Journal ArticleDOI
Chemical and ultrastructural studies on the cell walls of the yeastlike and mycelial forms of Histoplasma capsulatum.
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TLDR
In this article, the cell walls of the yeast-like (Y) and mycelial (M) forms of Histoplasma capsulatum G-184B were analyzed and it was shown that the Y form contained about 46.5% of β-glucan, 31.7% of galactomannan, 25.8% of chitin, and essentially no αglucans.Abstract:
Chemical and ultrastructural studies of the cell walls of the yeastlike (Y) and mycelial (M) forms ofHistoplasma capsulatum G-184B revealed that the Y form contained about 46.5% ofα-glucan, 31.0% ofβ-glucan, 7.7% of galactomannan and 11.5% of chitin, whereas the M form cell wall contained about 18.8% ofβ-glucan, 24.7% of galactomannan, 25.8% of chitin, and essentially noα-glucan. Theα-glucan of the Y form contained mainly anα-(1 → 3)-linkage. Theβ-glucans of both forms may have mainly aβ-(1 → 3)-linkage. Chitin microfibrils were located mainly in the inner portion of the cell walls of the Y and M forms, whereas theα-glucan fibers were observed only in the outer portion of the Y form cell wall.read more
Citations
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Journal ArticleDOI
Histoplasma capsulatum α-(1,3)-glucan blocks innate immune recognition by the β-glucan receptor
TL;DR: It is shown that α-(1,3)-glucan is present in the outermost layer of the Histoplasma capsulatum yeast cell wall and contributes to pathogenesis by concealing immunostimulatory β-glucans from detection by host phagocytic cells.
Journal ArticleDOI
RNA interference in Histoplasma capsulatum demonstrates a role for α‐(1,3)‐glucan in virulence
TL;DR: This work has developed a telomeric plasmid‐based system for silencing gene expression in Histoplasma by RNA interference (RNAi), and demonstrates for the first time that α‐(1,3)‐glucan is an important contributor to histoplasma virulence.
Journal ArticleDOI
Compounds active against cell walls of medically important fungi.
TL;DR: Compounds specifically interfering with chitin or beta-glucan synthesis have proven effective in studies of preclinical models of mycoses, though they appear to have a restricted spectrum of coverage.
Book ChapterDOI
Fungal Cell Walls: A Survey
TL;DR: The cell wall is commonly regarded as an assemblage of polymers, mainly polysaccharides, that occurs outside the plasma membrane of cells of plants, fungi, and bacteria that offers protection and, being the outermost cover of cells, often contains molecules involved in interaction between cells.
Journal ArticleDOI
Fungal Cell Wall β-1,3-Glucans Induce Clotting and Phenoloxidase Attachment to Foreign Surfaces of Crayfish Hemocyte Lysate
TL;DR: Two species of crayfish, Astacus astacus and Pacifastacus leniusculus could recognize components from fungal cell walls, i.e. β-1,3-glucans, since a clotting process and subsequent protein (phenoloxidase) attachment to many foreign surfaces were induced by these β-2,2- glucans.
References
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Journal ArticleDOI
Isolation, composition, and structure of cell walls of filamentous and yeast-like forms of Mucor rouxii.
TL;DR: Comparative studies were made on their composition and structure to explore possible morphogenetic implications and major quantitative differences were found in protein, purine-pyrimidine, and especially mannose content, all of which were higher in the yeast walls.
Journal ArticleDOI
Chitin and yeast budding. Localization of chitin in yeast bud scars.
Enrico Cabib,Blair Bowers +1 more
TL;DR: Yeast cells extracted with alkali and acetic acid revealed very thin cell envelopes with prominent bud scars in the shape of a shallow crater with a raised rim, which concluded that chitin is localized in a ring around the bud scar, sandwiched between two layers of glucan.
Journal ArticleDOI
Cell Wall Composition of the Yeast and Mycelial Forms of Paracoccidioides brasiliensis
TL;DR: Chemical analyses of the cell walls of the yeast (Y form) and mycelial forms (M form) of Paracoccidioides brasiliensis and Blastomyces dermatitidis revealed that their chemical composition is similar and depends on the form.
Journal ArticleDOI
Biochemical Studies on the Thermal Dimorphism of Paracoccidioides brasiliensis
TL;DR: The biochemical and morphological changes of the yeastlike (Y) form to the mycelial (M) form of Paracoccidioides brasiliensis were examined and a hypothesis for the production of the M form from the Y form is proposed.