Showing papers in "Clinical Biochemistry in 1985"

TL;DR: The detailed procedure of a new sensitive fluorometric assay for human serum monoamine oxidase (MAO) with kynuramine as substrate is described, which is much simpler and more sensitive than other methods conventionally used.

TL;DR: Cross-reactivity studies utilising fluorescence polarization immunoassay have shown that cortisone is the most potent immunoreactive substance of cord serum, and the results suggest that the pump inhibitor is not progesterone.

TL;DR: An ion-exchange chromatographic procedure is described which facilitates the determination of beta-chain aminoterminal modified glycated hemoglobin and various abnormal hemoglobins were studied for their effect on the assay.

TL;DR: Various methods of quantitation of B12 reviewed in this communication include: microbiological, radioisotopic, high-performance liquid chromatography (HPLC), and the most recent radioimmunoassay (RIA) techniques are reviewed to provide awareness of the limitations of these methods.

TL;DR: Both methods were optimized to yield accurate values for normolipemic and slightly hyperlipemic serum samples and can be achieved by a combination of immunoprecipitation and polyanion precipitation, enabling the direct measurement of LDL cholesterol in the supernatant.

TL;DR: C covariance analysis demonstrated that age does not play an important role in influencing copper and Cu/Zn ratio and a decreased liver synthetic function, at least in part age-related, seems to be an additional factor in decreasing serum zinc values.

TL;DR: Comparing the two groups of acute pancreatitis patients after 6 days of treatment with gabexate mesilate, the authors observed a statistically significantly lower alpha-amylase activity in the serum of treated patients compared with the placebo group.

TL;DR: This study has shown that the proportion of picric acid, NaOH and serum have only a slight effect on the interference; the addition of a buffer to the NaOH solution increases the interference due to cephalothin and cEPhaloridine, but lessens that due tocefoxitin.

TL;DR: The present study demonstrates the stability of the QC material, defines the relative accuracy of the receptor assays, and provides estimates of within-batch and between-batch precision of the receptors assays.

TL;DR: This assay was used to analyze cerebrospinal fluid from 22 patients with severe head injury, 61 other cases of various neurological disorders, and 106 normal controls and showed that closed head trauma caused moderate to severe elevations in CSFMBP, and elevated CSF MBP was detectable in several diseases which involve CNS myelin.

TL;DR: Only rarely does drug interference with a method lead to a clinically significant change in a measured value; the magnitude of the change may relate linearly or non-linearly to the drug concentration but is usually independent of the target analyte concentration.

TL;DR: In 82 renal transplant recipients, the excretion of urinary alanine aminopeptidase activity daily after transplantation until patients' discharge from hospital was measured to measure diagnostic sensitivity, specificity, efficiency and likelihood ratios with regard to diagnosis of acute rejection crises.

TL;DR: The data suggest that stool chymotrypsin measurement is a simple and reliable "tubeless" test for the evaluation of exocrine pancreatic insufficiency in children with cystic fibrosis.

TL;DR: A simple, rapid and sensitive radioimmunoassay for plasma arginine-vasopressin (AVP) has been developed for routine use and validated by determinations of plasma AVP levels in rabbits and hamsters in various conditions.

TL;DR: Plasma prolinase activity was strongly dependent upon cytolysis because of the high activity in liver and the low activity in plasma, contrary to prolidase (EC 3.13.4.9) activity.

TL;DR: In children and adolescents over 10 years, there was considerable overlap in DHAS values between groups of children classified by pubertal stage, resulting from the dissociation between adrenal and gonadal development during normal puberty.

TL;DR: Serum immunoreactive alanine-glyoxylate aminotransferase (Im-AGT) was measured by enzyme-linked immunoadsorbent assay (ELISA) and the difference between acute and chronic hepatitis was not statistically significant.

TL;DR: Accuracy, reproducibility and linearity of the BCP albumin method on the aca are acceptable for clinical use and are shown to be similar to the immunological and rapid BCG methods.

TL;DR: The clinical presentation with progressive dystonia and dislocated lenses in an infant should suggest further laboratory investigations for this disorder which would not be detected by conventional laboratory screening procedures.

TL;DR: A colorimetric method was developed for the determination of nonenzymatically glycated albumin and adapted to a Flexigem centrifugal analyzer and correlated well with a thiobarbituric acid assay but less so with those for glycated hemoglobin.

TL;DR: Based on the homogeneity of the intraindividual variance, it is possible to show the level of minimal decrease in enzyme activity which constitutes a departure from "normal".

TL;DR: The results from this study indicate that a random urine sample should be as good as a 24-hour collection for diagnosis and follow-up of neural crest tumors.

TL;DR: In responders to chemo- and/or radiotherapy, the carboxypeptidases decreased and the angiotensin-I-converting enzyme increased to normal enzyme values, and in non-responders to these therapies no change in these enzymes could be observed.

TL;DR: It is suggested that a transient elevation of CSF β 2 -microglobulin may occur after intrathecal methotrexate and irradiation therapy, and evidence of CNS involvement was found in any of these patients.

TL;DR: Its consistent presence in tumours and in normal colon, contrasted to its variable appearance in serum, implies that there are other factors affecting its release into, or removal from, the circulation.

TL;DR: A rod IEF method is described, which allows the simultaneous quantitation of glycation on alpha and beta globin chains, which indicates that valinic or lysinic glycation have roughly the same clinical significance.

TL;DR: Of the enzymes studied, lipase, trypsin and pancreatic isoamylase are equally suitable for the evaluation of function in severe chronic pancreatitis, but not for the early diagnosis of the disease.

TL;DR: Comparison of results by two manufacturers' kit methods confirmed Bio-Rad's claim to increased low-end sensitivity of standard curve in range of clinical interest.

TL;DR: The HPLC analysis of sulfatide is improved by the use of a sulfated internal standard, sulfatoxymonoalkylmonoacylgalactosylglycerol, and there is no increase in plasma sulfatides compared to controls in the age group of MLD patients which the authors studied.

TL;DR: In blood samples taken from patients suffering from acute inflammatory processes the mean ZPP level was twofold higher, and the mean FPP was four times higher, than the normal level, suggesting better sensitivity of the FPP measurement for the detection of minor influences on the hemopoietic system.