Showing papers in "Experimental Eye Research in 1969"

TL;DR: In this article, a method for the isolation of the polysaccharides of bovine corneal stroma has been developed, based on chromatography on ECTEOLA columns.

TL;DR: A study has been made of the carbohydrate and oxygen metabolism of the rabbit cornea in vitro and it was found that exogenous glucose provides 90% of the substrate supply, the remainder being comprised chiefly by glycogen and endogenous glucose.

TL;DR: In the aging and cataractous human lens, soluble protein decreases and insoluble protein increases in concentration, and this change is more marked in the cataracts than in the aging normal lens.

TL;DR: In this paper, two experimental approaches have been used to determine whether the internal fibres of the amphibian lens have intact membranes, one involves an analysis of the potential transients obtained when potassium is substituted for sodium in the external medium and the other involves a correlation of lens electrical resistance with depth of penetration of the measuring electrodes.

TL;DR: The early effects of topical administration of 2% l-epinephrine bitartrate on the dynamics of aqueous humor were studied in vervet monkeys and it is suggested that epinephrine has a facility reducing effect that is due to relaxation of the ciliary muscle and a facility increasing effect that may be due to some change in the endothelium of the canal of Schlemm.

TL;DR: This growth resembled that noted previously when corneal burns were produced centrally to implanted tubes open at both ends, and suggested that the chemical vaso-stimulating factor may be a biogenic amine.

TL;DR: In mice carrying the dominant gene Cotaract-Fraser (or Shrivelled) pyknosis of the nuclei of the lens fibers is followed by cytoplasmic abnormalities and eventually complete destruction of the main lens fiber mass, leading to anterior polar cataracts.

TL;DR: A collagenase that can digest both collagen fibers in the stroma and the native collagen molecule was found in rabbit corneal epithelium, using a tissue culture assay.

TL;DR: Pigment epithelium microsomes are capable of catalyzing oxidation of NADH and NADPH in the absence of l -ascorbate, and the microsome-bound hemoprotein identified with P-450, a CO-binding hemop protein originally found in liver microsome is identified.

TL;DR: The attachment between the sensory retina and the underlying tissue in the ora serrata was found to be very strong; continued pulling resulted in stretching and ultimate tearing of the tissue without achieving an actual separation.

TL;DR: In this paper, a constant field theory was applied to the unidirectional flux data, and the ratio PNa/PK was found to be 0.07, where PNa is defined as the ratio of the current passing through the lens to the passive movement of the ions.

TL;DR: It was concluded that, in the process of transformation of stromal cells to dermatan sulfate-producing cells, injury to Descemet's membrane leading to impaired endothelial regeneration might play an important role.

TL;DR: From an analysis of the total cation and mobile anion content of the lens it seems likely that there are at least 100 m m of fixed negative charges within the lens, which would cause the internal osmolarity to exceed that of the aqueous humor causing the lens to swell.

TL;DR: Results of various methods for extracting whole lens indicate that the disulphide links of that part of the water-insoluble protein (albuminoid) that is also insoluble in urea are formed by oxidation with atmospheric oxygen.

TL;DR: All procedures converge in indicating that active transport of chloride can occur across the ciliary epithelium in the direction stroma→posterior chamber and equations derived from considerations of irreversible thermodynamics were used to obtain estimates of the relative contributions of Na+ and Cl− ions to the total short-circuit current.

TL;DR: Crystallins, FISC, and several longline crysallins are a family of heteropolymers whose mobilities are related to their subunit structure and each class appears to be a population of molecules in dynamic equilibrium with their subunits.

TL;DR: The glycosaminoglycan content of corneal stroma during transient edema produced by removal of the endothelium was measured and it was observed that this process is associated with a considerable loss of polysaccharides.

TL;DR: Atropine (3 μg/ml) did not significantly change the rate of aqueous humor production, the intraocular pressure or the recipient venous pressure, and Pseudofacility was not appreciably influenced by atropine.

TL;DR: The flux of glucose and methylglucose out across the layer is faster than would be expected on the basis of their size, suggesting that their exchange is facilitated.

TL;DR: The finding of uniform agranularity of agranular cholinergic vesicle in axons within the same nerve bundle, which could contain other axons bearing predominantly granulated vesicles, taken together with pharmacologic proof that these latter are adrenergic suggests a dual innervation to the iris dilator muscle.

TL;DR: The glycosaminoglycan pattern in human corneas afflicted with diseases was measured and in maculae corneae, keratitis and lattice degeneration a decrease in the polysaccharide content was observed.

TL;DR: These anchoring filaments appear to attach the lymphatic endothelium to the collagen fibres of the surrounding tissue and give the appearance of being under tension in the bulbar conjunctiva of rats and rabbits.

TL;DR: The antero-posterior distribution of sodium and potassium was determined in normal and refrigerated ox lenses and this results provide evidence in favor of both the “pump-leak concept” and the extracellular distribution of Sodium in the lens.

TL;DR: Care should be taken in the choice of anesthetic agent for use in experiments on intraocular pressure and gross facility in cats anesthetized with pentobarbital, urethane, chloralose/urethane and halothane, it is concluded.

TL;DR: The potassium efflux is not sensitive to short-term exposures to solutions containing 2,4 dinitrophenol (DNP) or ouabain, and this confirms that the potassium ions move outwards passively across the membranes.

TL;DR: Soluble lens proteins from five species of amphibia have been studied by zone electrophoresis and other immunochemical methods and the γ-crystallin appears to be the predominant lens protein in all the species.

TL;DR: Water permeability of Descemet's membrane can be calculated from the time sequence of corneal thickening, based on the theory of water imbibition by swelling membranes.

TL;DR: The Symposium was devoted primarily to the biochemistry of the proteins of the lens, and the chemical and physiological structure, the immunological reactions of the water soluble and water insoluble lens proteins was discussed.

TL;DR: Measurements of lactate production at steady state in aerobic conditions show conclusively that aerobie glycolysis does take place in the intact corneal epithelium of the ox, consistent with the existence of a higher concentration of lactates in the epithelia than in the stroma.

TL;DR: The retina of Poromitra nigrofulvus (Garman) has been studied by optical and electron microscopy and a system of membrane bounded lamellae permeating the retina from the outer nuclear layer to the inner limiting membrane is described.