correlative to its cytotoxicity by time and effect relationship.

Effect of salpingectomy on ovarian response to superovulation in vitro fertilization-embryo transfer program

Abstract: To investigate the effect of salpingectomy on the funtion of ovary in patients undergoing an IVF-ET treatment cycle. The data of 45 cycles of IVF-ET, which were accomplished in the Reproductive Medicine Center of Third Affiliated Hospital, Guangxi Medical University during May 2000 and September2003, from 42 patients with history of unilateral salpingectomy clue to ectopic pregnancy, were analyzed reprospectively (study group). In the study group, mean ovarian volume, number of follicles and number of oocytes retrieved from each orary were assessed. The control group was 129 cycles of IVF-ET,which were accomplished in the same duration, from 121 patients with fallopian tube obstruction but without hydrosalpix. There were fewer follicles developed and oocytes retrieved in unilateral salpingectomy group, but no differences in other cycle characteristics between the study and control groups were found. Amony the patients who had undergone unilateral salpingectomy, significantly fewer follicles developed and consequently fewer oocytes were retrieved from the ovary on the operated side. There were also significantly small in mean ovarian volume during late follicle period. But there were no differenecs in mean ovarian volume during early follicle perild. [Conclusion] Salpingectomy has detrimental effects on ipsilateral ovarian reponse to superovulation. Salpingectomy may has detrimental effects on the blood supply of ipsiateral ovary. So we should try our best to reserve the salpinx during tubal pregnacy if the patients need to keep their reproductive capabilities.

Expression of KiSS-1 in human pancreatic cancer and relationship with their invasion and metastasis

Abstract: [Objective] To investigate the expression of KiSS-1 in human pancreatic cancer in relation to the clinical significance. [Methods] RT-PCR analysis and western blot analysis were used to detect the expression level of KiSS-1mRNA and its protein peptide-metastin in tissues of 37 cases of patients with pancreatic cancer, 27 cases of their metastatic site and 9 cases of normal pancreatic tissues. [Results] The expression of KiSS-1 in pancreatic tissues were significantly lower than those in normal (P 0.01, P 0.01). The expression of KiSS-1 had no correlation with sex, age, size of tumor and histological grade. However, the expression of KiSS-1 was related to the clinical stage, metastasis and nerves invasion significantly. The expression of KiSS-1mRNA and metastin were (0.135±0.121) and (9.23±2.74) μg /100 μg total protein separately in stage III and IV, which were significant lower than those in stage I and II (P 0.05, P 0.05). It showed a decreased tendency in pancreatic cancer without metastasis, with metastasis and metastatic site, which was lowest in metastatic site. The expression of KiSS-1 in tumor with nerves invasion were significantly lower than those without nerves invasion (P 0.01, P 0.01), which correlated coefficient (Kendall τ-b) were 0.597(P =0.003) and 0.438 (P =0.007). [Conclusion] The expression of KiSS-1 has close relation to invasion and metastasis of human pancreatic cancer, and may be involved in regulation of this process.

Elimination of oxygen free radicals in rat liver mitochondria by total saponins of panax notoginseng during ischemia reperfusion

Abstract: [Objective] To observe the elimination of oxygen free radicals in rat liver mitochondria by total saponins of panax notoginseng during ischemia reperfusion [Methods]To infuse and reserve PNS to rat liver though direct channel before blood blocked and then to gain rat mitochondria with ischemia reperfusion Determining the activity of SOD, MDA and the content of calcium, determining histologic changes by electron microscope [Results] After 90-minute reperfusion, the activity of SOD in the PNS group (10767±1638) NU/mgpro is significantly lower than that in control group (15945±2891) NU/mgpro (P 005) and the content of MDA (1822±0240) nM/mgpro is also lower than that in control group (2893±0782) nM/mgpro (P 005) More mitochondria injury was observed in PNS group than that in control group [Conclusion] PNS can protect rat hepatocyte from ischemia reperfusion injury by direct eliminating oxygen free radicals and boosting the activity of SOD, especially during early stage of ischemia reperfusion

Biocompatibility study of nano-hydroxyapatite artificial bone

Abstract: [Objective] To study the biocompatibility and safety of nano-hydro xyapatite artificial bone for the clinical use in the bone's reconstruction fiel d. [Method] Evaluated the biocompatibility through the experiments including a cute systemic toxicity test,pyrogen,intracutaneous test and long-term muscle and bone implant test. [Results] All results indicated that the material had no t oxicity,no irritation to skin and did not contain the pyrogen material. [Conclu sion] The nano- hydroxyapatite (nano-HA) artificial bone possesses excellent bi ocompatibility.

Retroviral-mediated high efficient expression of target gene in expanded rat mesenchymal stem cells

Abstract: [Objective] Investigate the expression and infection efficiency,infection time of retroviral delivery of genes in vitro by delivering target gene mouse homeobox gene Sox1 into mesenchymal stem cells(rMSC)with retroviral system.[Methods] The recombinant retroviral plasmid DNA carrying the Sox1 gene was constructed by subclone technique,then transfected to PT67 cells to package recombinant retroviral particles.rMSC was infected with the recombinant retroviral particles.pLEGFP-N1 containing enhanced green fluorescent protein gene(EGFP)expression cassette was used as a parallel matched control.Infection and expression efficiency were assessed by detecting the expression of EGFP,analysis of Western blot and immunohistochemistry.[Results] The recombinant retroviral vector pLXSN-Sox1 and made the high efficient expression of the retroviral particles Retro-Sox1.When infected by the retroviral particles,21% of the rMSC was positive to EGFP and last for 20 days.Only 3% of the rMSC was positive for EGFP in comparison group used routine method of lipofectamin mixed with plasmid DNA.Immunohistochemistry and Western blot showed that target gene Sox1 was expressed in infected rMSC.[Conclusion] Recombinant retrovirus high-effectively mediated gene transfer in expanded rMSC,with the stable expression of target gene.

Combination of transcatheter arterial chemoembolization and CT-guided Percutaneous ethanol injection therapy in treating advanced hepatocellular carcinoma

Abstract: Objective Assess the liver artery chemotherapy embolism (Transcatherter arterial chemoem- bolieation, TACE) The skill (Percutaneous ethanol injection therapy, PEIT) curative effect treated cancer of the liver is geted off after the hide the anhydrous ethyl alcohol of puncture cancer of the liver to inject in alliance CT' s boot.MethodsTo 64 instances after confirms in the interest of previoysly the quality the delivering cancer of the liver randomly separates into two series, Be living to be underway the intermission stage of TACE, 32 The instance is living the down PEIT of CT' s boot, moreover against the same term 32 instances walkes purely TACE compara- tively.ResultWalkes purely TACE series, 1 year, 2 year living is lead to act as 46.9% separately 12.5%, TACE ally PEIT series, 1 year, 2 year living is lead to act as 71.9%, 31.3% separately. The pure TACE of AFP descent scope series act as 56.3%, and TACE allys PEIT and series act as 78.1%.ConclusionThe TACE allys PEIT and actd as previously one kind of synthesis that the quality delivering cancer of the liver is better and treats the means, may lift cancer of the liver treats the curative effect.

Investigation of nosocomial infection of 1184 patients of geriatric surgery

Abstract: [Objective] To estimate the nosocomial infection(NI) and risk factors of geriatric surgical patients. [Method] 1184 senile patients who were hospitalized from January 2001 to December 2004 were studied by prospective and retrospective methods. [Results] Seventy-five of 1 184(6.33%) patients developed NI, the NI case rate was 6.84%. The most common infection site was lower respiratory tract (27.16%), then the surgical incision (22.22%), the upper respiratory tract (16.05%) and the urinary track (7.41%). NI were high in patients who were older than 75 years age, male, hospitalization longer than 10 days and those who had been operated or performed general anesthesia. [Conclusions] Patients of geriatric surgery, especially those who have been operated easily developed NI. Old age, sex, hospitalization time, invasive operation and anesthesia are important risk factors.

Guidance of activated carbon nanoparticles in gastric cancer lymphadenectomy

Abstract: To evaluate the clinical applied value of activated carbon nanoparticles to guide gastric cancer lymphadenectomy. 32 cases of gastric cancer received activated carbon nanoparticles injection around the tumor by preoperative submucosal endoscopic injection or intraoperative subserosal injection. The number of dissected lymph nodes, black-stained lymph node, positive lymph node and the side effect of the procedure were analyzed respectively. The average lymph nodes resected was higher in activated carbon(AC) group (32.7±11.4) than in control group (17.0±8.7), especially in N2 (23.1±8.0 ) than in N1(8.7±4.2). The total black-stained ratio of lymph node was 58.2%, with N1(66.7%) and N2 (49.8%) in AC group. The positive rate of lymph node was higher in black-stained lymph node of AC group (20.7%) than in unstained lymph node of AC group(3.7%) and control group(15.2%). The operative periods was slighted longer in AC group than in control group, with same operative complications. No serious side effect happened of activated carbon nanoparticles injection in local gastric tissue. [Conclusions] Local injection of activated carbon nanoparticles around the tumor is an effective, easy and safe procedure to guide gastric cancer lymphadenectomy.

