A case of liver cystis wlud Em18-WB was useful for differential diagnosis
Takanori Aoki,Shuichi Kino,Hirotaka Yamazaki,Mitsuhiro Obara,Shinichi Kasai,Hiroshi Yamasaki,Akira Ito +6 more
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TLDR
Western blotting using Em18 antigen could greatly facilitate the differential diagnosis of simple liver cyst and alveolar hydatid disease.Abstract:
A 72-year-old woman with multilocular liver cysts was admitted. This lesions seemed to be an alveolar hydatid disease. Two methods of Western blotting were used for serologic diagnosis. One method recognizes antigens of crude extracts of Echinococcus multilocularis (EM). The other method recognizes a purified antigen (Em18) of EM. Her serum only reacted with the former method and never reacted with Em18 antigen. Eighteen months after first admission, she had an operation. The resected specimen was diagnosed with simple cysts. Western blotting using Em18 antigen could greatly facilitate the differential diagnosis of simple liver cyst and alveolar hydatid disease.read more
Citations
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Echinococcosis: serological detection of patients and molecular identification of parasites
TL;DR: Recombinant antigens Em18 and Antigen B8/1 are highly recommended for patient screening and identification of AE and CE, respectively, in combination with imaging techniques.
Journal ArticleDOI
Significance of Molecular Diagnosis using Histopathological Specimens in Cestode Zoonoses
TL;DR: In this paper, a review describes cestode zoonosis cases in which mitochondrial DNA analysis was useful not only for routine and retrospective diagnosis, but also for genetic polymorphism analysis and molecular identification of the species associated with pathogenicity.
Journal ArticleDOI
Nothing is perfect! Trouble-shooting in immunological and molecular studies of cestode infections.
TL;DR: This personal review focuses on ways to approach and overcome some of the more common issues encountered while studying cestode zoonoses, and whether developing countries should develop their own diagnostic tests or rely on commercially available kits.
Review Introduction to the symposium on cestode zoonoses in Asia and the Pacific at the 21st Pacific Science Congress Significance of Molecular Diagnosis using Histopathological Specimens in Cestode Zoonoses
TL;DR: This review describes cestode zoonosis cases in which mitochondrial DNA analysis was useful not only for routine and retrospective diagnosis, but also for genetic polymorphism analysis and molecular identification of the species associated with pathogenicity.
References
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Journal ArticleDOI
Immunodiagnosis of Echinococcus infections: confirmatory testing and species differentiation by a new commercial Western Blot.
Martine Liance,Veronique Janin,Solange Bresson-Hadni,Dominique-Angèle Vuitton,R. Houin,Renaud Piarroux +5 more
TL;DR: This study shows the usefulness of the commercially available Echinococcus Western Blot IgG for the serological confirmation of human echinococcosis and the occurrence of cross-reactivities with neurocysticercosis indicates the necessity for retesting sera with species-specific antigens, for rare patients with neurologic disorders.
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Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (EM18).
Akira Ito,Liang Ma,P.M. Schantz,Bruno Gottstein,Yue-Han Liu,J J Chai,S K Abdel-Hafez,N Altintas,D D Joshi,Marshall W. Lightowlers,Z. Pawlowski +10 more
TL;DR: Findings indicate that antigen B (8-kD) is not species- specific for E. granulosus but is genus-specific for Echinococcus, and that the Em18 antigen is a reliable serologic marker for species-specific differentiation of AE from CE.
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Immunodiagnostic and molecular approaches for the detection of taeniid cestode infections.
Akira Ito,Philip S. Craig +1 more
TL;DR: The most recent advances in techniques and applications for the detection of taeniid cestode-infected persons or animals and the use of molecular approaches for strain identification and control of parasite transmission are summarized.
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Alveolar Echinococcosis: Characterization of Diagnostic Antigen Em18 and Serological Evaluation of Recombinant Em18
Yasuhito Sako,Minoru Nakao,Kazuhiro Nakaya,Hiroshi Yamasaki,Bruno Gottstein,Marshall W. Lightowers,Peter M. Schantz,Akira Ito +7 more
TL;DR: An antigenic relationship is identified between Em18 and a 65-kDa immunodominant E. multilocularis surface protein previously identified as either EM10 or EmII/3, revealing it to be a fragment of EM10.
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Perspective on control options for Echinococcus multilocularis with particular reference to Japan
TL;DR: Views on control options are discussed with reference to different epidemiological situations of alveolar echinococcosis, considered the most serious parasitic zoonosis in temperate and arctic regions of the northern hemisphere.