Showing papers in "Journal of Dalian Medical University in 2015"

Effects of resveratrol on female reproductive system malignant tumors

Abstract: Resveratrol has therapeutic effects on female reproductive system tumors. The mechanism of its function is very complex including inhibition of tumor cell proliferation and induction of tumor cell differentiate. Resveratrol can also hinder tumor cell invasion and prevent distant metastases. Female reproductive system tumors seriously threat women's health and quality of life and analysis of effective therapeutic drugs is of great significance. This review summarizes recent research progress of resveratrol on female reproductive system malignant tumors.

Efficacy and safety of EGFR-TKIs as first-line treatment in 112 elder patients with advanced non-small cell lung cancer

Abstract: Objective To study the efficacy and safety of EGFR- TKIs as the first- line therapy in elder patients with advanced non- small cell lung cancer. Methods 112 cases of advanced non- small cell lung cancer in patients aged beyond70 years were analyzed,including 68 treated with Gefitinib( 250 mg,qd),15 with Erlotinib( 150 mg,qd),and 29 with Icotinib( 125 mg,tid). To study the efficacy and median progression- free survival of EGFR- TKIs therapy. Results Of the 112 patients who received EGFR- TKI treatment,109 patients finished the course. No CR was observed. PR,SD and PD were found in 26,42 and 41 patients,respectively with ORR 23. 8% and DCR 62. 4%. 3 patients discontinued the medication because of intolerance. m PFS was 6. 0 months. Conclusions EGFR- TKIs is a safe and effective targeted therapy in old patients with advanced non- small cell lung cancer. No difference in efficacy can be concluded in the comparison among Gefitinib,Icotinib and Erlotinib.

Risk factors for MDR- TB and XDR- TB in Dalian patients

Abstract: Objective To explore the risk factors for MDR- TB and XDR- TB in Dalian patients. Methods We retrospectively analyzed the clinical records of 954 TB Patients with sputum and culture- proven MDR- or XDR- TB,who were registered from January 1,2012 to January 1,2014 in the Dalian Tuberculosis Hospital. Results All TB patients were culture-positive for M. tuberculosis complex and had positive sputum- smear microscopy results. Among first- time patients,4. 69%were MDR- TB and 0. 88% were XDR- TB. In recurrent patients,MDR- TB and XDR- TB were 26. 57% and 6. 27 %,respectively. Significant risk factors for MDR / XDR- TB included incomplete treatment regimen,inadequate doses,previous use of a fluoroquinolone and injectable agent,poor compliance,and diabetes. Conclusion Inadequate initial regimen and diabetes are important risks of drug resistance. The mechanisms of TB drug- resistance are complex and need further studies.

Research progress in the treatment of non- small cell lung cancer with malignant pleural effusion by endostar combined with cisplatin infusion

Abstract: Malignant pleural effusion( MPE) is a common complication of advanced non- small cell lung cancer( NSCLC) and has seriously impacts on patient's quality of life. Recombinant human endostatin injection( Endostar),SFDA approved since 2006,combined with cisplatin intrapleural perfusion showed good effectivity and good safety for the treatment of NSCLC patients with MPE. The recent clinical research of Endostar combined with cisplatin intrapleural perfusion for the treatment of NSCLC MPE are reviewed.

Comparison of clinical effects between unilateral and bilateral percutaneous vertebroplasty for osteoporotic vertebral compression fractures

Abstract: Objective To compare the clinical efficacy of unilateral versus bilateral percutaneous vertebroplasty( PVP) in the treatment of osteoporotic vertebral fractures( OVCFs). Methods A retrospective study was carried out on 65 patients with osteoporotic vertebral compression fractures from April 2007 to February 2014 at the department of orthopaedics of the Xinhua Hospital affiliated of Dalian University. Patients were assigned to two groups and received unilateral( 36 cases) or bilateral( 29 cases) PVP treatment under local anesthesia. The operative time,amount of bone cement injected,frequency of X- ray exposure and postoperative VAS score were compared. Results The unilateral group had an average operative time of( 25 ± 6) min,which was significantly shorter than that of bilateral group( 45 ± 5) min( P = 0. 011). The leakage rate of bone cement was 8. 3%( 3 /36) in the unilateral group and 6. 9%( 2 /29) in the bilateral group. There was no significant difference between the two groups( P 0. 05). The X- ray exposure frequency in the unilateral group was( 10. 5 ± 2. 5)times,which was significantly less than that in the bilateral group( 19. 4 ± 3. 0) times( P 0. 001). No statistical difference could be found in VAS assessment 24 h,6 months,and 12 months after the surgery between the two groups( P 0. 05). Conclusion Unilateral PVP can be used as preferred method for the treatment of OVCFs due to the advantages of shorter operation time,less X- ray exposure frequency and low cost.

