Showing papers in "Vìsnik Dnìpropetrovsʹkogo Unìversitetu: Serìâ Bìologìâ, Ekologìâ in 2010"

Membrane erythrocyte proteins changes under noncalculous cholecystitis and crohn’s disease

Abstract: The aim of the investigation was to reveal the changes in erythrocytes’ proteins from human blood under gastroenterological pathologies, namely noncalculous cholecystitis and Crohn’s disease. The erythrocyte was used as a model system. The PAAG electrophoresis with SDSNa method was used. Erythrocyte membrane proteins quantitative changes were observed in patient groups under liver and intestines diseases. High molecular weight proteins content (spectrines α and β) was reduced, but any changes were not registered under the Crohn’s disease. Low molecular weight proteins content (anion-transporting protein and protein band 4.5) increased considerably as compared with a control (healthy people group). The structure of erythrocytes’ membrane changed, that led to disturbance of function of cytoskeleton proteins. The research results give more wide explanations of the pathologies genesis.

Microbiological aspects of probiotic preparations

Abstract: The main stages of the beginning and development of probiotic preparations, the general characteristics of modern probiotics, the scope of their use in medicine and veterinary are presented. The characteristics of microorganisms (bifidus bacteria, lactobacteria and Bacillus bacteria) used in the production of probiotics, the basic requirements that should be met by these organisms, as well as prospects for the development of probiotics are shown.

Catalase and superoxiddismutase activities in Desulfovibrio desulfuricans Ya-11 under the influence of heavy metals salts

Abstract: The highest catalase activity (42.67×10 – 2 µM×min – 1 ×mg – 1 of protein) in the cells of Desulfovibrio desulfuricans Ya-11 has been observed under the prolonged Pb(NO 3 ) 2 influence. In the presence of other heavy metals’ salts it has been changed in dependence on their concentrations and growth duration. Based on our research data the row of heavy metals’ salts by its influence on D. desulfuricans Ya-11 catalase activity has been formed at first: Pb(NO 3 ) 2 > CuCl 2 > CdCl 2 > ZnCl 2 . The highest superoxide dismutase activity (61.52×10 – 2 µM×min – 1 ×mg – 1 of protein) has been observed under the prolonged ZnCl 2 influence. In the presence of other heavy metals’ salts this enzyme’s activity increased with increasing the salts’ concentrations. Based on our According to our results, the row of heavy metals’ salts influence on D. desulfuricans Ya-11 superoxide dismutase activity has been formed at first: ZnCl 2 > Pb(NO 3 ) 2 > CuCl 2 > CdCl 2 .

Food, medicinal and environmental values of mushrooms Pleurotus ostreatus

Abstract: We present the literature review describing food, medicinal and ecological properties of the fungus Pleurotus ostreatus (oyster mushroom). It is shown that the mushroom is adequate foodstuff for human beings. It provides with proteins, carbohydrates, fats, vitamins and mineral salts. Protein of the oyster mushrooms’ mycothallus contains 18 amino acids, eight of which were essential (isoleucine, leucine, lysine, methionine, phenylalanine, tryptophan, threonine, and valine). Therapeutic value of the mushroom is characterised by a content of water-soluble (thiamine B1 , riboflavin B2 , niacin, B5 , PP , pyridoxine B6 , biotin B7 , ascorbic and pantothenic acid) and liposoluble (calciferol, ergosterol, tocopherol) vitamins. The considerable gains from the farm wastes use for the mushrooms raising with subsequent application of the substrate in plant cultivation and animal husbandry are stated.