Patent
Auto focus for a flow imaging system
Reads0
Chats0
TLDR
In this article, a pair of optical gratings are used to modulate light from an object, and the modulated light from either optical is used to determine the velocity of the object.Abstract:
A pair of optical gratings are used to modulate light from an object, and the modulated light from either optical is used to determine the velocity of the object. Each optical grating is offset from a reference focal point by the same distance, one grating being offset in a positive direction, the other in a negative direction. Signals produced in response to the modulated light can be processed to determine a direction in which a primary collection lens should be moved in order to improve a focus of the imaging system on the object. The lens is moved incrementally in the direction so determined, and the process is repeated until an optimal focus is achieved. In a preferred embodiment, the signals are weighted, so that the optical grating disposed closest to the optimal focus position contributes the most to velocity detection.read more
Citations
More filters
PatentDOI
Extended depth of field imaging for high speed object analysis
William E. Ortyn,David Basiji,Keith Frost,Luchuan Liang,Richard Bauer,Brian E. Hall,David J. Perry +6 more
TL;DR: In this article, an optical distortion element is introduced into the flow imaging system, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field.
Patent
Blood and cell analysis using an imaging flow cytometer
William E. Ortyn,David Basiji,Philip J. Morrissey,Thaddeus C. George,Brian E. Hall,Cathleen A. Zimmerman,David J. Perry +6 more
TL;DR: In this article, multimodal/multispectral images of a population of cells are simultaneously collected and morphometric and/or morphometric features identifiable in the images are used to separate the population into a plurality of subpopulations.
Patent
Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
Dale Buermann,John A. Moon,Bryan Crane,Mark Wang,Hong Stanley S,Jason Harris,Matthew Hage,Mark J. Nibbe +7 more
TL;DR: In this article, the authors describe a detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality in a common plane, and a translation stage configured to move the read head along a substrate that is in the common plane.
Patent
Methods and apparatus for determining particle characteristics by measuring scattered light
TL;DR: An instrument for measuring the size and characteristics of a particle contained in a sample of particles is described in this article. But this instrument is limited to the case where the particle sample is subjected to centrifugal forces so that large particles travel in the sample chamber at velocities greater than small particles.
Patent
Detection of circulating tumor cells using imaging flow cytometry
TL;DR: In this paper, the authors use morphometric and morphometric features derived from multi-mode images of cells in flow to determine if a marker corresponding to a cancer cell or precancerous cell is present in the population of cells imaged.
References
More filters
Patent
Imaging and analyzing parameters of small moving objects such as cells
David Basiji,William E. Ortyn +1 more
TL;DR: In this article, a time delay and integration (TDI) detector is used to collect and scatter light from an object moving through an imaging system, producing both scatter and dispersed images at different locations on one or more TDI detectors.
Patent
Methods and apparatus for DNA sequencing
TL;DR: In this paper, a method and apparatus for automated DNA sequencing is described, which includes the steps of: a) using a processive exonuclease to cleave from a single DNA strand the next available single nucleotide of the strand; b) transporting the single nucleote away from the DNA strand; c) incorporating the single nucleus in a fluorescence-enhancing matrix; d) irradiating the nucleotide to cause it to fluoresce; e) detecting the fluorescence; f) identifying the nucleote by its fluote; and g) repeating steps
Patent
Massively parallel sequencing of sorted polynucleotides
TL;DR: In this article, a method and materials for sorting polynucleotides with oligonucleotide tags is presented, which are capable of hybridizing to complementary oligomeric compounds consisting of subunits having enhanced binding strength and specificity as compared to natural oligon nucleotides.
Patent
Imaging immunoassay detection system with background compensation and its use
TL;DR: In this paper, an imaging immunoassay detection apparatus system and method capable of detecting multiple light emitting reactions from small volume samples simultaneously and quantifying the same was proposed, which can be used to detect the same types of events.
Patent
Flow imaging cytometer.
TL;DR: In this article, a flow imaging cytometer, in which an image capturing area for cells traveling in the flow of a specimen solution is constantly monitored to perform cell photography in an efficient manner, is improved so that fluorescent images of cells can be acquired with greater efficiency.
Related Papers (5)
Imaging and analyzing parameters of small moving objects such as cells
David Basiji,William E. Ortyn +1 more