Showing papers in "Journal of the Indonesian Tropical Animal Agriculture in 2019"
TL;DR: The results showed that the age of Bali bull could be used as an indicator to determine its BW and semen qualities, and the age correlated significantly with BW, SV, IM, SC.
Abstract: The aim of this research was to analyze the correlation of age of Bali bull to the body weight and semen qualities. The materials were 50 Bali bulls data collected from 2012 to 2018 . The recorded data were consisted of semen production s (n = 1304) and body weight (n = 1544). The observation was conducted to analysis the interrelationship between the Bali bulls age and body weight (BW), to the semen volume (SV), individual motility (IM) and sperm concentration (SC). The obtained d ata was analyzed with One-Way ANOVA analysis by using SPSS 24 and followed with Duncan’s Multiple Range Test to determine any significant differences. The correlation between Bali bulls age (the independent variable) and semen quality (the dependent variable) was computed by using Pearson correlation method and presented in linear regression models. The results showed that the Bali bulls age significantly affect ( P <0.0 5 ) the BW from the age of 1 to 10 years old. The age also showed a significant effect ( P <0.0 5 ) on the semen qualities. The age correlated significantly ( P <0.0 5 ) with BW, SV, IM, SC were 0.658 , 0.3 86, 0.1 34 and 0. 086, respectively. In conclu sion, the age of Bali bull could be used as an indicator to determine its BW and semen qualities .
14 citations
TL;DR: In this paper, the authors aimed to analyze factors and models of business sustainability of smallholder layer farms in Kendal Regency, Indonesia, and found that social, economic, institutional and income variables had a significant and positive influence on business sustainability.
Abstract: This study was aimed to analyze factors and models of business sustainability of smallholder layer farms in Kendal Regency, Indonesia. Purposive sampling method was used to select 120 active layer chicken farmers in Sukorejo, Pageruyung, Patean, Limbangan, and Boja Districts as research respondents with the ownership of 300 to 10,000 birds. Research variables consisted of three external variables (social, economic, and institutional) and two internal variables (income and business sustainability). Closed questionnaires were used to collect data. The data were analyzed descriptively using Structural Equation Modeling (SEM) by AMOS 21 software. The results showed that the exogenous and endogenous variables met the modeling criteria with Chi square value = 160.764; probability = 0.380; CMIN / DF = 1.031; GFI = 0.892; AGFI = 0.841; TLI = 0.998; CFI = 0.998; RMSEA = 0.016. Social, economic, institutional and income variables had a significant and positive influence (P≤0.05) on business sustainability. The constructed model had a strong and positive relationship, so it could illustrate the sustainability model of layer business in Kendal Regency.
9 citations
TL;DR: In this article, a study had been conducted to evaluate the performance of Islamic Boarding Schools (IBS) in developing the beef cattle agribusiness partnerships network in Central Java.
Abstract: A study had been conducted to evaluate the performance of Islamic Boarding Schools (IBS) in developing the beef cattle agribusiness partnerships network in Central Java. This study involved 24 IBS with each IBS selected two respondents, namely one from internal ( santri ) and one from external (farmer community). Sample determination uses purposive sampling method. This study uses Gap Analysis by determining the Customer Satisfaction Index (CSI) value. Primary data were collected through direct observation and interviews with respondents. Secondary data was obtained from relevant government institutions. The results showed that the beef cattle agribusiness partnership network that was developed by IBS still did not satisfy for santries and farmer communities ( CSI. s.total : 30.73% and CSI. f.total : 47.05%). It was concluded that farmer communities were very enthusi astic about establishing the beef cattle agribusiness partnerships with IBS; the santries needed additional livestock curriculums; IBS needed training and assistance from the other parties (government, private sector, etc.) in order to improve the ability to raise beef cattle and the ability to train, instruct and plan the beef cattle farming development in surrounding area. Increasing the ability of IBS automatically can increase the effectiveness of IBS role as an empowerment institution for surrounding community.
8 citations
TL;DR: In this paper, a study was conducted to identify and to control the behavior of cattle farmers towards biosecurity in Lamasi District - Luwu Region, South Sulawesi Province.
Abstract: Beef cattle are meat producers which are beneficial to humans. The purpose of this study was to identify and to control the behavior of beef cattle farmers towards biosecurity in Lamasi District - Luwu Region, South Sulawesi Province. This type of research was descriptive research. The research method was survey to participatory research and the Focus Group Discussion. Total sample was 50 respondents. Data were obtained through interview using questionnaires and observations. Data were analyzed using descriptive statistic. Likert scale 1 until 3 were used to know the response level of disagree and agree about biosecurity. The results showed that beef cattle farmers less agree to biosecurity adoption. Based on subjective norm, farmers’ behaviors agree to adopt biosecurity if they were supported by community leaders and other livestock farmers or farmer groups. Behavior control which becomes the main obstacles to implementing biosecurity are habits, prior implementation and risks. T he supporting factors needed time, can be tested on a small scale cattle business, according to the needs of cattle farmers, needed workers, needed knowledge, skills, and information about biosecurity . Beef cattle farmers should be motivated to adopt biosecurity in order to get a healthy beef cattle.
