S
Steven M. Menchen
Researcher at Applied Biosystems
Publications - 68
Citations - 2288
Steven M. Menchen is an academic researcher from Applied Biosystems. The author has contributed to research in topics: Rhodamine & Polynucleotide. The author has an hindex of 22, co-authored 68 publications receiving 2280 citations. Previous affiliations of Steven M. Menchen include PerkinElmer & Life Technologies.
Papers
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Journal ArticleDOI
New dye-labeled terminators for improved DNA sequencing patterns
Barnett B. Rosenblum,Linda G. Lee,S. L. Spurgeon,Shaheer H. Khan,Steven M. Menchen,C. R. Heiner,S. M. Chen +6 more
TL;DR: The improved signal-to-noise ratio of the energy-transfer dye-labeled terminators combined with more even peak heights results in successful sequencing of high molecular weight DNA templates such as bacterial artificial chromosome DNA.
Patent
4,7-dichlorofluorescein dyes as molecular probes
Steven M. Menchen,Linda G. Lee,Charles R. Connell,N. Davis Hershey,Vergine Chakerian,Sam Lee Woo,Steven Fung +6 more
TL;DR: In this paper, a long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances, and methods and kits for using the dyes in DNA analysis are provided.
Patent
Spectrally resolvable rhodamine dyes for nucleic acid sequence determination.
John Bergot,Vergine Chakerian,Charles R. Connell,J. Scott Eadie,Steven Fung,Davis Hershey,Linda Lee,Steven M. Menchen,Sam Lee Woo +8 more
TL;DR: In this paper, a spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. But they are not suitable for the use of DNA sequences.
Journal ArticleDOI
New energy transfer dyes for DNA sequencing
Linda G. Lee,Sandra L. Spurgeon,C. R. Heiner,Scott C. Benson,Barnett B. Rosenblum,Steven M. Menchen,Ronald J. Graham,A. Constantinescu,Krishna G. Upadhya,Jonathan M. Cassel +9 more
TL;DR: A set of four energy transfer dyes are synthesized and demonstrated their use in automated DNA sequencing and their values were reduced by 20-25% compared with the dichlororhodamine dyes alone.
Patent
Method of detecting electrophoretically separated oligonucleotides
TL;DR: In this article, a method for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis is presented, which consists of derivatives of fluorescein, 2',7'-dimethoxy-4', 5'-dichlorofluoresceins, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively.