Journal ArticleDOI
Amino acid incorporation in vitro into protein of neuronal and glial cell-enriched fractions.
C. Blomstrand,A. Hamberger +1 more
TLDR
The suppression of amino acid incorporation was more marked for the neuronal fraction than for the glial fraction during incubation in relative hypoxia, and an increase of potassium concentration in the incubation medium enhanced the amino acids incorporation in both fractions.Abstract:
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Slices of rabbit cerebral cortex were incubated in the presence of labelled amino acids. Following incubation, neuron- and gliaenriched fractions were obtained by density gradient centrifugation and the TCA-insoluble radioactivity determined. The protein-bound radioactivity was five to six times higher in the neuronal-enriched fraction than in the glial-enriched fraction after incubation with tritiated leucine. The neuronal fraction incorporated also a number of other amino acids to a higher extent than the glial fraction (neuron/glia ratio 2·5-6). A definite dependence of incorporation on the rate of oxygenation was demonstrated. The suppression of amino acid incorporation was more marked for the neuronal fraction than for the glial fraction during incubation in relative hypoxia. An increase of potassium concentration in the incubation medium enhanced the amino acid incorporation in both fractions. Low sodium levels decreased the incorporation. Puromycin inhibited incorporation to approximately 30 per cent of control for both fractions. Addition of cycloheximide and dinitrophenol resulted in greater inhibition of incorporation in the neuronal fraction than in the neuroglial fraction. Actinomycin D did not markedly affect the incorporation in any fraction. These results are discussed in relation to in vivo and in in vitro differences for transport and incorporation of amino acids.read more
Citations
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Journal ArticleDOI
Glutamic acid as a synaptic transmitter in the nervous system. A review.
Journal ArticleDOI
Glial cell function: Active control of extracellular K+ concentration
TL;DR: The glial cell Na+ K+-ATPase was always more active, and markedly more sensitive to variations in the K+ ion concentration than the neuronal ATPase, which support the idea that control of the extracellular K- ion concentration in the CNS is at least in part dependent on the metabolically coupled sodium-potassium pump of the glia.
Journal ArticleDOI
Composition of gangliosides and phospholipids of neuronal and glial cell enriched fractions.
A. Hamberger,L. Svennerholm +1 more
TL;DR: Fractions enriched in neuronal cell bodies and in glial cells were isolated from rabbit cerebral cortex by discontinuous gradient centrifugation and the ratio of total lipid to protein was approx.
Journal ArticleDOI
Astrocytes and stroke: networking for survival?
Michelle F. Anderson,Fredrik Blomstrand,Christian Blomstrand,Peter S. Eriksson,Michael Nilsson +4 more
TL;DR: A detailed understanding of the astrocytic response, as well as the timing and location of the changes, is necessary to develop effective treatment strategies for stroke patients.
Journal ArticleDOI
Potassium-stimulated γ-aminobutyric acid release from neurons and glia
Åke Sellström,Anders Hamberger +1 more
TL;DR: The potassium- Stimulated [ 3 H]GABA release was enhanced when superfusion was performed in the presence of10 −5 M ouabain and the KCl-stimulated release was saturable at the level of 60–100% stimulation.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Preparation of enriched fractions from cerebral cortex containing isolated, metabolically active neuronal and glial cells.
TL;DR: The separation of intact metabolically active neuronal and glial cells in bulk from rat cerebral cortex depended on dispersion of the tissue in a Ficoll medium followed by centrifugation on a discontinuous FIColl gradient, and the neuronal fraction was richer than the glial in all except phospholipid.
Journal ArticleDOI
Protein turnover in cell-enriched fractions from rabbit brain.
C. Blomstrand,A. Hamberger +1 more
TL;DR: The nerve cells had one rapid and one slow phase in decline of radioactivity, while the glial cells were characterized by a more uniform decline, and certain differences between the two were observed.
Journal ArticleDOI
Lactic acid assay with L(plus)lactic acid dehydrogenase from rabbit muscle.
TL;DR: Lactate assay with L(+)lactic acid dehydrogenase from rabbit muscle and with DPN involved the problem of quantitatively reducing lactate to pyruvate, and hydrazine was found to be preferable to semicarbazide since it appeared to bind pyruVate more effectively.