Prognostic value of susceptibility- weighted imaging in massive cerebral infarction

Abstract: Objective To investigate the susceptibility- weighted imaging( SWI) characters in the massive cerebral infarction and evaluate the role of SWI in prognostic assessment of the large ischemia stroke. Methods 43 patients with massive cerebral infarction,defined by the Adamas rules as the infarction diameters were bigger than 3 cm2,and related to more than two main vascular territory,were performed SWI within 7 days,2 weeks and 3 months. Clinical function scores were acquired with the NIHSS. Characteristics of routine MRI and SWI images were analyzed. Presence of hemorrhage and correlated arterial thrombosis and collateral circulation after massive cerebral infarction were studied. Correlation of imaging and NIHSS score changes was analyzed. Results Hemorrhage was revealed by SWI in 34 out of 43( 79%) massive cerebral infarction. There was a negative correlation between the extent of hemorrhage and the change of NIHSS scores( P 0. 01).Arterial thrombosis was found in 30 cases( 70%). The proximal location of the thrombosis to the cerebral necrosis and the thrombus length were negatively correlated to the change of NIHSS scores( P 0. 01). Collateral circulation was seen in 31cases( 72%). There was a positive correlation between the extent of collateral circulation displayed in the SWI and the change of NIHSS scores( P 0. 01). Conclusion SWI can show the hemorrhage,correlated arterial thrombosis and the collateral circulation of massive ischemic stroke and can be used to measure the changes,thus is helpful in guiding the clinical prognosis.

Detection of serum leptin and soluble leptin receptor levels and its significance in patients with systemic lupus erythematosus

Abstract: Objective To investigate the serum levels of leptin and soluble leptin receptor in patients with systemic lupus erythematosus( SLE) and to analyze their correlation with SLE disease activity and clinical parameters Methods Serum leptin and soluble leptin receptor levels of 87 patients with SLE and 85 matched healthy controls were measured by ELISA assays The liner correlation analysis was used to evaluate the relationship between levels of leptin and soluble leptin receptor with their clinical parameters Results Compared to normal controls,serum level of leptin was significant higher in SLE patients [( 7 06 ± 3 19) ng / m L vs( 5 36 ± 2 66) ng / m L,P 0 05] No significant difference was found in soluble leptin receptor levels between SLE patients and controls Furthermore there were no differences in the levels of leptin and soluble leptin receptor between active and inactive SLE patients,patients with and without lupus nephritis( LN) and patient with and without arthritis,respectively A positive association was observed between leptin levels in SLE patients and body mass index( BMI)( r = 0 375,P = 0 022); a negative correlation was observed between leptin and soluble leptin receptor levels in SLE patients( r =- 0 219,P = 0 041) The serum soluble leptin receptor levels were positively correlated with ESR,Ig G,Ig A and Ig M( r = 0 387,P = 0 001; r = 0 336,P = 0 009; r = 0 331,P = 0 010; r = 0 366,P =0 004,respectively) Conclusion Abnormal levels of leptin might be involved in the pathogenesis of SLE However,levels of serum leptin and soluble leptin receptor could not be used as a clinical index to evaluate the severity of SLE

Infection- related risk factor analysis after pancreaticoduodenectomy

Abstract: Objective To investigate infection- related risk factors after pancreaticoduodenectomy( PD). Methods Totally 118 patients,who received PD from 2009 January to 2014 June in our hospital,were retrospectively analyzed. 21 patients developed infection after surgery and were included in infection group; the remainder non- infection patients were classified as control group( 97 cases). General information,preoperative,intraoperative and postoperative factors of patients in two groups were analyzed by single factor analysis and multivariate Logistic regression analysis. Results Bacteria were the main source of infection after PD including mainly Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumoniae,and Staphylococcus aureus. Single factor analysis showed that postoperative pancreatic fistula,postoperative albumin,operation time,preoperative bilirubin,postoperative hemoglobin,peri- operation blood transfusion and fasting blood glucose after operation were significantly different( P 0. 05). Multivariate Logistic regression analysis showed that pancreatic leakage after operation,postoperative hypoproteinemia,operation time,postoperative hyperglycemia were independent risk factors of infection after PD( P 0. 05). Conclusion It should be reducing the operation time and preventing pancreatic fistula,hypoproteinemia and hyperglycemia after operation to reduce the incidence of postoperative infection of PD.

