Cryopreservation of transgenic mouse embryos by ultrarapid freezing
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The results indicated that the cryopreservation of embryos by ultrarapid freezing was valuable for sustaining transgenic mouse lines without genetic contaminations.Abstract:
Two transgenic mice lines were produced by introducing the rat GH antisense transgene and the chicken beta-actin promoter/firefly luciferase hybrid gene in our laboratory. We cryopreserved the transgenic embryos, obtained by fertilization in vitro with the sperm of hemizygous males for the transgene, by the ultrarapid freezing. The survival rates of cryopreserved 2-cell embryos were high (> 70%) at thawing in both lines and 53% and 16% of cryopreserved 2-cell embryos, respectively, developed to live young after transferring to oviducts of recipients. Each transgene was detected at about 40% of the live young from two transgenic lines. These results indicated that the cryopreservation of embryos by ultrarapid freezing was valuable for sustaining transgenic mouse lines without genetic contaminations.read more
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Journal ArticleDOI
Application of Laser-Assisted Zona Drilling to In Vitro Fertilization of Cryopreserved Mouse Oocytes with Spermatozoa from a Subfertile Transgenic Mouse
Masayuki Anzai,Megumi Nishiwaki,Miho Yanagi,Tatsuyuki Nakashima,Takehito Kaneko,Yoshitomo Taguchi,Mikiko Tokoro,Seung-Wook Shin,Tasuku Mitani,Hiromi Kato,Kazuya Matsumoto,Naomi Nakagata,Akira Iritani +12 more
TL;DR: Results indicate that laser-assisted zona drilling of oocytes combined with cryopreservation by vitrification may be a useful approach for large-scale production of in vitro fertilized embryos for managing transgenic mouse strains with reproductive disabilities such as subfertile sperm.
Journal ArticleDOI
Viability of transgenic rat embryos after freezing and thawing.
TL;DR: Two-step freezing of embryos derived from transgenic rats was an effective method for the long-term cryopreservation of transgene.
Journal ArticleDOI
Maintenance of Transgenes in Rats: The Contributions of Embryo Cryopreservation and Intracytoplasmic Sperm/Spermatid Injection
TL;DR: When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes.
Journal ArticleDOI
Vitrification of Transgenic Mouse Embryos: Effects of Cryopreservation on the Structure and Expression of Transgene
TL;DR: It was indicated that the vitrification procedure the authors employed does not affect the expression level of the transgene or its integration pattern in chromosomal DNA.