7 citations
TL;DR: In this article, the authors evaluated the impact of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chicks, and concluded that dietary supplements may prevent the pale, soft and exudative (PSE)-like condition in broiler meats.
Abstract: The objective of the present study was to evaluate the impact of dietary supplementation of formic acid, butyric acid or their combination on carcass and meat characteristics of broiler chicks. A number of 288 day-old-chicks (Lohmann MB-202 meat broilers) were distributed to four groups, including CONT (basal diet with no additive as a control), FORM (basal diet containing 0.1% formic acid), BUTR (basal diet with 0.03% butyric acid) and FORM+BUTR (basal diet with 0.1% formic and 0.03% butyric acid). At day 35 the birds were slaughtered and eviscerated, and from which the breast meat was obtained. Carcass and cut yields of broilers were not different (P>0.05) among CONT, FORM and BUTR birds. The combination of formic and butyric acids increased (P<0.05) the relative weight of back of broilers. Meat pH value was higher (P<0.05) in organic acids treated than in non-treated birds. Organic acids lowered (P<0.05) drip loss of broiler meat. Butyric acid increased (P<0.05) moisture and decreased (P<0.05) the content of meat protein. Formic acid increased (P<0.05), whereas butyric acid and combination of formic and butyric acids decreased (P<0.05) lightness values o f breast meat. Yellowness values of meat increased (P<0.05) with organic acid administration. Intense bands of 25 kDa (phosphoglycerate mutase) was observed in most of meat samples from the treated birds, but not in meat from the control birds. In conclusion, dietary supplementation of organic acids was capable of improving the meat quality of broiler meats. The treatments may prevent the pale, soft and exudative (PSE)-like condition in broiler meats.
7 citations
TL;DR: The SNP g.1133C>G of the MC4R gene may be used as a marker-assisted selection for SH trait in Madura cattle.
Abstract: Melanocortin-4 receptor (MC4R) gene has an important role in the regulation of feed intake and energy balance control. The objective of this study was to identify the single nucleotide polymorphisms (SNPs) of MC4R gene and their association with growth traits in Madura cattle. A total of 198 calves were used in this study. Forward primer: 5’-GTCGGGCGTCTTGTTCATC-3’and reverse primer: 5’-GCTTGTGTTTAGCATCGCGT-3’ were used to amplify approximately 493 bp of MC4R gene. The results showed that two SNPs, g.1133C>G and g.1108C>T were identified by direct sequencing. The PCR-RFLP method was performed to genotype all individuals studied based on SNP g.1133C>G, and its SNP was significantly associated with shoulder height (SH) at yearling age (P G changed amino acid from valine to leucine. In conclusion, the SNP g.1133C>G of the MC4R gene may be used as a marker-assisted selection for SH trait in Madura cattle.
7 citations
TL;DR: Results indicated that self-formulated feed with inclusion of GTL at 4 ml (A1B4) improve body resistance and increase production performance of broilers.
Abstract: This study was conducted to evaluate the combination of ginger and turmeric extracts added with Lactobacillus spp. (GTL) on body resistance and productivity of broilers. 576 broiler s with an initial body weight of 36 ± 0.99 g were used and were observed for 35 days. GTL containing ginger extract (0.2 g/liter), turmeric extract (0.4 g/liter) and Lactobacillus spp. (2,997 x 107 cfu/ml), and was given since 1-day old . Parameters observed were bursa fabriscius, spleen, and thymus weights, gluthationine peroxidase (GSH-Px), superoxide dismutase (SOD), Lactobacillus spp., Coliform , and Eschericia coli , feed consumption, body weight gain, and feed conversion ratio (FCR). A completely randomized design (CRD) was assigned with 3x4 factorial pattern (3 types of feed, 4 levels of GTL), with 4 replications (12 birds each). First factor, types of feed (A) : self-formulated feed (A1), combination of self-formulated and commercial feed with 50 portion each (A2) and commercial feed (A3). Second factor, levels of GTL (B): 0 ml (B0), 2 ml (B2), 4 ml (B4) and 6 ml (B6) per litr e drinking water. Data were subjected to ANOVA and continued to Duncan test at 5% probability. Results indicated that self-formulated feed with inclusion of GTL at 4 ml (A1B4) improve body resistance and increase production performance of broilers.
7 citations
TL;DR: In this paper, the socioeconomic benefits of Jabres cattle as potential local livestock in two agro ecological zones, lowlands and middle zone in Bantarkawung Sub-district, Brebes, Central Java, were explored.