Clinical effect of microecologics as an adjuvant therapy on infants' eczema

Abstract: Objective To study the clinical effect of microecologics on infants' eczema. Methods Totally 180 eczema patients were randomly divided into two groups: the control group and the experimental group with 90 patients in each group.Patients in the control group were treated with routine symptomatic treatment and patients in the experimental group were treated with routine symptomatic treatment plus combination of four living bacterium tablet. The microecologics were taken orally,one tablet a time and twice a day for one month. The effective rate of the two therapeutic methods was analyzed and the levels of IL- 4,IL- 10,Ig E,IFN- γ and Th1 / Th2 ratio in blood were examined. The relapse rate of the two groups in the three months follow up visit was analyzed. Results Effective rate in the experimental group was 91. 1%,much higher than the control group with statistical significance( P 0. 05). Levels of IL- 4,IL- 10 and Ig E in blood serum decreased significantly in the experimental group when compared to the control group( P 0. 05) and level of IFN- γ and Th1 / Th2 ratio increased significantly in the experimental group when compared to the control( P 0. 05). The 3 months eczema relapse rate was 26. 7% in the experimental group and 68. 9% in the control group. The difference between the two groups was statistically significant. Conclusion Applying microecologics as an adjuvant therapy for eczema treatment could increase the effective rate and decrease the 3 months relapse rate,meanwhile enhance the self- stabilization of immune system. Microecologics has very wide popularization and application prospects for eczema treatment.

Research progress of the Bcl- 2 gene family

Abstract: Bcl- 2 family proteins are key regulatory molecules of apoptosis. Mitochondria and endoplasmic reticulum etc.play important roles in the process of apoptosis. Bcl- 2 family proteins can have direct or indirect effects on mitochondria to play its function. Bcl- 2 gene family has also a unique role in ischemia- reperfusion injury( IRI). The expression and regulation of Bcl- 2 family has great influence on tumor cell apoptosis. How to regulate the expression of Bcl- 2 family members and the expression- related target therapy have become a direction of future research. This article summarizes the present research progress.

Association of microsatellite polymorphism of MICA gene with susceptibility to ovarian cancer

Abstract: Objective To explore the relationship between microsatellite polymorphism of MICA gene exon 5 and the risk of ovarian cancer. Methods A total of 95 ovarian cancer patients and 99 unrelated healthy people were recruited in this study. PCR-capillary electrophoresis method was used to detect the microsatellite genotypes of MICA gene exon 5. Results Five alleles were observed in both groups,A4( 179 bp),A5( 182 bp),A5. 1( 183 bp),A6( 185 bp) and A9( 194bp),respectively. In the control group,A5. 1 was the most common allele( 33. 8%). The frequency of A5 allele of MICA gene in ovarian cancer patients was significantly higher than that in controls( 31. 1% vs. 19. 2%,P 0. 01). Conclusion A5 allele of MICA gene exon 5 could be associated with high risk of ovarian cancer.

Distribution of Hepatitis C virus genotype in Anshan Central Hospital

Abstract: Objective To investigate the distribution of HCV genotype in Anshan. Methods HCV genotype was determined by RT-nested-PCR in 189 cases of anti-HCV and HCV RNA positive patients in Anshan. Results In the serum of the189 anti-HCV and HCV RNA positive patients,1b and 2a were detected. No mixed genotype was found. 11 cases were negative. Conclusion There are only two HCV genotypes,1b and 2a,found in Anshan. They have almost equal distribution with 53% 1b and 47% 2a,respectively.

Comparison of miniscrews and headgear facebow in treatment of skeletal class II adults of hyperdivergent

Abstract: Objective To compare miniscrew and headgear facebow in skeletal class Ⅱ of hyperdivergent adults and to evaluate the clinical efficacy. Method Thirty- two patients,who were treated with miniscrew and headgear facebow higher traction for skeletal class Ⅱ,were divided into two groups. Cephalometric analysis was performed to observe the changes of the characteristic lands on the dentofacial morphology before and after treatment. Results The course of miniscrew was shorter than that of headgear facebow( t =- 3. 89,P 0. 01). The decreases of B- X distance,U1- X,U1- Y,U6- X,UL-EP,ANB,UL- EP and LL- EP with miniscrew were much more than those of headgear facebow higher traction( P 0. 01). The increase was much more in B- Y distance( P 0. 01). The increase of U6- Y was less in headgear facebow( P 0. 05). The differences of A- X and A- Y were not statistically significant( P 0. 05). Conclusion Miniscrew are much better in En- masse retraction of anterior teeth and controlling the molars and can promote the mandibular with counterclockwise rotation. It has obvious advantages in improving facial profile of class Ⅱ.

