Journal ArticleDOI
Co-culture of 1-cell mouse embryos on different cell supports
TLDR
Neither a genital tract origin, nor a hormonal contribution are strictly necessary for embryo co- culture, as already demonstrated by co-culture with trophoblastic tissue.Abstract:
The development of 1-cell mouse embryos in explanted oviducts, on mouse and bovine oviduct epithelial cells and on two established cell line supports is compared. The best rates of blastocyst formation were obtained using explanted oviducts; mouse and to a lesser extent, bovine oviduct epithelial cells allow good embryonic development, associated with high viability after transfer of the blastocysts obtained in co-culture. MDBK (from bovine kidney) and Vero (from Green monkey kidney) have been tested. MDBK allows high rates of blastocyst formation (67%) and the blastocysts obtained are viable. Vero does not allow the 2-cell block to be overcome. Maintenance of cell polarity for all the feeder layers did not improve embryo development. A preliminary study on the metabolic modifications induced by the feeder layers showed no modifications at all related to a decrease in glucose, an increase in lactate and early embryonic development. On the other hand, for the free amino acids, cellular supports with high embryotrophic activity seem to mimic tubal secretions, especially with a high level of glycine. Neither a genital tract origin, nor a hormonal contribution are strictly necessary for embryo co-culture, as already demonstrated by co-culture with trophoblastic tissue. Established cell lines, which are easy to handle and control, could be useful tools in embryo biotechnology.read more
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Journal ArticleDOI
Culture of in vitro produced bovine zygotes in vitro vs in vivo: Implications for early embryo development and quality
Brian P. Enright,Patrick Lonergan,Andras Dinnyes,Trudee Fair,F. Ward,Xiangzhong Yang,Maurice P. Boland +6 more
TL;DR: It is demonstrated that while bovine zygotes cultured in vitro are capable of rates of development similar to those of their in vivo cultured counterparts, there are significant differences in embryo cryosurvival.
Journal ArticleDOI
Mouse and bovine models for human IVF.
Yves Menezo,François Hérubel +1 more
TL;DR: Interactions between the embryo and the corpus luteum are similar in cows and humans, but mouse and human embryo implantations are closer.
Journal ArticleDOI
Role of colony stimulating factor-1 (CSF-1) and other lympho-hematopoietic growth factors in mouse pre-implantation development.
TL;DR: Mouse pre‐implantation development appears to be under the control of paracrine and autocrine growth factors, suggesting a partial overlap of the regulatory signals used by the reproductive and lympho‐hematopoietic systems.
Journal ArticleDOI
Cocultures: a new lead in embryo quality improvement for assisted reproduction.
TL;DR: The establishment, maintenance, and behavior of human tubal cell lines is first presented, followed by their use as cocultures for fertilization and cleavage of embryos and the currently available results of pregnancies after coculture are presented.
Book
The Fallopian Tube
TL;DR: The Fallopian Tube and Fertility Control Advances and Recent Trends are discussed.
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