Showing papers in "European Journal of Cell Biology in 1982"

TL;DR: It is indicated that primary cultures of neonatal rat aortic smooth muscle cells remain differentiated in culture and have the unique capacity to continue to synthesize and deposit large amounts of insoluble elastin which aggregate and from elastic fibres in vitro.

TL;DR: The sensitivity of detection of proteins by immunolabelling embedded and sectioned material depends upon retention of antigenicity during tissue processing, and losses in pea seed storage protein antigenicity have been assessed using a solid phase radioimmunoassay.

TL;DR: Results show that fatty acids are bound to a number of membrane-associated proteins, both glycosylated and unglycosylation, via linkages that resist purification of the proteins on SDS-polyacrylamide gel electrophoresis and are suggestive of covalent attachment of fatty acids to these proteins.

TL;DR: The Golgi apparatus and endoplasmic reticulum do not rearrange dramatically during the phenotypic conversion, although the locomotory cells are characterized by a zone close to the leading lamella, which is completely free from these organelles.

TL;DR: This result is significant, since it clearly demonstrates that the kinetochores are attached to a spindle component other than microtubules, and suspects that this matrix is contractile and part of the mitotic machinery for moving chromosomes.

TL;DR: In cells rapidly frozen and then dried from the frozen state, there is less evidence of shrinkage and probable change in the trabecular structure than in cells first fixed with glutaraldehyde and then frozen dried.

TL;DR: The results indicate that the heterogeneity of hepatocytes as to phosphoenolpyruvate carboxykinase content is due to a different expression of the genome.

TL;DR: Electron microscopy on serial sections has been used to compare the number and position of centrioles and processes in four neuroblastoma clones and in primary dorsal root ganglion neurones, suggesting that multiple centriole are not obligatory for multiple process formation.

TL;DR: A zymogen granule fraction has been isolated from rat pancreas, and its purity has been assessed by biochemical and morphological criteria and confirmed the absence of mitochondria, lysosomes, and rough endoplasmic reticulum fragments.

TL;DR: It is shown that marked changes take place in the organization of the intermediate filament system and in the contractile apparatus, both in atria and in ventricles of the rat heart during postnatal development.

TL;DR: It is suggested that the resultant modification of the intracellular environment via the hypertonic stress promotes the activation of centriolar 'information-bearing-residues' or 'seeds' located at the nuclear envelope.

TL;DR: The presence of presumptive kinetochore microtubules clarifies earlier reports on this group of fungi, but their unusual behaviour supports previous suggestions about the primitive nature of the spindle.

TL;DR: The method for electron microscopy of the Ag-staining of NOR-specific proteins was adapted to tissue sections from solid organs and it was concluded that in interphase nucleoli the Ag -staining NOR proteins are associated with the fibrillar components and with the derived nucleolar fibrillsar remnants.

TL;DR: Results show that a hyperthyroid state for 5 to 10 days induces a new peroxisome population in the liver of male rats, and it is discussed thatPeroxisomal metabolism may contribute considerably to the late biochemical effects of thyroid hormones, that means enhancement of lipid oxidation and heat production.

TL;DR: It could be shown that LDL preferentially binds to certain microdomains of the plasma membrane, the so-called coated pits, in this correlative study, employing thin sectioning, freeze-fracture and deep-etching techniques in conjunction with the polyene antibiotic filipin.

TL;DR: An independence of the cycle of microtubule-mediated events from other cyclical processes during the first cell cycles is demonstrated during thefirst cell cycles of sea urchin eggs at fertilization.

TL;DR: The structure of the membrane limiting apparently newly formed autophagic vacuoles was studied in vinblastine induced autophagocytosis in mouse liver parenchymal cells and in Ehrlich ascites tumor cells and it is apparent that the membranes of endoplasmic reticulum or Golgi apparatus are not directly involved in autophatic vacuole formation.

TL;DR: The simultaneous addition of alcian blue and tannic acid to human blood platelets during glutaraldehyde fixation leads to an excellent representation of coated membranes (CM) in platelets stimulated with ADP.

TL;DR: The results indicate that myosin in smooth muscle cells exists as thick filaments at any stages during contraction-relaxation cycle.

TL;DR: It was found that the development of bile canaliculi is characterized in its very early stage by huge accumulations of filipin-cholesterol complexes located at distinct domains of the contiguous membrane, which suggest a crucial role of cholesterol-rich membrane domains during initiation of a biliary polarity.

TL;DR: Besides the formation of protein bodies by the ER a second route of origination from vacuoles is proposed for Vicia faba seeds at early developmental stages.

TL;DR: The results indicate that both normal and FH fibroblasts internalize CF exclusively by coated pits, and adsorptive uptake mechanism that apparently is nonspecific but clearly cation-selective.

TL;DR: The nuclei of dinoflagellate protists display several distinctive features which make it difficult to assign these organisms as either eukaryotes or prokaryotes, and composition analysis of different fractions of the CsCl gradient revealed that the unusual pyrimidine, 5-hydroxymethyluracil, was not uniformly interspersed with thymine in the DNA.

TL;DR: Results indicate that the presence of the endosymbiote changes the lectin-binding sites at C. deanei surface membrane.

TL;DR: It is concluded that DNA replication is a prerequisite for its transcription by RNA polymerase B and the sequential replication of the genome of Physarum dictates the order of transcription during S-phase which may in part control the proliferative mitotic cycle ofphysarum.

TL;DR: It is suggested that the stalk particles found on the surface of chromaffin granules represent the F1-complex of the proton-pumping ATPase of these organelles.