Expression of HIF-1α and VEGF in non small cell lung cancer and relationship between them and tumor angiogenesis

Abstract: To investigate the relationship between the expression of HIF-1α, VEGF and tumor angiogenesis in non small cell lung cancer(NSCLC). The expression of HIF-1α, VEGF and MVD were evalulated by immunohistochemical method. The expression of HIF-1α and VEGF in NSCLC tissue was demonstrated in 71.11% and 66.7%, respectively, and significantly higher than that of normal lung tissue (P0.05); there was a statistically significant correlation between HIF-1αexpression and lymph node metastase, TNM stages,VEGF expression and MVD; But no significant corerelation was seen between HIF-1αexpression and tumor size, pathological grade,; there was also a statistically significant correlation between VEGF expression and tumor size, lymph node metastase, pathological grade ,TNM stages and MVD. [Conclusion] The present finding suggests that the expression of HIF-1α,VEGF correlates closely with tumor invasion and angiogenesis.

Study of effects of Verapamil on growth of meningioma

Abstract: To investigate the effects of verapamil on the proliferation of meningioma cells in vitro and in vivo. Verapamil with various concentration were put in the cultured meningioma cells and after 24 hours by means of MTT to value the proliferation of meningioma cells. The meningioma model was established by implanting the newly removed tumor fragments into the nude mice subcutaneously. The nude mice with tumors were divided into two groups. One group was treated with verapamil resolved in water and the other is a control group without verapamil. Tumors volume was measured and after 12 weeks the tumors were taken out and examined histologically. Proliferating cell nuclear antigen (PCNA) was detected with immunohistochemistry. Verapamil inhibits the growth of cultured meningioma cells in concentration dependant. The inhibition can be observed in the concentration of 1 μmol/L and the mostly obvious effects appear in the concentration of 100 μmol/L. Tumors volume of the verapamil treated group is obviously smaller than that of the control group(211.40±5.50 vs 163.94±3.62, P 0.01)and its expression of PCNA is also lower(1.52±0.24 vs 2.86±0.53). Tumor inhibition rate is about 22.45%. [Conclusion] Verapamil can inhibit the proliferation of meningioma cells in vitro and vivo.

Analysis of 29 cases of rare uterine angioletomyoma

Abstract: [Objective] To explore the clinical characters and clinical features of the uterine vascular leiomyoma. [Methords] Analysing the 29 cases that are pathologically diagnosised uterine angioleiomyoma, from the particular features including physical examination, seeing in the operation, pathological change and the analysis about prognosis. [Results] The tumor diameter and blooding in operation of the vascular leiomyoma have great differences with myoma of uterus (P 0.05). The certain diagnosis before operation is difficult. some cases could be existent together with cellular leiomyoma. We haven′t found recurrence and transfer in random visit after cure. [Conclusions] Vascular leiomyoma have characteristics in clinic. On the one hand the tumor diameter is larger; On the other hand, there is mass of blooding in operation, but the outcome is satisfactory.

Clinic feature and risk factors of type 2 diabetic nephropathy

Abstract: To investigate the clinical features of type 2 diabetic nephropathy and analyze the risk factors of affecting incidence in order to achieve better prevention and treatment. A retrospective analysis of clinical features of diabetic nephropathy was conducted based on 511 cases in our department from 1997 to 2001. They were 175 diabetes mellitus without nephropathy, 131 early stage diabetic nephropathy, 133 diabetic nephropathy with overt proteinuria, 72 end-stage diabetic nephropathy. Serum levels of lipid, glucose, urea nitrogen, creatinine, uric acid, and blood pressure, albuminuria were monitored in the four groups, and their level were compared. Age, course of disease, hypertension, hyperlipidemia, hyperuricemia, coronary heart disease, biliary calculus, diabetic retinopathy, diabetic peripheral neuropathy, diabetic foot, systolic and diastolic blood pressure were significantly different among four diabetic subgroups (P 0.05). Course of diabetes, systolic blood pressure and hyperlipidemia had significant positive effect on the incidence of diabetic nephropathy (P 0.05). [Conclusion] Dissatisfaction control of blood pressure, blood sugar and lipid result in the progression of diabetic nephropathy. Diabetic nephropathy is closely associated with other vascular complications. Inspecting urine albumium excretion rate periodically, controlling blood pressure and blood sugar strictly and correctting dyslipidemia is an effective way of better prevention and treatment of diabetic nephropathy.

