Showing papers in "Indian Journal of Pharmaceutical Sciences in 1992"

Quantitative determination of bacosides by UV-spectrophotometry.

Abstract: Spectrophotometric method for quantitative determination of total Bacosides as Bacoside-A (the active constituent) - a mental retention capacity enhancer is reported.

Cassia fistula seed galactomannan : potential binding agent for pharmaceutical formulation.

Abstract: The water soluble gum isolated from the seeds of Cassia fistula Linn. found in abundance throughout India, has been evaluated for its binding properties for formulations of tablets containing 2 and 4 percent of gum. The binding properties of gum has been evaluated in relation to the conventional binders like Gum arabic, Gum tragacanth, Sodium CMC and Gelatin at different parameters like percentage of fines, tablet hardness,disintegration time and friability. Thr gum showed overall superiority in viscosity and binding properties as compared to other binders. The addition of preservatives effectively checked the gum's characteristic of losing viscosity on keeping. Like other binders,increase in concentration of gum from 2 to 4 percent, decrease the percentage fines (5.6 to 3.45), increase the hardness (kg/in2)5.11 +/- 0.7270, C.V.14.22 percent to 5.98 +/- 0.6863, C.V.11.47 percent)and disintegration time (30 min 29 sec to 36 min 44 sec). Percentage friability has been found to be almost equal (0.81 and 0.84) in Cassia gum although it was increased in other samples except gum tracanth. The results indicate that C.fistula seed gum is a potential binder for pharmaceutical industry.

Potential anticancer activity of saraca asoca extracts towards transplantable tumours in mice.

Abstract: The anticancer principle from Saraca asoca flowers indicated 50 percent cytotoxicity (in vitro) in Dalton's lymphoma ascites and Sarcoma-180 tumour cells at a concentration of 38 mug and 54 mug repectively, with no activity against normal lymphocytes but preferential activity for lymphocytes derived from leukemia patients (ALL). Tritiated thymidine incorporation studies in dicate the mechanism of action of the agent at the site of DNA synthesis.

Spectrophotometric methods for the determination of dilitiazem in pharmaceutical preparations.

Abstract: Two spectrophotometric methods for the determination of Diltiazem in Pharmaceutical preparations are described. The methods are based on the formation of coloured complex of the drug with cobalt-thiocyanate and methyl orange. The coloured species were extracted into organic solvents and measured at the wavelengths of maximum absorbance at 630 nm and 420 nm with cobalt and methyl orange respectively. Both the methods shows linearity in the range 60-600 mug/ml and 5-60 mug/ml respectively.

Chemoprotective effect of curcumin against cyclophosphamide toxicity.

Abstract: Life-span of mice treated with a chronic lethal dose of cyclophosphamide was considerably increased by the simultaneous administration of turmeric extract or curcumin (40.8 percent and 91.7 percent respectively ). Similarly leukoplnia produced by cyclophosphamide was reduced by these agents but lowered haemoglobin levels remained unaltered. Increased glutamate-pyruvate transaminase, alkaline phosphates and thiobarbituric acid- reacting material in the liver were also reduced by turmeric and curcumin.

1,3,4-oxidiazole thiones as inflammation inhibitors.

Abstract: 5-[2-Hydroxy phenyl]-1,3,4-oxadiazole-2 (3H) thione (II) was synthesized by cyclization of salicylic acid hydrazide (I). II was further converted to 5-[2-hydroxy phenyl ]-3-[arylaminomethyl)-1,3,4-oxadiazole 2(3H) thiones (III) and their hydrochlorides (IV). All the compounds were evaluated for their ability to inhibit carrageenin induced rat's paw oedema. IIIc and IIIe were found to have better activity than phenylbutazone with higher ALD 50 values.

Studies in aryloxylated benzotriazoles.

Abstract: Some new chlorosubstituted phenoxy acetyl and propionyl benotriazoles were synthesised and screened for their anti-inflammatory, analgesic, antibacterial and antifungal properties. Trichlorophenoxy acetyl benzotriazole exhibited better anti-inflammatory activity than its propionyl derivative. The 2,5-dichlorophenoxy acetyl bbenzotriazole showed moderately better analgesic activity among the series. All the compounds showed mild to moderate antibacterial and antifungal activity.

Synthesis of new 1, 3, 4-oxadiazolo [3, 2-a]-s-triazine-5, 7-dithiones and the - dithionone analogues as potential antifungal agents.

Abstract: N1-Aryl-N3-(5-aryl-1, 3, 4-oxadiazol-2-yl) thioureas (II a-d) on cyclocondensation with CS2/KOH and ethyl chloroformate afford 2, 6-diaryl-1, 3, 4-oxadiazolo [3, 2-a]-s-triazine-5, 7-dithiones (III a-d) and their - thionones analogues (IV a-d) respectively. 2, 6-Diaryl-5-thioxo-1, 3, 4-oxadiazolo [3, 2-a]-s-triazin-7-ones (VI a-d) are obtained by trating oxadiazolycarbamates (V a-b), the condensation products of 1, 3, 4-oxadiazoles and ethyl chloroformate, with aryl isothiocyanates. The compounds II to VI have been screened for their antifungal activity against A. flavus and F. solani.

