Showing papers in "Journal of The Korean Association of Oral and Maxillofacial Surgeons in 2005"

멍게와 미더덕 피부의 천연 셀룰로오스 각질을 이용한 골재생 효능을 가진 생활성막의 개발 - 예비 연구

Abstract: Objectives: To develop a bioactive membrane for guided bone regeneration (GBR), the biocompatibility and bone regenerating capacity of the cellulose membrane obtained from the Ascidians squirt skin were evaluated. Materials and methods: After processing the pure cellulose membrane from the squirt skin, the morphological study, amino acid analysis and the immunoreactivity of the cellulose membrane were tested. Total eighteen male Sprague-Dawley rats (12 weeks, weighing 250 to 300g) were divided into two control (n=8) and another two experimental groups (n=10). In the first experimental group (n=5), the cellulose membrane was applicated to the 8.0 ㎜ sized calvarial bone defect and the same sized defect was left without cellulose membrane in the first control group (n=4). In the another experimental group (n=5), the cellulose membrane was applicated to the same sized calvarial bone defect after femoral bone graft and the same sized defect with bone graft was left without cellulose membrane in the another control group (n=4). Each group was sacriticed after 6 weeks, the histological study with H&E and Masson trichrome stain was done, and immunohistochemical stainings of angiogenin and VEGF were also can-ied out. Results: The squirt skin cellulose showed the bio-inductive effect on the bone and mesenchymal tissues in the periosteum of rat calvarial bone. This phenomenon was found only in the inner surface of the cellulose membrane after 6 weeks contrast to the outer surface. Bone defect covered with the bioactive cellulose membrane showed significantly greater bone formation compared with control groups. Mesenchymal cells beneath the inner surface of the bioactive cellulose membrane were positive to the angiogenin and VEGF antibodies. Conclusion: We suppose that there still remains extremely little amount of peptide fragment derived from the basement membrane matrix proteins of squirt skin, which is a kind of anchoring protein composed of glycocalyx. This composition could prevent the adverse immunological hypersensitivity and also induce bioactive properties of cellulose membrane. These properties induced the effective angiogenesis with rapid osteogenesis beneath the inner surface of cellulose membrane, and so the possibilities of clinical application in dental field as a GBR material will be able to be suggested.

2D and 3D structural study of rete ridge in oral mucosa and skin paddle of various free flaps

Abstract: J. Kor. Oral Maxillofac. Surg. 2005;31:143-149)

Sciatic nerve regeneration using calcium phosphate coated conduit and brain-derived neurotrophic factor gene-transfected schwann cell in rat.