Abstract: This study aimed to explore the socio-economic benefits of Jabres cattle as potential local livestock in two agro ecological zones, lowlands and middle zone in Bantarkawung Sub-District, Brebes, Central Java. The data were collected by Participatory Rural Appraisal (PRA) method, which is an approach to families or individuals to analyze the reality of their living conditions. This study involved 60 farmers which selected by purposive sampling based on three considerations. The analytical model used to test differences is the analysis of independent T-test. The result showed that the farmers in the middle zone are younger and most of their cattle keeping motivation are for livelihood. Meanwhile, the farmers in the low lands are older and most of their cattle keeping motivation is for saving only (2.90±0.31). This condition caused the socio-economic benefit difference between those locations where the value of net production (185±27 kg/y), added value (IDR 4.900.000±1.204.000), and total benefits (IDR 9.289.000±1.542.000) were higher in the medium land than in the lowlands. Based on these reasons, it can be concluded that middle zone farmers have more possibility to sustain their farm than those of lowlands farmers.
7 citations
TL;DR: It was concluded that the polymorphism of bPit-1/HinfI in Pasundan cattle included of low category and was not associated with body weight.
Abstract: Bovine Pituitary specific transcription factor 1 (bPit-1) is one of amino acid that controling pituitary gland in mammals. The pituitary gland is important for secretion of growth hormone from growth genes. This study was carried out to detect polymorphism in the exon 6 of bPit-1 (g.1256G>A) in Pasundan cattle using PCR-RFLP method and its association with body weight. Total of 69 heads (15 males and 54 females) of Pasundan cattle from breeding station (BPPIBT-SP Ciamis, West Java) were used in this study. Research showed that two genotypes of bPit-1/ Hinf I gene were identified in this study i.e GG (0.90) and AG (0.10) with allele frequencies of 0.05 (A) and 0.95 (G). The polymorphic informative content (PIC) and number of effective allele ( n e ) values were 0.09 (low) and 1.11. respectively. The Chi-square ( χ 2 ) value in the population studied was 0.20 and in Hardy-Weinberg equilibrium ( χ 2 <5.99). It was concluded that the polymorphism of bPit-1/ Hinf I in Pasundan cattle included of low category and was not associated with body weight.
6 citations
TL;DR: It could be concluded that variant SNP in the LEPR may contribute to fatty acid composition without influencing odour and flavour traits in sheep.
Abstract: The aim of this study was to investigate the association and expression of LEPR with fat quality, flavour and odour in sheep. A total of 47 rams were used in this study. Fatty acids composition, BCFA (Branch Chanin Fatty Acids; MNA, MP, MOA, and EOA) and skatole (MI) representing fat quality, flavour and odour in sheep were measured at ages of 10-12 months old. Identification of novel variant and expression analysis of LEPR gene with different fat quality, odour and flavour were performed by PCR RFLP and qRT-PCR, respectively. A SNP in genomic region g.40854778 A>C of the LEPR gene was associated (P<0.05) with fat quality of saturated fatty acids (SFA) including [tricosanoic acid (C23:0) and tetracosanoic acid (C24:0)] and poly unsaturated fatty acid (PUFA) [docosahexaenoic acid (C22:6n3)]. However, the novel variant of LEPR was not significantly associated (P<0.05) with fat content, odour and flavour (MNA, MP, MOA, EOA, and MI). The mRNA expression analysis showed that LEPR mRNA expression was higher (P<0.01) in sheep found at the AA genotype. It could be concluded that variant SNP in the LEPR may contribute to fatty acid composition without influencing odour and flavour traits in sheep.
6 citations
TL;DR: In this article, the authors compared and determined the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Tris-clarified egg yolk), non-animal based extenders (Bioxcell®, lecithin based) and Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4 hours).
Abstract: The objectives of the present study were to compare and determine the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Tris-clarified egg yolk (Tris-cEY)), and non-animal based extenders (Bioxcell® (lecithin based) and Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4hours). Thirty six ejaculates were collected from six Balinese bulls and frozen in three extenders (Tris-cEY, Bioxcell® and Optixcell®) after equilibration in three different times (30 minutes, 2hours and 4hours). Computer-assisted sperm analysis (CASA), hypo-osmotic swelling test (HOST) and eosin nigrosin staining were used in the post-thawed semen analysis. There was a significant interaction between equilibration time and extender type for sperm motility, viability and membrane integrity. Thirty minutes equilibration time had the lowest values (P 0.05). Moreover, post-thawed semen which were extended in Optixcell® for 2 hours equilibration showed a better motility compared with the other extenders (P<0.05). In conclusion, two hours equilibration of semen with Optixcell® is sufficient for semen freezing. Four hours equilibration has the best sperm survival, independent of the extender type.
TL;DR: Designed primers from mitochondrial 12S rRNA gene could be used to identify dog and rat in raw beef containing these species meat by using multiplex PCR assay.