Literatures analysis of tracheal adenoid cystic carcinoma about 5 cases and other domestic 131 cases

Abstract: 【Objective】Increase the knowledge about tracheal adenoid cystic carcinoma (TACC).【Methods】Review 5 cases with tracheal adenoid cystic carcinoma in Changhai Hospital during 1995￣2003 and search for 131cases in internal periodicals.【Results】The main manifestation of TACC is cough, stridor and progressive inspiratorydyspnea. It is easy to confuse with bronchial asthma and chronic bronchitis. The operative treatment is 122 cases,Putting stents in trachea is 6 cases, interventional treatment by fiberoptic bronchoscopes is 3 cases, and local radio-therapy is 47 cases. The death is 20 cases.【Conclusion】The clinical symptoms of TACC is not typical. The misdi-agnosis rate is high. The best treatment is as soon as excision and radiotherapy. The better palliative treatment is byfiberoptic bronchoscope′s interventional treatment and can improve the patients living quality.

Study on Expression and Distribution of Macrophage Inflammatory protein-1α in Atherosclerotic Tunica Intima of Artery from Type 2 Diabetics by Using Immunohistochemistry

Abstract: 【Objective】 To detect the expression and distribution of macrophage inflammatory protein-1α (MIP-1α)in atherosclerotic tunica intima of artery from type 2 diabetics and non-diabetics in order to try to find out the effect of MIP-1α on the formation and development of atherosclerosis in type 2 diabetics. 【Methods】 Use the immunohistochemistry method to detect the expression and distribution of MIP-1α in atherosclerotic tunica intima of artery from 13 type 2 diabetics, 15 non-diabetics and normal tunica intima of artery from 7 healthy people, respectively. Then computer image analyzer was used to relative quantu analysis. 【Results】 MIP-1α was absent in normal arteries with average gray value of (234.27±8.04). There was different expression of MIP-1α in different-stage tunica intima of artery from non-diabetics with atherosclerosis: With the progression of atherosclerosis, the immunoreactivity of MIP-1α increased with average gray value of (168.40±7.69) in fatty steaks and (173.67±6.56) in fibrous plaques. MIP-1α was mainly in endothelial cells and foam cells during the stage of fatty steak, while MIP-1α was mainly in foam cells and there was little expression in endothelial cells during the stage of fibrous plaque. Average gray value of MIP-1α in fatty steaks from type 2 diabetics was (132.73±6.01). There was a significant difference between average gray value in fatty steaks from non-diabetics and average gray value in fatty steaks from type 2 diabetics (P＜0.05). Average gray value of MIP-1α in fibrous plaques from type 2 diabetics was (138.68±9.41). There was a significant difference between average gray value in fibrous plaques from non-diabetics and average gray value in fibrous plaques from type 2 diabetics (P＜0.05). There was strong expression of MIP-1α in endothelial cells and foam cells during two stages of type 2 diabetics. 【Conclusions】 MIP-1α is absent in normal arteries. Expression of MIP-1α in fatty steaks from non-diabetics increases obviously, but expression of MIP-1α in fibrous plaques from non-diabetics decreased gradually. Expression of MIP-1α in fatty steaks from diabetics increases significantly and there is a significant difference between average gray value in fatty steaks from non-diabetics and average gray value in fatty steaks from type 2 diabetics (P＜0.05). There is still strong expression of MIP-1α in fibrous plaques from type 2 diabetics and there is a significant difference between average gray value in fibrous plaques from non-diabetics and average gray value in fibrous plaques from type 2 diabetics (P＜0.05).