Abstract: J. Kor. Oral Maxillofac. Surg. 2005;31:199-218) 이 종 호 110-768 서울시종로구연건동275-1 서울대학교치과대학구강악안면외과학교실 Jong-Ho Lee Dept. of OMFS, College of Dentistry, Seoul National University #275-1 Yeongun-dong, Jongno-gu, Seoul, 110-768, South Korea Tel: 82-2-2072-2630 Fax: 82-2-766-4948 E-mail: leejongh@plaza.snu.ac.kr ※본연구는보건복지부보건의료기술진흥사업의지원에의하여이루어진것임(과제고유번호: 02-PJ1-PG1-CH07-0001) SCIATIC NERVE REGENERATION USING CALCIUM PHOSPHATE COATED CONDUIT AND BRAIN-DERIVED NEUROTROPHIC FACTOR GENE-TRANSFECTED SCHWANN CELL IN RAT Won-Jae Choi, Kang-Min Ahn, Soon-Jeong Hwang, Pill-Hoon Choung, Myung-Jin Kim, Nam-Yeol Kim, Sang-Bae Yoo, Jeong-Won Jahng, Hyun-Man Kim, Joong-Soo Kim, Yun-Hee Kim, Soung-Min Kim, Seung-Jae Oh, Sung-Jun Kim, Byoung-Eun Yang, Jong-Ho Lee Department of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University Department of Pharmacology, Yonsei Medical School Department of Oral Anatomy, College of Dentistry, Seoul National University Department of Oral Physiology, College of Dentistry, Seoul National University Department of Biology, Kyung-Hee University Department of Oral & Maxillofacial Surgery, College of Dentistry, Kangnung National University School of Electrical Engineering and Computer Science, Seoul National University Department of Oral & Maxillofacial Surgery, College of Medicine, Hallym University Purpose of Study: Peripheral nerve regeneration depends on neurotrophism of distal nerve stump, recovery potential of neuron, supporting cell like Schwann cell and neurotrophic factors such as BDNF. Peripheral nerve regeneration can be enhanced by the conduit which connects the both sides of transected nerve. The conduit maintains the effects of neurotrophism and BDNF produced by Schwann cells which can be made by gene therapy. In this study, we tried to enhance the peripheral nerve regeneration by using calcium phosphate coated porous conduit and BDNF-Adenovirus infected Schwann cells in sciatic nerve of rats. Materials and Methods: Microporous filter which permits the tissue fluid essential for nerve regeneration and does not permit infiltration of fibroblasts, was made into 2mm diameter and 17mm length conduit. Then it was coated with calcium phosphate to improve the Schwann cell adhesion and survival. The coated filter was evaluated by SEM examination and MTT assay. For effective allogenic Schwann cell culture, dorsal root ganglia of 1-day old rat were extracted and treated with enzyme and antimitotic Ara-C. Human BDNF cDNA was obtained from cDNA library and amplified using PCR. BDNF gene was inserted into adenovirus shuttle vector pAACCMVpARS in which E1 was deleted. We infected the BDNF-Ad into 293 human mammary kidney cell-line and obtained the virus plaque 2 대구외지 2005;31:199-218

PMMA microspheres (ARTECOLL(R)) injection for nasal ridge augmentation in the orthognathic surgery.

Abstract: J. Kor. Oral Maxillofac. Surg. 2005;31:329-334) 김 명 진 110-744 서울특별시종로구연건동 28 서울대학교치과대학구강악안면외과학교실 Myung-Jin Kim Dept. of OMFS, College of Dentistry, Seoul National University 28 Yeongun-Dong, Chongro-Gu, Seoul, 110-744, Korea Tel: 82-2-2072-2632 Fax: 82-2-766-4948 E-mail: myungkim@plaza.snu.ac.kr PMMA MICROSPHERES (ARTECOLL) INJECTION FOR NASAL RIDGE AUGMENTATION IN THE ORTHOGNATHIC SURGERY Yong-Ju Ok, Myung-Jin Kim, Jun-Young Paeng, Hoon Myoung, Soon-Jung Hwang, Jin-Young Choi, Jong-Ho Lee, Pill-Hoon Choung Department of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University Polymethyl-methacrylate(PMMA; Artecoll) microspheres suspended 1 : 3 in a 3.5% collagen solution has been used as an injectable implant for long lasting correction of wrinkles and minor skin defects. The patients with mandibular prognathism have increased necessity for nasal augmentation. Usually these patients usually get an additional rhinoplasty after orthognathic surgery. The purpose of this study is to evaluate the result of PMMA injection for nasal ridge augmentation simultaneously with the orthognathic surgery. PMMAs were injected to the nasal dorsum of 13 patients with mandibular prognathism to augment the nasal ridge at the end of the orthognathic surgery. The cephalometric X-ray and clinical facial photograph were taken at 2, 4 and 6 months after operation. Using S-N line, we calculated the change of soft tisuue on the nasal ridge and also investigated the degree of patients satisfaction at 6 months after operation. Most of the patients were satisfied with their nasal ridge height status from moderate to good degree. The average amount of nasal ridge augmentation was 1.4 ± 0.5 mm immediately after operation, 1.2 ± 0.4 mm at 2 months after operation. The postoperative nasal ridge height seemed to be remained stable after 2 months. Intraoperative PMMA injection is considered to be simple and effective technique which can be used for the minor augmentation of nasal ridge in the orthognathic patients.