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JournalISSN: 1672-9609

Insect Science 

Wiley-Blackwell
About: Insect Science is an academic journal published by Wiley-Blackwell. The journal publishes majorly in the area(s): Biology & Population. It has an ISSN identifier of 1672-9609. Over the lifetime, 2122 publications have been published receiving 31966 citations.
Topics: Biology, Population, Medicine, Gene, Genus


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Journal ArticleDOI
TL;DR: A comprehensive but simple‐to‐use software package called DPS (Data Processing System) has been developed to execute a range of standard numerical analyses and operations used in experimental design, statistics and data mining in entomology.
Abstract: A comprehensive but simple-to-use software package called DPS (Data Processing System) has been developed to execute a range of standard numerical analyses and operations used in experimental design, statistics and data mining. This program runs on standard Windows computers. Many of the functions are specific to entomological and other biological research and are not found in standard statistical software. This paper presents applications of DPS to experimental design, statistical analysis and data mining in entomology.

892 citations

Journal ArticleDOI
TL;DR: This review summarizes current understanding of the cellular immune response of insects and identifies hemocytes as important sources of a number of humoral effector molecules required for killing different foreign invaders.
Abstract: The innate immune system of insects is divided into humoral defenses that include the production of soluble effector molecules and cellular defenses like phagocytosis and encapsulation that are mediated by hemocytes. This review summarizes current understanding of the cellular immune response. Insects produce several terminally differentiated types of hemocytes that are distinguished by morphology, molecular and antigenic markers, and function. The differentiated hemocytes that circulate in larval or nymphal stage insects arise from two sources: progenitor cells produced during embryogenesis and mesodermally derived hematopoietic organs. Regulation of hematopoiesis and hemocyte differentiation also involves several different signaling pathways. Phagocytosis and encapsulation require that hemocytes first recognize a given target as foreign followed by activation of downstream signaling and effector responses. A number of humoral and cellular receptors have been identified that recognize different microbes and multicellular parasites. In turn, activation of these receptors stimulates a number of signaling pathways that regulate different hemocyte functions. Recent studies also identify hemocytes as important sources of a number of humoral effector molecules required for killing different foreign invaders.

588 citations

Journal ArticleDOI
TL;DR: Extensive qualitative and quantitative high throughput analyses of temporal and spatial variations in gene expression, protein level and activity, and metabolite concentration will accelerate not only the understanding of the overall mechanisms of direct defense, but also accelerate the identification of specific targets for enhancement of plant resistance for agriculture.
Abstract: Plants respond to insect herbivory with responses broadly known as direct defenses, indirect defenses, and tolerance. Direct defenses include all plant traits that affect susceptibility of host plants by themselves. Overall categories of direct plant defenses against insect herbivores include limiting food supply, reducing nutrient value, reducing preference, disrupting physical structures, and inhibiting chemical pathways of the attacking insect. Major known defense chemicals include plant secondary metabolites, protein inhibitors of insect digestive enzymes, proteases, lectins, amino acid deaminases and oxidases. Multiple factors with additive or even synergistic impact are usually involved in defense against a specific insect species, and factors of major importance to one insect species may only be of secondary importance or not effective at all against another insect species. Extensive qualitative and quantitative high throughput analyses of temporal and spatial variations in gene expression, protein level and activity, and metabolite concentration will accelerate not only the understanding of the overall mechanisms of direct defense, but also accelerate the identification of specific targets for enhancement of plant resistance for agriculture.

384 citations

Journal ArticleDOI
TL;DR: The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.
Abstract: RNA interference (RNAi) refers to the process of exogenous double-stranded RNA (dsRNA) silencing the complementary endogenous messenger RNA. RNAi has been widely used in entomological research for functional genomics in a variety of insects and its potential for RNAi-based pest control has been increasingly emphasized mainly because of its high specificity. This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research. In this review, we also briefly introduce the two possible dsRNA uptake machineries, other dsRNA delivery methods and the history of RNAi in entomology. Factors that influence the specificity and efficiency of RNAi such as transfection reagents, selection of dsRNA region, length, and stability of dsRNA in RNAi research are discussed for further studies.

294 citations

Journal ArticleDOI
TL;DR: Different interpretations of the relationship between the net reproductive rate and the intrinsic rate of increase are discussed to clarify possible misunderstanding in the literature.
Abstract: Age-stage, two-sex life tables of the melon fly, Bactrocera cucurbitae (Coquil- lett) (Diptera: Tephritidae), reared on cucumber (Cucumis sativus L.), sponge gourd (Luffa cylindrica Roem) and a carrot medium (mashed Daucus carota L. mixed with sucrose and yeast hydrolysate) were constructed under laboratory conditions at 25 ± 1 ◦ C, 65% ± 0.5% relative humidity, and a photoperiod 12 : 12h (L : D). The intrinsic rates of increase of B. cucurbitae were 0.1446, 0.1412 and 0.0688 days on cucumber, sponge gourd, and car- rotmedium,respectively.Thehighestnetreproductionratewas172offspringperflyreared on sponge gourd. The mean generation times of B. cucurbitae ranged from 34 days reared on cucumber to 56 days reared on carrot medium. The life history raw data was analyzed using the traditional female age-specific life table and compared to results obtained using the age-stage, two-sex life table. When the age-specific female life table is applied to an age-stage-structured two-sex population, survival and fecundity curves will be improperly manipulated due to an inability to include variation in preadult development time. We discussed different interpretations of the relationship between the net reproductive rate and the intrinsic rate of increase to clarify possible misunderstanding in the literature.

290 citations

Performance
Metrics
No. of papers from the Journal in previous years
YearPapers
202375
2022160
2021199
2020125
201992
2018101