Patent
5- and 6- Succinimidyl - carboxylate isomers of rhodamine dyes
Steven M. Menchen,Steven Fung +1 more
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Isomerically pure 5-and 6-succinimidylcarboxylates of rhodamine dyes are provided in this paper, which prevents generation of spurious sequence data because of the different electrophoretic mobilities of the isomers.Abstract:
Isomerically pure 5- and 6-succinimidylcarboxylates of rhodamine dyes are provided. A key feature in the preparation of the succinimidylcarboxylates is the use of stoichiometric amounts of di-N-succinimidylcarbonate and 4-dimethylaminopyridine for converting the 5- and/or 6-carboxyrhodamines to N-hydroxysuccinimide esters. In chain termination DNA sequencing procedures, using the pure isomeric forms of the activated rhodamines prevents generation of spurious sequence data because of the different electrophoretic mobilities of the isomers.read more
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Methods and products for analyzing polymers
TL;DR: In this paper, methods and products for analyzing polymers are provided. The methods include methods for determining various other structural properties of the polymers, such as properties of polymers' properties and properties of their properties.
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Method of detecting electrophoretically separated oligonucleotides
TL;DR: In this article, a method for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis is presented, which consists of derivatives of fluorescein, 2',7'-dimethoxy-4', 5'-dichlorofluoresceins, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively.
Patent
High eicosapentaenoic acid producing strains of Yarrowia lipolytica
Howard Glenn Damude,Peter J. Gillies,Daniel Joseph Macool,Stephen K. Picataggio,Dana M. Walters Pollak,James John Ragghianti,Zhixiong Xue,Narendra S. Yadav,Hongxiang Zhang,Quinn Qun Zhu +9 more
TL;DR: In this article, engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an (ω-3 polyunsaturated fatty acid) in the total oil fraction are described.
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Production of polyunsaturated fatty acids in oleaginous yeasts
TL;DR: In this paper, a method for the production of omega-3 and/or omega-6 fatty acids in oleaginous yeast was proposed, which relates to methods for the synthesis of omega 3 and omega 6 fatty acids.
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Charge switch nucleotides
John G. K. Williams,Gregory R. Bashford,Jiyan Chen,Dan Draney,Nara Narayanan,Bambi L. Reynolds,Pamela Sheaff +6 more
TL;DR: In this paper, a single-molecule DNA sequencing in a micro-channel sorting system is realized, enabling real-time sequencing as successive detectable moieties flow through a detection channel.
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Polysubstituted aryl acridinium esters
TL;DR: In this article, aryl acridinium esters are used as luminescent labels for specific binding assays such as immunoassays or nucleic acid hybridization assays.
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Pyrylium dyes having a fused,rigidized nitrogen-containing ring
TL;DR: In this paper, a class of pyrylium dyes, having at least one rigidized, nitrogen-containing heterocyclic ring fused thereto, is described, and the rigidized dyes are useful lasing components in lasing media.
Patent
Chromable dyestuffs of the triarylmethane series and process of preparing them
Karl Schilling,Wilhelm Eckert +1 more
TL;DR: In this paper, it was shown that very clear dyestuffs of the triarylmethane series capable of being chromed are obtainable by condensing one molecular proportion of the anhydride of 5-hydroxybenzene.
Patent
Chromable dyestuffs of the triarylmethane series and a process of preparing them
Karl Schilling,Wilhelm Eckert +1 more
TL;DR: In this article, it was shown that chromable dyestuffs of the triarylmethane series are obtainable by condensing one molecular proportion of 5-sulfobenzene1.2.4-tricarboxylic acid or of the isomeric-...