Effects of testosterone on left ventricular function of male rats with chronic heart failure

Abstract: [Objective] The purpose of our present study was to examine the effect of Testosterone on the left ventricular structure and function of male rat with chronic heart failure (CHF) [Methods] 74 SD male rats were divided into 4 groups: Control; CHF; Orchiectomy(ORX)+CHF; ORX+CHF +Testosterone(T) The left ventricular structure and function were examined by echocardiography [Results] In ORX+CHF male rats, heart function greatly decreased companying with the decrease of serum testosterone level T administration led to myocardial hypertrophy and increased heart weights T treatment decreased left ventricular end-diastolic dimension (EDV) and prevented the decline of fractional shortening (FS) [Conclusion] In male rats with chronic heart failure, the decrease of serum testosterone level deteriorated the heart function and testosterone treatment can improve left ventricular systolic function

Clinical analysis of 235 cases of preterm labor

Abstract: [Objective] To discuss the main risk of premature delivery[Methods] To study the main risk factors we collected 235 cases of premature delivery[Results] Premature rupture of membrane,fetal distress,severe pregnancy induced hypertension,placenta praevia were the main risk factors of premature delivery[Conclusion] Early prenatal care that identifies and treats risk factors of preterm labor and the appropriate management decision for the patient,can optimize pregnancy outcome

Effect of salvia miltiorrhizae injection on apoptosis of endothelia cells induced by arecoline and the aetivity of caspase-3

Abstract: [Objective]To investigate the effects of Salvia Miltiorrhizae (SM) on the apoptosis of vascular endothelia cells(VEC) induced by arecoline and the activity of Caspase-3. Human Umbilical Vein Endothelial Cells(HUVECs) were cultured in vitro. Apoptosis of VEC were induced by arecoline, and SM was added in test specimen. Apoptotic morphological changes and percentage of apoptosis were observed under fluorescent microscope. The activity of Caspase-3 were evaluated by the colorimeteric assay. Compared with control, VEC apoptosis increased significantly and the activity of Caspases-3 increased in arecoline(90ug/ml) group. SM(8mg/ml) reduced the apoptosis induced by arecoline (P 0.05) and the activity of Caspases-3. [Conclusion] SM could downregulate the arecoline-induced apoptosis of VEC and maintain integrity of endothelium, suggesting that SM may posses antifibrotic ability.

Effects of jianlin decoction to aged hypertension patient's life mass and CAT

Abstract: [Objective] To observe the effect of jianlin decoction to blood pressure and system of resist oxidation in the hypertension of liver yang excessive patients. [Methods] The 60 patients with hypertension were divided two groups: in one group( jianlin decoction group), the patients were treated by jianlin decoction, in the other( felodipine group), the patients were treated by felodipine. Before and after four weeks, we measure the life mass and serum CAT, at the same time, we measure the CAT in 25 normal person. [Result] After 4 weeks, the grade of life mass and serum CAT increased in both groups(P 0.01), but the jianlin decoction group's increased more distinctly (t =15.94, 0.01). [Conclusion] Jianlin decoction can improve the life mass and increase the serum content of CAT in the liver yang excessive patients, thereby, preventing lipid supper oxidation in endothelium cell, delay caducity.

Expression and significance of human mismatch repair gene (sMSH2) and proliferating cell nuclear antigen (PCNA) in lung cancer

Abstract: To investigate the expression and significance of human mismatch repair gene (hMSH2) and proliferating cell nuclear antigen (PCNA) in lung cancer. Expression of hMSH2 and PCNA in 56 cases with lung cancer was examined by S-P immunohistochemical staining. In 56 cases with lung cancer, the positive expression rate of hMSH2 was 28.6%. The positive rate of Hmsh2 Expression in the patients with high and middle differention grades was significantly higher than that of the others (P0.01), meanwihile, the positive rate of cases with lymph node metastasis (P0.05). It wasn′t positively correlated with pathological types. In 56 cases with lung cancer, PCNA labeling index (PCNA-LI) in the patients with high and middle differention grades was lower than that of the others (P0.01), meanwhile, the PCNA-LI of patients with lymph node metastasis was significantly higher than that of patients without metastasis (P0.05). PCAN-LI in hMSH2 positive lung cancer tissue was higher than that in hMSH2 negative tissue (P0.01). [Conclusions] The abnormal expression of hMSH2 and PCNA may be involved in the carcinogens process of lung cancer and correlated with differentiation grades and lymph node metastasis.

