Figure 1: Effects of 20E on protein synthesis and Myostatin gene expression in C2C12 cells. (A) Experiments displaying 20E effects on protein synthesis in differentiated myotubes detected by [3H]-leucin incorporation. Results are shown as means ± SEM, ***p < 0.001, **p < 0.01, *p < 0.05 vs untreated control (Kruskal-Wallis followed by a Dunn’s test). (B) C2C12 mouse myoblasts were differentiated for 6 days into myotubes. They were then treated for 6 hours with concentrations of 20E ranging from 0.001 to 10 μM. Myostatin gene expression was detected by qRT-PCR. Results are shown as means ± SEM with ***p<0.001, **p<0.01, *p<0.05 vs untreated control (one-way ANOVA with Dunnett’s test compared to untreated control).
...read more