Figure 2: 20E acts on C2C12 myotubes from outside of the cell. C2C12 mouse myoblasts were differentiated for 6 days into myotubes. They were then treated for 6 hours with IGF-1 (100 nM), 20E (10 μM) or 20E-HSA (10 μM 20E-equivalent). Myostatin gene expression was detected by qRT-PCR. Results are shown as means ± SEM with *p < 0.05 vs untreated control (one-way ANOVA with Dunnett’s test compared to untreated control).
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