Establishment of local cerebral ischemia animal models in monkey by autologous pale thrombus

Abstract: [Objective] To discuss the function about autologous blood clots in making the animal model of cerebral ischemia. [Method] Authors transmitted autologous blood clots into M1 branch of middle cerebral artery (MCA) of monkey by endovascular tiny injured repair. [Results] The M1 branch of MCA developed not enough clear by digital subtraction angiography (DSA) after embolizated. One hour later, acute local ischemia focuses were seen by CT filling angiography and lower density old local ischemia focuses were seen 72h later using CT noncontrast scan. The two dead animals were immediately anatomized and cerebral TTC staining was found positive. The living animals had various degree behavior obstacle after coming to their senses. [Conclusion] Establishment of this model is successful. It has fairly important value for the studying of stroke, especially in screening out potent thrombolytic drugs.

Determination of concentration of levodropropizine in plasma by RP-HPLC with fluorescence detector

Abstract: [Objective] To establish a RP-HPLC method for determining levodropizine. [Method] Levodropizine was extracted from blood samples. Determination of levodropizine were carried out using a HiQsil C18 (150×4.6 mm, 5 μm) column at 30℃. the mobile phase consisted of 0.1M KH2PO4:acetonitrile:methanol (82:3:15). The flow rate was 0.6ml.min-1.The wave length of fluorescence determination: Ex=240 nm, Em=350 nm. [Result] Levodropizine could be separated well. The calibration curves of levodropizine in the range of 3.46~886 ng/mL were linear. The minimal quantified concentration of levodropizine was 3.46 ng/mL. The relative recovery were 106.12%~109.05%. The absolute recovery of levodropizine from plasma were more than 80%. The RSD of inter-day and intra-day were less than 10.0%. [Conclusion] The method is accurate and sensitive. It is suitable to be applied in thetheraputic drug monitor of levodropizine.

Improvement of sleep structure in patients with obstructive sleep apnea hypopnea syndrome treated by auto-continuous positive airway pressure

Abstract: [Objective] To observe the efficiency of auto-continuous positive ai rway pressure(Auto-CPAP) treatment in patients with obstructive sleep apnea hypo pnea syndrome(OSAHS). And to investigate the influence of sleep structure in OSA HS patients treated by AUTO-CPAP. [Methods] The polysomnography (PSG) were tak en in 28 patients with OSAHS untreated previously. Their parameters of PSG obtai ned before and during the AUTO-CPAP treatment were analyzed. [Results] During the AUTO-CPAP treatment the longest apnea time had been shorten (25±12.9) s vs. (57.6±18.3) s, P 0.001. Apnea Hypopnea Index(AHI) had been decreased(8.0±8.7 )/h vs. (54.4±22.7)/h, P 0.001. The lowest oxygen saturation had been raised ( 83.7±6.5) % vs. (66.0±12.1) %, P 0.001. And the average oxygen saturation imp roved too (93.6±2.2)% vs. (86.5±6.8)%, P 0.001). The deep sleep (sleep stage 3 and 4) improved (11.4±5.4)% vs. (5.4±8.0), P 0.01. Microarousle Index (MAI ) decreased from (31.5±19.5)/h to (8.3±5.1)/h (P 0.001). Sleep efficiency get a little lower (85.0±11.8) % vs. (89.8±9.1) %, P 0.05. [Conclusions] Auto- CPAP can improve the sleep apnea/hypopnea and noctural oxygendesaturation. The s leep quality of OSAHS patients improved too during treating.

Analysis of mistake diagnose and treatment for nasopharynx cancer

Abstract: 【Objective】To reduce mistake diagnosis and improvement early diagnose for nasopharynx cancer, thecauses of mistaken diagnosis were analyzed 【Method】During 1991￣2003, all mistaken diagnose patients with na-sopharynx cancer were analyzed 【Results】It was usual that nasopharynx cancer were taken for tumor of neck, para-pharyngeal tumor, strabismus, psychopathy, face or vocal paralysis, trigeminal pain, deviation of nasal septum, ade-noid hyperprophy and chronic rhinitis, etc 【Conclusions】The cause of mistaken diagnose for nasopharynx cancerare that its position is concealment, and haven’t special symptoms in early stage Considering to the diversity of na-sopharynx cancer symptom, careful examination to nasopharynx and biopsy are the primary measurement of decreasemistake diagnose to nasopharynx cancer