Abstract: The 12S rRNA gene is one of unique regions in mitochondrial genome usually used for phylogenetic studies and species identification. The objective of present study was to develop species specific primer s from mitochondrial 12S rRNA gene for identification of dog and rat in beef by using multiplex PCR assay . Three primer pairs of mitochondrial 12S rRNA gene specific for bovine, dog and rat were designed and selected to evaluate their specificity and fidelity. Moreover , a total of twelve DNA samples extracted from meat tissue were also prepared to test those primers using simplex and multiplex PCR. The PCR products were then visualized using 2% of agarose gel under the UV light and three of them were sequenced. In addition, sequence data were analyzed using Clustal Omega software and BLAST. The result showed that simplex PCR assay successfully amplified DNA targets which are respectively indicated by 155 bp (bovine), 244 bp (dog), and 491 bp (rat) of DNA bands. Furthermore, DNA sample sequences were identically similar to reference sequence used in this study. Multiplex and simplex PCR analyses also indicated that these primer pairs specifically amplified DNA target for each species in the samples containing various species. The results suggested that designed primers in this study could be used to identify dog and rat in raw beef containing these species meat. Further experiment should be conducted using meat-processed products and commercial meat products as samples.
TL;DR: In this article, the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphism s on fatty acid traits and cholesterol in lamb s were explored.
Abstract: This study was aimed to explore the effects of Betaine-Homocysteine Methyltransferase (BHMT) gene polymorphism s on fatty acid traits and cholesterol in lamb s . This study used a total of 147 blood samples for genotyping including 19 Javanese Fat-Tailed (JFT) , 16 Javanese Thin-Tailed (JTT) , 41 Composite Garut (CG) , 35 Compass Agrinak (CA) and 36 Barbados Black Belly Cross (BC) . A total of 61 rams as representative from five breed of sheep were selected for association study . Identification of BHMT single nucleotide polymorphisms was analyzed by PCR-RFLP method . Association of BHMT genotypes with fatty acid traits and cholesterol was performed by T-TEST. BHMT gen otyping resulted into three genotypes (CC, CT and TT). Gen e frequency of BHMT (g. 9947372 C>T) was in Hardy-Weinberg E quilibrium, excluding Javanese Fat-Tailed sheep. Association of BHMT genotypes with fatty acid traits resulted into a significant association (P T) of BHMT gene might be a useful marker for selecting and producing sheep meat with desirable fatty acids.
TL;DR: It was concluded that combined effect of FR and feed additives could significantly alleviate heat stress effects for broilers by improving growth performance and decreasing mortality rate.
Abstract: The objective of the current study was to evaluate the impacts of feed restriction (FR) for 3 hours and/or dietary supplementation with vitamins and minerals on growth, mortality rate, some carcass traits, and serum biochemistry indices of broiler chicks under heat stress in the open housing system. A total of 240 one-week old broilers were weighed and randomly assigned into 4 equal experimental treatments. Treatment 1 (T1) was fed ad libitum on basal diets, T2 birds were fed on basal diets with FR, T3 birds were fed ad libitum on basal diets with vitamin C (200 mg) + vitamin E (200 mg) + Cr (1.5 mg ) + Zn (100 mg) per kg diet, and T4 birds were fed on basal diets with FR and combined supplementation of vitamins and minerals. It was found that growth parameters improved in T2, T3, and T4, while the mortality rate was significantly reduced compared with control. FR and feed additives (T4) revealed marked increases in serum total protein and a decrease in total serum cholesterol. It was concluded that combined effect of FR and feed additives could significantly alleviate heat stress effects for broilers by improving growth performance and decreasing mortality rate.
TL;DR: In this article, the effect of parboiling processes on nutritional value of rice bran and evaluate nutritional value in the rumen fermentation using in vitro digestibility method was analyzed.
Abstract: Parboiled rice product has been widely produced by industry in the developing countries. The increasing of parboiled rice product will consequently increased rice bran waste. Therefore, the objective of the study was to analyze the effect of parboiling processes on nutritional value of rice bran and evaluate nutritional value of parboiled rice bran in the rumen fermentation using in vitro digestibility method. The experiment was arranged in completely randomized factorial design with 2 factors and 3 replications. The first factor is types of rice bran from different rice plant variety consisted of LIPI GO1, LIPI GO2, LIPI GO4, and Sintanur. The second factor is soaking time consist of 0 minute (control), 30 minutes and 60 minutes. Data were analyzed by using ANOVA and significant effects of each treatment were further analyzed by using the least significant difference by Duncan multiple range test (P<0.05). Kinetic parameters of Orskov’s equation were obtained by non-linear regression procedure. Results showed that the quality of parboiled rice bran was significantly affected by the parboiling process such as soaking periods and rice variety. Chemical composition, rumen gas production, and digestibility of rice bran differ depends on rice variety . Soaking for 30 minutes and 60 minutes decreased organic matter, crude protein, and increased crude fiber (P<0.05). Moreover, soaking rice grain for 60 minutes increased crude fat. The longer of soaking periods decreased of total gas, potential gas production, gas production rate and total organic matter digestibility (P<0.05).
TL;DR: In this paper, the authors used Bacillus amyloliquefacien, Trichoderma harzianum and cocktail microbes to improve the nutritive value of palm kernel cake.