Albumin microspheres and betacyclodextrin inclusion complex containing diclofenac sodium

Abstract: Diclofenac Sodium was formulated as microspheres using egg albumin by chemical cross linking and as inclusion complex with betacyclodextrin. Studies were done on entrapment efficiency,Particle size, diffusion and dissolution pattern.

Simultaneous estimation of penbutolol sulfate and hydrochlorothiazide from tablets by RP-HPLC.

Abstract: A reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of penbutolol sulfate and hydrochlorothiazide in tablets. A Chromegabond M C 18 column with a mobile phase of 50 mM Na2HPO4: acetonitrile (60:40 v/v) adjusted to pH 3.5 with orthophosphoric acid was used. The flow rate was 1 mL/min and effluent was monitored at 220 nm. Samples as low as 278 ng/ml of penbutolol sulfate and40 ng/ml of hydrochlorothiazide can be estimated. Propranolol HCI was used as internal standard, and the order of elution was hydrochlorothiazide (HTZ) : propranolol : penbutolol (PEN) (3.43 : 5.57 : 10.76 minutes,respectively).

Study of abietic acid-sorbitol derivatives as microencapsulating materials.

Abstract: Abietic acid was isolated from rosin N Grade. Abietic acid-sorbitol derivatives were prepared and evaluated for their physicochemical properties and as material for microencapsulation by pan coating technique, using salicylic acid as a model drug. The coated microcapsules were evaluated for moisture absorption, flow properties, friability and release characteristics. The result showed that the materials having lower acid values possedd moisture protection properties and also prolong the release of the drug.

Spectrophotometric determination of diclofenac sodium in pharmaceutical dosage forms.

Abstract: A simple colorimetric method is described for the determination of diclofenac sodium in pharmaceutical formulation based on the reaction of the drug in acidic media with sodium cobalti nitrite to form a light yellow colored complex ( lambda max 368 nm ) which obeys Beer's Law in 25 - 125 mug/ml concentration range.

High performance liquid chromatographic determination of nonoxynol-9 in contraceptive preparations.

Abstract: A high performance liquid chromatographic method for the determination of nonoxynol-9 in contraceptive preparations is described using Lichosorb RP-8 column, eluate being monitored at 280 nm using phenylbutazone as internal standard.

Cellulose acetate pathalate matrix in NaF tablets

Abstract: The formulation of NaF tablets using Cellulose Acetate Phthalate (CAP) as matrix material, with an intention to prepare slow release buccal tablets for the prevention of dental caries is developed. Various micrometric studies of granules and tablets (containing different concentrations of CAP are studied. Dissolution studies are also carried out in distilled water at 37 +/- 1 degree. Significant variations in granule and tablet) parameters depending upon the concentration of CAP is observed. The in-vitro drug release reveals that T80 percent values are above 90 min for formulations containing more than 30 percent CAP. The drug dissolution is diffusion controlled and follow the Higuchi equation. The fraction of the drug release is proportional to time 1/2. Preliminary clinical data will also be discussed.

Chemical constitution of drumstick polysaccharide.

Abstract: Spectrophotometric studies of the mucilage, designated as Drum stick polysaccharide (DSP), isolated from the pods of Moringa pterygosperma Gaertn, revealed the presence of galactose, dextrose, xylose and sodium, potassium, magnessium, calcium salts of glucuronic acid. Contrary to the definition of mucilages, the presense of dextrose was an exception in the present case.

Differential pulse polarographic determination of diazepam in pharmaceutical formulations.

Abstract: Differential pulse polarography has been employed for the determination of diazepam in pharmaceutical formulations. The well defined peaks used for analytical purposes were those produced by the 2e-, reduction of the azomethine group. Analytical procedure has been described for the determination of the drug. (Standard diviation : 1.2 percent Correlation coefficient : 0.968 and detection limit : 2.4 x 10(-7)M).

Phyllanthin and hypophyllanthin lack anticancer activity

Abstract: Phyllanthin and Hypophyllanthin are ligan constituents isolated from Phyllanthus niruri. When tested on various cancer cell lines these compounds did not show significant cytotoxic effect on any cell line.

Formulation and evaluation of despersible tablets of poorly soluble drugs.

Abstract: Dispersible tablets of three poorly soluble drugs namely sulphamethoxazole, piroxicam and oxyphenbutazone formulated with potato starch and MCC fulfilled all official (B.P.) requirements and gave fast and rapid dissolution of the medicament. Dissolution followed first-order kinetics.

Differential pulse polarographic determination of dantrolene.

Abstract: The differential pulse polarographic behaviour of dantpolene has been investigated over the pH range 2.0 to 12.0. The best defined peaks for the determination of the title compound are obtained in the buffer of 4 greater than pH less than 6. This technique has been applied to the determination of dantrolene in pharmaceutical formulations. Both standard addition and calibration methods are used. The lower detection limit is found to be 25 ngml(-1). The method is accurate and reproducible with 98.9 percent - 99.6 percent +/- 0.7.

Quantitative determination of bacosides by UV-spectrophotometry. Highly accessed article

Abstract: Spectrophotometric method for quantitative determination of total Bacosides as Bacoside-A (the active constituent) - a mental retention capacity enhancer is reported.