A cellular protein, activating transcription factor, activates transcription of multiple E1A-inducible adenovirus early promoters
Kevin A.W. Lee,Tsong-Yuing Hai,Lakshmi Sivaraman,Bayar Thimmappaya,Helen C. Hurst,Nicolas C. Jones,Michael R. Green +6 more
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TLDR
It is demonstrated here that E4F1 and E2A-EF have identical DNA-binding specificity, which indicates a significant role for ATF in E1A-mediated transcriptional activation.Abstract:Â
We have examined the relationship between sequence-specific DNA-binding proteins that activate transcription of E1A-inducible adenovirus early promoters. Factors previously referred to as E4F1 and E2A-EF bind to the E4 and E2A promoters, respectively. We demonstrate here that E4F1 and E2A-EF have identical DNA-binding specificity. Moreover, E4F1 and E2A-EF both activate transcription of the E4 and E2A promoters in vitro. These findings demonstrate that E4F1 and E2A-EF are the same factor, which we have designated activating transcription factor, or ATF. In addition to the E4 and E2A promoters, ATF binds to an important functional element of the E1A-inducible E3 promoter. Interaction of a common activator protein, ATF, with multiple E1A-inducible early viral promoters, suggests a significant role for ATF in E1A-mediated transcriptional activation.read more
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References
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Journal ArticleDOI
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
TL;DR: A procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters, including tRNA and Ad 2 VA, is developed.
Journal ArticleDOI
Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
TL;DR: The studies indicate that transcriptional regulation of the somatostatin gene by cAMP requires protein kinase 2 activity and may depend upon a highly conserved promoter element.
Book ChapterDOI
Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
TL;DR: This chapter describes the analysis of transcription maps of polyoma virus-specific RNA by 2-D nuclease S1 gel mapping and the standard procedures used for the preparation of viral nucleic acids and hybridization probes.
Journal ArticleDOI
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region
TL;DR: Dissociation rate measurements indicate a cooperative interaction between USF and TFIID when simultaneously bound to the promoter DNA.
Journal ArticleDOI
An adenovirus type 5 early gene function regulates expression of other early viral genes
N. Jones,Thomas Shenk +1 more
TL;DR: An adenovirus type 5(Ad5) early gene function located in early region 1 which is required for the production of early cytoplasmic mRNAs corresponding to early regions 2, 3, and 4 is identified.