Abstract: The aims of this research were to improve the nutritive value of palm kernel cake by fermentation technology using Bacillus amyloliquefacien, Trichoderma harzianum and cocktail microbes (combination of Bacillus amyloliquefacien and Trichoderma harzianum ). Data were analyzed using Completely Randomized Design Factorial, with experiments consisting of types of microbes ( B. amyloliquefaciens , T. harzianum , microbial cocktail) as treatment and incubation time (0, 3, 5, and 7 days). Parameters were crude protein and crude fiber for all treatments, and the lowest fiber analysis would continue with NDF, ADF, crude fat, and amino acids. Result showed that the three of microbes grew on palm kernel meal in third incubation and grew on and in the substrate at 7 days. microbial cocktails increased protein and reduced crude fiber better than B. amyloliquefacien and T. harzianum on palm kernel cake fermentation technology. Cocktail microbes enhanced amino acids such as methionine, arginine, and glutamic acid, also neutral detergent fiber but reduced ADF and hemicellulose. It was concluded that the palm kernel cake fermented with microbial cocktails can reduced crude fiber and increase crude protein with an incubation period of 7 days.
TL;DR: In this article, the potency of protein-bioactive of native ducks-meat after enzymatic hydrolysis by Bacillus-cereus TD5B-protease, Pepsin, and Trypsin as an angiotensin-converting enzyme (ACE) inhibitor was explored.
Abstract: This study was aimed to explore their potency of protein-bioactive of native ducks-meat after enzymatic hydrolysis by Bacillus-cereus TD5B-protease, Pepsin, and Trypsin as an angiotensin-converting enzyme (ACE) inhibitor. The samples: ducks-meats from 10 months age of male Mojosari and Magelang-Duck. The experiments: individually hydrolysis of meat-protein using protease-enzyme (0.1 % w/w) from Bacillus-cereus TD5B, Pepsin, or Trypsin. The observed parameters: protein concentration, protein molecular weight, ACE-inhibitor activity, and IC-value (IC 50 ). Data of protein concentration were statistically analyzed using T-Test, while data of SDS-PAGE and ACE-inhibiting activity were analyzed descriptively. The results showed that soluble protein concentration increased due to the hydrolysis process, from 0.826±0.108 mg/mL to 1.050±0.197 mg/mL (Microbial-protease), 2.122±0.141 mg/mL (pepsin), 1.641±0.071 mg/mL (trypsin) for Mojosari-duck and 0.642±0.038 mg/mL to 1.171±0.534 mg/mL(Microbial-protease), 2.100±0.376 mg/mL(pepsin), 1.725±0.092 mg/mL(trypsin) for Magelang-duck. The SDS-PAGE pattern showed that there was a decrease of molecular weight of duck-meats due to the hydrolysis process, from the range of 196.53-43.88 kDa to the range of 71.35-10.12 kDa. Duck-meat protein hydrolysate had ACE-inhibiting activity 71.7%(Mojosari-Microbial-Protease) IC 50 54μg/mL, 57%(Mojosari-Pepsin) IC 50 151μg/mL, 75.8%(Mojosari-Trypsin) IC 50 51μg/mL and 52.8%(Magelang-Microbial-Protease) IC 50 83μg/mL, 78,5%(Magelang-Pepsin) IC 50 85μg/mL, 83.9%(Magelang-Trypsin) IC 50 22μg/mL. In c onclusion, hydrolysate of Magelang duck-meat used Trypsin had better potency as an ACE-inhibitor.
TL;DR: Genetic distance and genetic tree determined according to sequence in hypervariability (HV-1) region of D-loop mtDNA resulted in satisfied separation, successfully classifying Bos javanicus, Bos indicus, and Bos taurus cluster.
Abstract: This study aimed to investigate the diversity of the complete sequence of D-loop mitochondrial DNA (mtDNA) in Bali and Sumba Ongole (SO) cattle breeds . A total of 24 blood samples were collected from Bali cattle (19 heads ) and SO cattle (5 heads ), and were extracted and then analyzed to obtain the sequence of D-loop mt DNA . Multiple alignments of the whole sequence of D-loop mtDNA were determined using clustal W. Genetic distance was calculated using a p-distance method, while the genetic tree was constructed using neighbor-joining (NJ) based on MEGA 6. Haplotype number, haplotype diversity (Hd) and nucleotide diversity (Pi) were analyzed using DnaSP version 6. As a result, the sequence of D-loop mtDNA in Bali cattle (921-1119 bp) and SO cattle (913 bp) was reported to have 8 and 4 haplotypes. Hd and Pi of Bali cattle reached 0.625±0.139 and 0.0266±0.0145, respectively, which wwere different from that of SO cattle, namely 0.900±0.1610 and 0.0064±0.0015, respectively. Specifically, we found 22 bp-repetitive nucleotide in Bali cattle, existing 3-9 times with a length of 66-198 bp present in D-loop mtDNA. This unique feature did not exist in SO cattle. Genetic distance and genetic tree determined according to sequence in hypervariability (HV-1) region of D-loop mtDNA (166 bp) resulted in satisfied separation, successfully classifying Bos javanicus , Bos indicus , and Bos taurus cluster.