Clinical observation on the curative effect of knee osteoarthritis with mnimally invasive arthroscopic operation and local injection

Abstract: Objective To observe the curative effect of knee osteoarthritis with mnimally invasive arthroscopic operation and local injection. Methods Choose 90 patients with knee osteoarthritis diagnosed as hospitalized patients from March2012 to February 2013. They were randomly divided into Dan- wei treatment group,Qu- li treatment group,and the control group. There were 30 cases in each group. Patients were informed consent and signed informed consent. All patients do Arthroscopic inflammation of the synovial membrane. Dan- wei treatment groups was with local pain points for injection of compound Dan red injection( 2 m L) and vitamin B12 injection( 0. 5 mg). Qu- li treatment group was with Umcy Ann Ned injection( 2 m L) with lidocaine hydrochloride injection( 1 m L). 1 time a week,3 times were a course of treatment.The control group was only with arthroscopic minimally invasive surgery. Follow up for 6 months after treatment. The clinical effect of Lysholm was evaluated by Lequsne efficacy and knee function score. Results Two scoring results are displayed.Compared with control group,The clinical therapetic effect of Dan- wei treatment group and Qu- li treatment group were obvious( P 0. 05). Compared the score of patients in Dan- wei treatment group and Qu- li treatment group,the difference of statistics are not obviously( P 0. 05). Conclusion There is good clinical therapetic effect of minimally invasive surgery combined with compound Dan red injection and vitamin B12 local acupoint injection for osteoarthritis of knee joint,and avoided the complications caused by the Qu- li treatment group.

Effect of insulin on InsR expression in rats with diabetic cognitive impairment

Abstract: Objective To observe the change of congnitive function and the expression level of hippocampal insulin receptors( InsR) in 1 month and 3 months of diabetic rats,explore the effect of InsR on diabetic rats and investigate that whether insulin can be applied to prevent diabetic cognitive impairment. Methods 36 healthy adult male SD( Sprague- Dawley)rats,weight 180- 240 g,were divided into 3 groups randomly: diabetic group( n = 12),insulin treatment group( n = 12)and control group( n = 12),and then each group was divided into 2 groups equal in amount randomly: 1 month group and3 months group. Diabetic group and insulin treatment group rats were induced by streptozotocin injection. Fixed time to measure blood glucose of all rats twice a week. Insulin treatment group rats were injected insulin daily,and we adjusted insulin dosage according to the blood glucose level to ensure blood glucose in normal range. All the rats underwent the test of Morris water maze in 1 month and 3 months. After the assessment on cognitive function of the 1 month and 3 months rats that experienced the Morris water maze experiment,the expression of InsR in brain were observed. Results Compared with the control group,1 month group of diabetic rats appeared cognitive impairment and their expression of InsR reduced,which has statistical difference( P 0. 05). Compared with 1 month diabetic group,the cognitive impairment of 3 months diabetic group was obviously aggravated and their expression of InsR reduced more significantly,which has statistical difference as well( P 0. 05). 1 month group of insulin treatment rats with no significant cognitive impairment,and their expression of InsR reduced slightly. Compared with the control group,there are no difference. Compared with the 1 month diabetic group,which has statistical difference( P 0. 05). 3 months group of insulin treatment rats appeared cognitive impairment and their expression of InsR reduced obviously. Compared with control group,which has statistical difference( P 0. 05). Compared with 1 month group of insulin treatment rats,which has statistical difference as well( P 0. 05).Compared with 3 months group of diabetic rats,there are no statistical difference. Conclusion Diabetic cognitive impairment is closely related to InsR,the reduced InsR expression may contribute to diabetic cognitive impairment. Early intervention of insulin has protective effect on the brain,then delay cognitive impairment. However,as the extension of the duration,the insulin treatment group rats still show cognitive impairment,which suggests that long- term application of insulin can not prevent the occurrence of cognitive impairment. Mechanisms need to be further explored.