TL;DR: In this article, the effect of simvastatin and L-carnitine (LC) additives and their interactions on productive performance and carcass quality of broilers was evaluated.
Abstract: The aim of this study was to evaluate the effect of simvastatin (SIM) and L-carnitine (LC) additives and their interactions, on productive performance and carcass quality of broilers. For this purpose, a feeding trial was conducted, under 3×3 factorial design, using SIM at 0, 1 or 2 g/kg level and LC at 0, 150 or 300 mg/kg level in a basal-diet and originating a total of nine treatments: T1 (control-diet, 0/0), T2 (0/150), T3 (0/300), T4 (1/0), T5(1/150), T6 (1/300), T7 (2/0), T8 (2/150), and T9 (2/300). Feed intake was higher in T2 (4716 g), T7 (4722 g) and T9 (4698 g) than in T1 (4545 g; P<0.05) considering the last growing phase (35-42 days) or whole 42-day production cycle. An improvement of feed efficiency was also observed in T8 (1.64) and T9 (1.67) when compared to T1 (1.77; P<0.05), and these were mainly due to SIM × LC interactions (P<0.05). The LC influenced positively ( P <0.05) the weight of eviscerated carcass, breast, drumsticks and abdominal fat, as well as plasma triglycerides level. This findings suggests that the combination of SIM and LC additives have a positive influence on growth performance and carcass traits of broiler chickens.
TL;DR: In this article, the authors identified the polymorphism of the leptin gene and its association with growth traits in Kebumen Ongole Grade cattle and found that SNP g. 1180 C>T was found in the population.
Abstract: The aim of this study was to identify the polymorphism of leptin gene and its association with growth traits in Kebumen Ongole Grade cattle. One hundred blood samples were collected for molecular analysis. Polymorphism of the leptin gene was analyzed using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) with HpyCH4V restriction enzyme and DNA sequencing. Association analysis of the leptin gene with growth traits was analyzed by T-test. The results showed that SNP g. 1180 C>T was found in the population. The SNP changed amino acid from arginine to cysteine. The SNP was significantly associated with a high chest circumference at weaning age in animal having CC genotype (P T in the leptin gene is potential as genetic marker for growth traits in Kebumen Ongole Grade cattle.
TL;DR: In this article, the authors evaluated the quality and deposition of isoflavones in the egg due to dietary inclusion of ISBP in laying hens, and found that dietary inclusion significantly decreased cholesterol and increased is oflavone in egg.
Abstract: The objective of the study was to evaluate the quality and deposition of isoflavones in the egg due to dietary inclusion of isoflavone soy sauce by-product (ISSBP) in laying hens. Experimental animals were 480 birds of 20-week old laying hens of Isa Brown strain, with initial body weight of 1,754 ± 42 g. The experiment was arranged in a completely randomized design (CRD), with four treatments and six replications (20 birds each). The treatments were dietary inclusion levels of isoflavone soy sauce by-product as follows: ISSBP0 = without isoflavones, ISSBP40 = 40 mg/100g, ISSBP80 = 80 mg/100g, and ISSBP120 = 120 mg/100 g feed. Dietary treatments were given for 10 weeks from week 20 until 30-week old. Parameters observed were egg cholesterol and isoflavone isomers in feed, blood, and eggs. Data of total cholesterol and isoflavone in the egg were statistically analysed and isoflavone isomer concentration in feed, blood, and egg were descriptively described. The results showed that feeding ISSBP significantly (P <0.05) decreased cholesterol and increased isoflavones in egg. Total cholesterol content in egg decreased up to 33.8%. However, isoflavones in the yolk were higher (28.9 mg/g) than those in blood (13.75 mg/g), and those deposited into the yolks indicated better quality because containing more aglicons isomers (87.5%), than those in feed (52%) and blood (68.4%). In conclusion, dietary inclusion of ISSBP to laying hens’s decreased cholesterol content and increased isoflavones deposition into the egg with better quality, so that the eggs can function as functional food.
TL;DR: In this article, the authors compared the percentage of Sodium Chloride (NaCl) and cervical mucus profile which includes potential of Hydrogen (pH), abundance of cervical fluid, spinnbarkeit and ferning of dairy cattle at different ages during the estrus cycle.
Abstract: Objectives of this study were to compare the percentage of Sodium Chloride (NaCl) and cervical mucus profile which includes potential of Hydrogen (pH), abundance of cervical mucus, spinnbarkeit and ferning of dairy cattle at different age during estrus cycle. Thirty Two head of dairy cattle were used as materials which are 2 years old (n = 2), 3 years old (n = 18), 4 years old (n = 8) and 5 years old (n=4). Dairy cattle were estrus synchronized using 50 mg/head of prostaglandin. Data was collected on 48, 72, 78, 84, 90, 120, 144 and 408 hours after prostaglandin administration. The data were analyzed by using non parametric statistic which was Kruskal-Wallis H test. The significant data was tested with Mann-Whitney U test. The result showed that the abundance of cervical mucus was significantly different (P<0.05; χ 2 = 0.011) on 84 hours after estrus synchronization. However, level of NaCl, abundance, spinnbarkeit and ferning of cervical mucus was not sigificantly different. In conclusion, the age of dairy cattle did not affected to the condition NaCl percentage, spinnbarkeit, pH and ferning of cervical mucus, but affected to the abundance of cervical mucus on 84 hours after adminitration of prostaglandine.
TL;DR: Saanen goats have more varieties α-casein allele than Anglo-Nubian goats, according to the breed factor that influences milk production in different goat breeds in the tropical environmental area.
Abstract: This study was completed to investigate the breed factor that influences milk production in different goat breeds in the tropical environmental area. A total of 28 goats were utilized in this work. These goats consisted of two different breeds, which were Anglo-Nubian and Saanen . Blood samples were withdrawn through jugular veins of goats into plastic tubes without EDTA for gene polymorphism. A t-test was used to analyse if there are any significant differences in molecular weight and frequency values of DNA between breeds. Investigation of DNA polymorphism in alleles A, B and C of α-casein were identified in all breeds, while allele F were studied in Saanen goats only. As a conclusion; Saanen goats have more varieties α-casein allele
TL;DR: In this paper, the automatic linear modelling (ALM) and artificial neural network (ANN) models were employed to predict hatchability and mortality rate of Muscovy ducks in Nasarawa State, Nigeria.
Abstract: This study was embarked upon to predict hatchability and mortality rate of Muscovy ducks in Nasarawa State, Nigeria. Data were obtained from a total of 119 duck farmers. The automatic linear modelling (ALM) and artificial neural network (ANN) models were employed. The average flock size was 9.84±0.60 per household. The predicted hatchability mean values using ALM (8.66) and ANN (8.65) were similar to the observed value (8.66). The predicted mortality mean values using ALM (2.95) and ANN (3.03) were also similar to the observed value of 2.95. Experience in duck rearing, the educational status of farmers, source of foundation stock and season were the variables of importance in the prediction of hatchability using ALM and ANN models. However, primary occupation, source of foundation stock, experience in duck rearing, land holding and management system were the important variables automatically selected for the prediction of mortality. Moderate coefficients of determination (R 2 = 0.422 vs 0.376) and adjusted R 2 (0.417 vs 0.371) estimates were obtained for hatchability and mortality using ALM. Different patterns were obtained under the ANN models as regards the prediction of hatchability (R 2 = 0.573 and adjusted R 2 = 0.569) and mortality (R 2 = 0.615 and adjusted R 2 = 0.612). The present information may aid management decisions towards better hatchability and mortality performance in Muscovy ducks.
TL;DR: In this article, the authors investigated the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract.
Abstract: The study aimed at investigating the population, the protein profile and the ultrastructure of adult worms in the intestine of domestic chicken treated using Areca catechu crude aqueous extract. Fifty domestic female chickens of 6 weeks of age were assigned to 5 groups. Group A (negative control) was not given any treatment and any drug. Groups B, C and D were given the treatment at the doses of 26 mg/mL, 53 mg/mL and 79 mg/mL, respectively. Group E (positive control) was given Pyrantel®. Necropsy was conducted to all of the chickens 14 days after the treatment. Adult worms were collected and counted. The worms used in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Scanning Electron Microscopy (SEM) were those collected from the jejunum of the chickens in the groups A, B and C. The biggest number of the worms was found in the jejunum. The results of electrophoresis showed that the dose 53 mg/mL gave fewer protein bands than the negative control (21:12 ratio), while the results of the SEM showed that there was cuticle damage and anterior labia abrasion at the dose of 53 mg/mL. The Areca catechu crude aqueous extract showed anthelmintic activity potential by reducing the number of the adult worms, lowering their protein profile and damaging the A. galli worms in the intestine.
TL;DR: There was no association between β-LG genotype and milk protein and milk production of Saanen goats.
Abstract: The objectives of this study were to analyze and identify the β-LG genotype and examine its association with milk protein and milk production of Saanen goats. Materials used in this study were DNA that originated from 22 Saanen does and its milk protein and milk production recording that was taken at 2,5; 3,0; 3,5 and 4,0 years old of Saanen does. DNA was analyzed using PCR-RFLP method with SacII restriction enzyme to identify genotype of B-LG gene and then were sequenced for each genotype. Sequencing data was analyzed using Clustal W in MEGA7.0, while association of B-LG gene genotype with milk protein and milk production was analyzed using General Linear Model (GLM) in SAS 9.0. The results showed that two alleles (A and G), forming two homozygous genotypes (AA and GG) and one heterozygous genotype (AG) were found, but there were no significant different within genotype on milk protein and milk production. In conclusion, there was no association between β-LG genotype and milk protein and milk production of Saanen goats.
TL;DR: In this article, a study was conducted to analyze the broiler farmers' capacity level in the partnership pattern and analyze the factors which affect it. But the results of the study revealed that the farmers' business capacity was weak.
Abstract: The present study was conducted to analyze the broiler farmers’ capacity level in the partnership pattern and to analyze the factors which affect it. The current study was conducted in Bogor and Sukabumi, West Java Province using a survey approach on 247 farmers who ran farm businesses with the partnership pattern. Data were collected through questionnaire filling by farmers and in-depth interviews with key informants. The data were collected from July to October 2018. The data were analyzed descriptively and with inferential analysis (regression). The results of the study revealed that the farmers’ business capacity was weak. This was evident in the managerial and partnership capability which was categorized as low, the entrepreneurship and problem-solving capability which was categorized as medium, and only the technical capability which was categorized as high. The regression results demonstrated that business capacity was influenced positively by the formal education level, social environment characteristics (organizational support, access to experts), and the role of private extension agents (their role as educators, communicators, consultants, dynamizators, and organizers).
TL;DR: In this article, the authors evaluated chemical composition, in vitro fermentation and digestibility of dried and ensiled indigofera, papaya and moringa leaves, and concluded that ensiling of high protein forages leads to considerable extent of proteolysis.
Abstract: The objective of this experiment was to evaluate chemical composition, in vitro fermentation and digestibility of dried and ensiled indigofera, papaya and moringa leaves. The leaves were subjected to artificial drying in an oven at 60 o C for 24 h and ensiling treatment for 30 d under room temperature. Dried and ensiled samples were determined for chemical composition, silage fermentation characteristics and in vitro rumen fermentation and digestibility. The experimental design was a factorial design 3 × 2 in which the first factor was different leaves (indigofera, papaya and moringa) and the second factor was conservation treatments (drying and ensiling). Determination of chemical composition was performed in duplicate whereas in vitro evaluation was conducted in three replicates. Results showed that ensiling treatment decreased CP contents of indigofera and moringa but not papaya leaves. Ensiling also decreased NDF and NDICP contents of all experimental leaves in comparison to drying treatment. The pH of all silages was high and they were characterized with high ammonia concentrations. Ensiled indigofera tended to have lower IVDMD and IVOMD as compared to dried indigofera (P<0.1). It can be concluded that ensiling of high protein forages leads to considerable extent of proteolysis.
TL;DR: In conclusion, the feed could be restricted to 90% ad libitum with a 2900 kcal/kg ration of ME and fed during the starter period (14 to 42 days of age) without influencing ovarian morphology in quails.
Abstract: The aim of this research was to evaluate the effect of feed restriction on growth hormone profiles and ovarian morphology during the growth period. Three hundred 14-day-old quails were used. The quails were maintained on two feeding restriction programs: two dietary regimes based on metabolizable energy (ME), R1 = 2900 kcal/kg and R2 = 2800 kcal/kg, and 3 quantitative feed restriction diets, P0 = 100% ad libitum ; P1= 90% ad libitum and P2 = 80% ad libitum (n=300). Each group (n=50) was processed with five replications, 10 birds in each replicate. The change in growth hormone was determined at 28, 35, 42, and 49 days of age, while ovarian morphology was determined at sexual maturity. The results indicated that feed restriction induced a significant increase in growth hormone and insulin-like growth factor-1 after re-feeding. There was no significant effect caused by the rationing of metabolizable energy. The number of large yellow follicles was not different between quails fed with 100% ad libitum and 90% ad libitum . However, feed restriction significantly increased the number of small yellow follicles. In conclusion, t he feed could be restricted to 90% ad libitum with a 2900 kcal/kg ration of ME and fed during the starter period (14 to 42 days of age) without influencing ovarian morphology in quails.
TL;DR: The study showed that supplementation with tannin-containing forages could improve feed consumption and the digestibility of dry matter (DM), organic matter (OM), and crude protein (CP) and supplementation improved the daily body weight gain.
Abstract: This study investigated the nutritional potency and efficacy of tannin-containing forages as anti-parasites sources to support the performance of female Bligon goats. Fifteen female Bligon goats were divided into 3 different groups based on feeding treatments. The first group (KG) served as the control and was fed 100% king grass (dry matter basis). The second group (KGC) was fed 50% king grass and 50% Calliandra calothyrsus leaves (dry matter basis). The third group (KGA) was fed 50% king grass and 50% Artocarpus heterophyllus leaves (dry matter basis). The study showed that supplementation with tannin-containing forages could improve feed consumption and the digestibility of dry matter (DM), organic matter (OM), and crude protein (CP). The groups that received tannin-containing forages had higher body weight gains at 22.85 g/day and 29.52 g/day for KGC and KGA respectively than the control group. The anti-parasites effects were successfully indicated by the declining number of coccidian oocysts in the feces (number/gram feces), which were 3,166; 841; and 450 for KG, KGC, and KGA respectively. Tannin-containing forage supplementation could improve the consumption and digestibility of DM, OM and CP. Supplementation improved the daily body weight gain. The number of coccidian oocysts in animals receiving supplementation was also reduced. The greatest effects on fecal coccidian oocyst reduction were attained with Jackfruit leaves ( Artocarpus heterophyllus ) supplementation